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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第46卷

期数:

2018年07期

页码:

162-166

栏目:

畜牧兽医与水产蚕桑

出版日期:

2018-04-05

文章信息/Info

Title:

Separation of beta hemolytic Aeromonas hydrophila strain AH from Scleropages formosus and its effect on iron metabolism

作者:

余文杰¹;  陈观水²

1.漳州职业技术学院食品与生物工程系，福建漳州 363000； 2.福建农林大学生命科学学院，福建福州 350001

Author(s):

Yu Wenjie; et al

关键词:

嗜水气单胞菌; 红龙鱼; hepc基因; jak3基因; il-6基因; 铁代谢

Keywords:

-

分类号:

S942.1

DOI:

-

文献标志码:

A

摘要:

对具有典型败血症病症的染病红龙鱼进行病原菌分离鉴定分析。从其肝脏中分离得到病原菌菌株AH，经VITEK-32全自动细菌分析仪鉴定为嗜水气单胞菌。通过多重PCR检测确认该嗜水气单胞菌AH属于β-溶血性嗜水气单胞菌，含有气溶素基因(aerA)和溶血素基因(ahh1)。此外研究嗜水气单胞菌AH侵染红龙鱼后对其机体铁代谢的影响，通过腹腔注射嗜水气单胞菌AH，在侵染后不同时间点收集红龙鱼的血液和肝脏组织，分别采用分光光度计法和电感耦合等离子体原子发射光谱（inductively coupled plasma-atomic emission specrometry，简称ICP-AES）法检测红龙鱼血清和肝脏中的铁含量，同时采用实时荧光定量PCR法对铁调素基因(hepc)、白细胞介素基因(il-6)、JAK/STAT 信号通路的蛋白质酪氨酸激酶3基因(jak3)、信号转导子和转录激活因子3基因(stat3)表达量进行检测。结果表明，侵染嗜水气单胞菌AH后，红龙鱼血清中铁浓度明显降低，在24、48h时显著低于对照组，总铁结合力有所上升，但没有达到显著水平；肝脏中铁含量相对于对照组差异没有达到显著水平，但仍有明显升高。肝脏中hepc基因的表达量在一定时间范围内显著上升；il-6、jak3和stat3基因表达量开始均有所上调，随后有所下降，但在各时间点的表达量均高于对照。由结果可知，机体通过调节铁调素基因(hepc)等铁相关基因的表达，进而降低自身游离铁含量，并增加储存铁含量来应对细菌的侵染。

Abstract:

-

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备注/Memo

备注/Memo:

红龙鱼源β-溶血性嗜水气单胞菌AH的分离
及其对机体铁代谢的影响

常用功能

更新日期/Last Update: 2018-04-05