|本期目录/Table of Contents|

[1]王进军,薛藩.虾过敏DNA疫苗的设计和构建[J].江苏农业科学,2018,46(15):34-37.
　Wang Jinjun,et al.Design and construction of DNA vaccine against allergy caused by shrimp[J].Jiangsu Agricultural Sciences,2018,46(15):34-37.
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虾过敏DNA疫苗的设计和构建(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第15期
页码:
34-37
栏目:
生物技术
出版日期:
2018-08-05

文章信息/Info

Title:
Design and construction of DNA vaccine against allergy caused by shrimp
作者:
王进军;  薛藩
扬州大学环境科学与工程学院，江苏扬州 225127
Author(s):
Wang Jinjun; et al
关键词:
虾过敏; 抗原表位; 克隆; 真核表达质粒; DNA疫苗
Keywords:
-
分类号:
R392.7
DOI:
-
文献标志码:
A
摘要:
通过“一珠一化合物”方法筛选虾过敏的抗原表位，加上免疫元件构建重组真核表达质粒。取对虾过敏患者的血清，纯化抗体。用所得抗体从耦联氨基酸多肽库中筛选出不同长度的多肽20个。在每个多肽前后加入免疫元件(KOZAK序列、Th-PADRE、CpG1、Th-P18mn、CpG2、tPA前导序列、多肽加尾信号)，根据密码子表和JCAT网站选出适宜在小鼠内表达的DNA序列。按重叠PCR的方法设计6个20 bp的核苷酸链，通过PCR获得全长片段，双酶切连接入真核表达载体，构建重组表达质粒。经测序分析，证明成功构建虾抗原表位的重组表达质粒pCIneo-TM。本研究为虾过敏抗原表位在真核系统的表达以及已建立的虾过敏小鼠动物模型奠定了良好基础，可为虾过敏的治疗提供新的方法。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2017-03-06
基金项目：教育部留学回国人员科研启动基金(编号：20151098)；江苏省扬州市基础研究计划(编号：YZ2015098)；扬州大学高层次人才科研启动基金。
作者简介：王进军(1979—)，男，江苏海安人，博士，副教授，主要从事微生物学的相关研究。Email：wangjinjun@yzu.edu.cn。
更新日期/Last Update: 2018-08-05