|本期目录/Table of Contents|

[1]史彩华,胡静荣,李传仁,等.内参基因在昆虫qRT-PCR中的研究进展[J].江苏农业科学,2017,45(07):1-7.
 Shi Caihua,et al.Research progress of reference gene for real-time quantitative reverse transcription PCR (qRT-PCR) of insects[J].Jiangsu Agricultural Sciences,2017,45(07):1-7.
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内参基因在昆虫qRT-PCR中的研究进展(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年07期
页码:
1-7
栏目:
专论与综述
出版日期:
2017-04-20

文章信息/Info

Title:
Research progress of reference gene for real-time quantitative reverse transcription PCR (qRT-PCR) of insects
作者:
史彩华 胡静荣 李传仁 王文凯
长江大学农学院,湖北荆州 434025
Author(s):
Shi Caihuaet al
关键词:
内参基因昆虫qRT-PCR进展
Keywords:
-
分类号:
S436.33
DOI:
-
文献标志码:
A
摘要:
实时荧光定量PCR(qRT-PCR)具有灵敏度高、特异性强等优点,是昆虫目标基因表达和转录分析最常用的技术手段之一。然而,为了消除样本在RNA产量、质量及逆转录效率上存在的差异,在进行qRT-PCR分析目标基因表达量时必须选择合适的“管家基因”作为内参基因。近年来,大量研究表明不同昆虫和不同试验条件选择的内参基因也不尽相同。因此,选择合适的内参基因是正确判断qRT-PCR结果分析的关键。从内参基因的选择、昆虫内参基因的研究和内参基因稳定性评价等几个方面进行综述,以期为研究者在将来的昆虫试验中选择合适的内参基因提供理论参考依据。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2016-02-16
基金项目:国家公益性行业(农业)科研专项(编号:201303027)。
作者简介:史彩华(1980—),男,湖北天门人,硕士,讲师,主要从事农业昆虫与害虫防治研究。E-mail:shicaihua1980@126.com。
通信作者:张友军,博士,研究员,主要从事蔬菜昆虫与害虫防治研究。E-mail:nxy2007@126.com。
更新日期/Last Update: 2017-04-05