|本期目录/Table of Contents|

[1]郭丽红,徐娅,郤秋霞,等.拟南芥热激因子AtHsfA1a在低温胁迫下对细胞程序性死亡中Caspase-3活性的影响[J].江苏农业科学,2017,45(21):24-27.
　Guo Lihong,et al.Effects of heat shock factor AtHsfA1a on Caspase-3 enzyme activity during programmed cell death under low temperature stress in Arabidopsis thaliana[J].Jiangsu Agricultural Sciences,2017,45(21):24-27.
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拟南芥热激因子AtHsfA1a在低温胁迫下对细胞程序性死亡中Caspase-3活性的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年第21期
页码:
24-27
栏目:
生物技术
出版日期:
2017-11-05

文章信息/Info

Title:
Effects of heat shock factor AtHsfA1a on Caspase-3 enzyme activity during programmed cell death under low temperature stress in Arabidopsis thaliana
作者:
郭丽红1; 2; 4;  徐娅2;  郤秋霞2;  李念2;  檀文涛2;  张学兰2
1.昆明学院/云南省高校特色生物资源开发与利用重点实验室，云南昆明 650214； 2.昆明学院/昆明市滇池水生态恢复重点实验室，
云南昆明 650214；3.昆明学院生命科学与技术系，云南昆明 650214； 4.昆明学院农学院，云南昆明 650214
Author(s):
Guo Lihong; et al
关键词:
拟南芥; 热激因子AtHsfA1a; 低温胁迫; 细胞程序性死亡; Caspase-3活性
Keywords:
-
分类号:
Q945.78
DOI:
-
文献标志码:
A
摘要:
为了研究拟南芥热激因子AtHsfA1a对低温胁迫下细胞程序性死亡(programmed cell death，简称PCD)中含半胱氨酸的天冬氨酸蛋白水解酶(cysteinyl aspartate specific protease，简称Caspase)活性的影响，进一步确定拟南芥热激因子AtHsfA1a与低温胁迫中PCD的关系。以热激因子AtHsfA1a不同基因型(野生型、基因沉默型)的拟南芥为材料，首先获得单细胞，于4 ℃处理1 h后，测定热激因子AtHsfA1a的表达量，发现基因沉默型中AtHsfA1a的表达量低于野生型。接着用4′，6-二脒基-2-苯基吲哚(4′，6-diamidino-2-phenylindole，简称DAPI)进行染色，在荧光显微镜下观察细胞形态，结果表明，低温胁迫后野生型未出现凋亡小体，基因沉默型的细胞出现了细胞凋亡小体。最后根据荧光底物Ac-DEVD-pNA的断裂程度测定Caspase-3活性，结果发现，低温处理后的拟南芥Caspase-3活性明显增强，而AtHsfA1a基因沉默型拟南芥Caspase-3活性比野生型拟南芥的高，说明低温胁迫下拟南芥AtHsfA1a能够抑制Caspase-3蛋白酶的活性。研究结果初步表明，在低温胁迫下拟南芥热激因子AtHsfA1a可以通过抑制 Caspase-3 蛋白酶的活性而对细胞程序性死亡有一定的抑制作用，这对于揭示植物耐逆境反应机制具有重要意义。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2016-06-02
基金项目：国家自然科学基金(编号：31260061、31060039)；云南省高校特色生物资源开发与利用重点实验室项目(编号：GXZD201601)。
作者简介：郭丽红(1971—)，女，云南大理人，博士，教授，主要从事植物生理学与分子生物学研究。E-mail：guolihong7122@163.com。
更新日期/Last Update: 2017-11-05