|本期目录/Table of Contents|

[1]谷青,赵冬梅,张岱,等.北方一作区马铃薯早疫病病菌群体SSR遗传结构分析[J].江苏农业科学,2018,46(19):88-92.
 Gu Qing,et al.Population structure analysis of potato early blight based on SSR in northern China[J].Jiangsu Agricultural Sciences,2018,46(19):88-92.
点击复制

北方一作区马铃薯早疫病病菌群体SSR遗传结构分析(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第19期
页码:
88-92
栏目:
植物保护
出版日期:
2018-10-05

文章信息/Info

Title:
Population structure analysis of potato early blight based on SSR in northern China
作者:
谷青 赵冬梅 张岱 何佳昱 杨志辉 朱杰华
河北农业大学植物保护学院,河北保定 071000
Author(s):
Gu Qinget al
关键词:
马铃薯早疫病茄链格孢遗传多样性SSR分子标记
Keywords:
-
分类号:
S435.32
DOI:
-
文献标志码:
A
摘要:
为明确北方一作区马铃薯早疫病病菌不同地理群体的遗传结构,利用5对简单重复序列(simple sequence repeat,简称SSR)引物对河北、黑龙江、辽宁、内蒙古和宁夏5个省(自治区)的74株马铃薯早疫病病菌进行遗传多样性分析。共检测出26个等位基因,45个基因型,每对引物的等位基因数为3~7个,平均为5.2个。分析结果显示,5省(自治区)的早疫病病菌菌株群体之间相似度都很高,处于0.900 6~0.988 9之间。相较而言,河北群体和辽宁群体的亲缘关系较近,内蒙古群体和黑龙江群体的亲缘关系较近,宁夏群体单独为1个分支,表明SSR基因型与菌株的地理来源有相关性。研究还发现,在被测的45个基因型中,仅出现1次的基因型有37个,占82.2%,说明群体遗传多样性高,推测是准性生殖和人类介导的基因流动共同作用的结果。
Abstract:
-

参考文献/References:

[1]Wang B,Ma Y,Zhang Z,et al. Potato viruses in China[J]. Crop Protection,2011,30(9):1117-1123.
[2]Pasche J S,Piche L M,Gudmestad N C. Effect of the F129L mutation in Alternaria solani on fungicides affecting mitochondrial respiration[J]. Plant Disease,2005,81230(3):269-278.
[3]Olanya O M,Honeycutt C W,Larkin R P,et al. The effect of cropping systems and irrigation management on development of potato early blight[J]. Journal of General Plant Pathology,2009,75(4):267-275.
[4]Parker I M,Gilbert G S. The evolutionary ecology of novel plant-pathogen interactions[J]. Annual Review of Ecology Evolution & Systematics,2004,35:675-700.
[5]Zhan J,Thrall P H,Burdon J J. Achieving sustainable plant disease management through evolutionary principles[J]. Trends in Plant Science,2014,19(9):570-575.
[6]Sharma T R,Tewari J P. RAPD analysis of three Alternaria species pathogenic to crucifers[J]. Mycological Research,1998,102(7):807-814.
[7]张福光. 河北省早疫病菌群体结构研究及马铃薯栽培品种对早疫病的抗性评价[D]. 保定:河北农业大学,2011.
[8]Tautz D. Hypervariability of simple sequences as a general source for polymorphic DNA markers[J]. Nucleic Acids Research,1989,17(16):6463-6471.
[9]Varshney R K,Graner A,Sorrells M E. Genic microsatellite markers in plants:features and applications[J]. Trends in Biotechnology,2005,23(1):48-55.
[10]Méndez-Vigo B,Gomaa N H,Alonso-Blanco C,et al. Among- and within-population variation in flowering time of Iberian Arabidopsis thaliana estimated in field and glasshouse conditions[J]. New Phytologist,2013,197(4):1332-1343.
[11]Meng J W,Zhu W,He M H,et al. High genotype diversity and lack of isolation by distance in the Alternaria solani populations from China[J]. Plant Pathology,2015,64(2):434-441.
[12]张福光,杨志辉,朱杰华,等. 河北省马铃薯早疫病菌群体遗传结构的研究[J]. 菌物学报,2012,31(1):40-49.
[13]Francis C Y,Rong C Y,Boyle T. POPGENE,Microsoft windows based freeware for population genetic analysis[Z]//Molecular biology and biotechnology centre. Edmonton,Canada:University of Alberta,1999.
[14]Tamura K,Stecher G,Peterson D,et al. MEGA6:molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology Evolution,2013,30(12):2725-2729.
[15]Rohlf F J. NTSYS-PC,Numerical taxonomy system for the PC ExeterSoftware,Version 2.1d. Exeter Software[Z]. Setauket,New York. 2000.
[16]Brown J K M. The choice of molecular marker methods for population genetic studies of plant pathogens[J]. New Phytologist,1996,133(1):183-195.
[17]Cooke D E L,Lees A K. Markers,old and new,for examining Phytophthora infestans diversity[J]. Plant Pathology,2004,53(6):692-704.
[18]Kakvan N,Zamanizadeh H,Morid B,et al. Study on pathogenic and genetic diversity of Alternaria alternata isolated from citrus hybrids of Iran,based on RAPD-PCR technique[J]. European Journal of Experimental Biology,2012,2(3):570-576.
[19]Ghasemloee M,Niazmand A R. Genetic diversity of Alternaria alternata associated with Citrus spp. in southern Iran based on RAPD-PCR[J]. Journal of Pure and Applied Microbiology,2015,9(1):473-482.
[20]Stewart J E,Kawabe M,Abdo Z,et al. Contrasting codon usage patterns and purifying selection at the mating locus in putatively asexual Alternaria fungal species[J]. PLoS One,2011,6(5):e20083.

相似文献/References:

[1]刘华娇,谷青,赵冬梅,等.单孢继代培养对茄链格孢生长和产孢的影响[J].江苏农业科学,2019,47(09):160.
 Liu Huajiao,et al.Effects of subculturing on growth and sporulation of Alternaria solani[J].Jiangsu Agricultural Sciences,2019,47(19):160.

备注/Memo

备注/Memo:
收稿日期:2017-02-24
基金项目:现代农业产业技术体系建设专项(编号:CARS-10-P12)。
作者简介:谷青(1991—),女,河北石家庄人,硕士研究生,主要从事植物病理学研究。E-mail:guqing0708@163.com。
通信作者:朱杰华,硕士,教授,博士生导师,主要从事植物病理学研究。E-mail:zhujiehua356@126.com。
更新日期/Last Update: 2018-10-05