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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第46卷

期数:

2018年03期

页码:

80-85

栏目:

植物保护

出版日期:

2018-02-05

文章信息/Info

Title:

Lipopeptide proenzyme produced by Bacillus amyloliquefaciens is key material to control postharvest blue mold disease of apple

作者:

付瑞敏¹; 2;  常慧萍¹; 2;  邢文会¹; 2;  陈五岭²

1.河南教育学院生命科学系，河南郑州 450046； 2.西北大学生命科学学院，陕西西安 710069

Author(s):

Fu Ruimin; et al

关键词:

解淀粉芽胞杆菌; 青霉病; 丰原素; 突变株; 同源重组; 扩展青霉菌

Keywords:

-

分类号:

S182

DOI:

-

文献标志码:

A

摘要:

为构建解淀粉芽孢杆菌丰原素合成酶基因fenC缺失突变株，研究丰原素在苹果采后青霉病防治中所起的作用。根据同源重组的原理，采用PCR扩增fenC基因上、下游的同源序列，将其连接至穿梭质粒pMAD，构建含有壮观霉素抗性基因(spc)的同源重组质粒pMAD-Δfen，通过电转化技术，将其转至解淀粉芽孢杆菌BA-16-8，通过抗生素抗性筛选、蓝白斑筛选和高温培养筛选出解淀粉芽孢杆菌fenC基因敲除突变株BA-16-8-Δfen。通过高效液相色谱(high performance liquid chromatography，简称HPLC)分析、质谱鉴定和抑菌性试验检测敲除fenC基因对解淀粉芽孢杆菌防治青霉病的影响。结果表明，与野生株相比，突变株菌株细胞及其所产抗生素脂肽对扩展青霉的防治效果显著降低。说明丰原素在解淀粉芽孢杆菌BA-16-8防治青霉病中发挥主要作用。

Abstract:

-

参考文献/References:

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相似文献/References:

[1]窦勇,胡佩红,田林双,等.多聚赖氨酸对梨果采后青霉病防治及其抗性诱导机制[J].江苏农业科学,2022,50(14):177.
　Dou Yong,et al.Control and resistance induction mechanism of polylysine to postharvest penicillium in pear fruit[J].Jiangsu Agricultural Sciences,2022,50(03):177.

备注/Memo

备注/Memo:

收稿日期：2016-08-26
基金项目：国家农业科技成果转化资金(编号：2012GB2G000451)；河南省科技计划(编号：132102310253)；河南省高等学校重点科研项目(编号：15B180002)；陕西省重大科技创新项目(编号：2009ZKC04-16)；河南省基础与前沿技术研究项目(编号：152300410092)；河南教育学院青年科研课题(编号：20100103)。
作者简介：付瑞敏(1981—)，女，河南郑州人，博士研究生，讲师，主要从事农业及食品微生物研究。E-m

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更新日期/Last Update: 2018-02-05