|本期目录/Table of Contents|

[1]邱丹,杜芮萍,孟德凯,等.砷超富集植物蜈蚣草丛枝菌根观察方法的优化[J].江苏农业科学,2017,45(07):236-238.
 Qiu Dan,et al.Optimization of arbuscular mycorrhizal observation method of hyperaccumulator Pteris vittata L.[J].Jiangsu Agricultural Sciences,2017,45(07):236-238.
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砷超富集植物蜈蚣草丛枝菌根观察方法的优化(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年07期
页码:
236-238
栏目:
资源与环境
出版日期:
2017-04-20

文章信息/Info

Title:
Optimization of arbuscular mycorrhizal observation method of hyperaccumulator Pteris vittata L.
作者:
邱丹1 杜芮萍1 孟德凯1 黎宁2 顾明华1 王学礼1
1.广西大学农学院,广西南宁 530004;2.广西壮族自治区环境监测中心站,广西南宁 530028
Author(s):
Qiu Danet al
关键词:
超富集植物丛枝菌根蜈蚣草共生体系观察优化
Keywords:
-
分类号:
Q944.54;Q949.32
DOI:
-
文献标志码:
A
摘要:
为了便于蜈蚣草菌根结构的观察及侵染状况的测定,研究根系组织透明(脱色)流程优化及采用酸性品红乳酸液、曲利苯蓝、蓝黑醋酸墨水(北京牌)和纯黑醋酸墨水(Quink牌)4种染液处理对菌根染色效果的影响,以优化蜈蚣草丛枝菌根观察的脱色条件及染液染色方法。结果表明,采用20%KOH于90 ℃水浴60 min、碱性H2O2处理 45 min 的方法对根系的脱色效果最好;采用醋酸墨水染色,菌丝、泡囊、丛枝的染色效果明显。研究结果将为观察蜈蚣草菌根提供技术指导,方便测定真菌侵染状况,从而为进一步研究丛枝菌根真菌对砷在蜈蚣草体内的迁移转化提供方法指导。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-04-30
基金项目:广西自然科学基金(编号:2014GXNSFBA118223);广西特聘专家工程专项经费(编号:2013B015);广西科学研究与技术开发计划(编号:桂科合14125008-2-23)。
作者简介:邱丹(1991—),女,湖北武汉人,硕士研究生,主要从事植物修复技术研究。E-mail:2447496512@qq.com。
通信作者:王学礼,博士,讲师,主要从事重金属污染土壤修复研究。E-mail:wxl0524@126.com。
更新日期/Last Update: 2017-04-05