[1]张雪娇,王利平,赵振军,等.来源于西洋梨的苹果茎沟病毒分离物基因组分子特性研究[J].江苏农业科学,2017,45(18):43-47.
Zhang Xuejiao,et al.Molecular characteristics of genome of apple stem grooving virus isolated from European pear(Pyrus communis L.)[J].Jiangsu Agricultural Sciences,2017,45(18):43-47.
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来源于西洋梨的苹果茎沟病毒分离物基因组分子特性研究(PDF)
Zhang Xuejiao,et al.Molecular characteristics of genome of apple stem grooving virus isolated from European pear(Pyrus communis L.)[J].Jiangsu Agricultural Sciences,2017,45(18):43-47.
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第45卷
- 期数:
- 2017年18期
- 页码:
- 43-47
- 栏目:
- 生物技术
- 出版日期:
- 2017-09-20
文章信息/Info
- Title:
- Molecular characteristics of genome of apple stem grooving virus isolated from European pear(Pyrus communis L.)
- Author(s):
- Zhang Xuejiao; et al
- Keywords:
- -
- 分类号:
- S436.611.1+9
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 为了获得来源于西洋梨红贝雷沙寄主潜带苹果茎沟病毒(ASGV)分离物的基因组全长序列，并明确其分子特性，采用RT-PCR、RACE末端克隆及生物信息学方法，对ASGV分离物基因组全长进行序列测定，并对其序列特点进行分析。结果发现，来源于西洋梨的ASGV-HB分离物的基因组全长序列为6 496 nt(GenBank登录号：KU605672)，含有2个开放阅读框ORF1、ORF2及2个可变区Ⅵ、Ⅶ。序列分析表明ASGV-HB与GenBank登录的18个ASGV分离物的基因组全长核苷酸序列同源性为80.6%~87.7%；ORF1和ORF2编码的氨基酸同源性分别为843%~92.3%和94.4%~98.7%；Ⅵ和Ⅶ可变区的氨基酸序列同源性分别为22.9%~68.8%和48.8%~922%；系统发育树分析显示，来源于不同国家的相同寄主的ASGV分离物聚集为同一分支。结果表明，ASGV的分子变异无地域相关性，具有一定的寄主选择性，研究结果为进一步探究ASGV的群体遗传进化机制及其防治提供了重要的分子信息。
- Abstract:
- -
参考文献/References:
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备注/Memo
- 备注/Memo:
- 收稿日期：2016-05-07 基金项目：国家自然科学基金(编号：31201488)；国家梨产业技术体系(编号：nycytx-29-08)。 作者简介：张雪娇(1990—)，女，河南周口人，硕士，研究方向为植物分子遗传学。E-mail：yaqifeixue62@sina.com。 通信作者：崔百明，博士，副教授，研究方向为植物分子遗传学。E-mail：2247237543@qq.com。
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