[1]孔维欢,熊燊源,代蓉,等.TLR4基因在绵羊不同生理状态下子宫内膜组织中的mRNA表达[J].江苏农业科学,2019,47(01):35-39.
Kong Weihuan,et al.mRNA expression of TLR4 gene in ovine endometrium under different physiological conditions[J].Jiangsu Agricultural Sciences,2019,47(01):35-39.
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TLR4基因在绵羊不同生理状态下
子宫内膜组织中的mRNA表达(PDF)
Kong Weihuan,et al.mRNA expression of TLR4 gene in ovine endometrium under different physiological conditions[J].Jiangsu Agricultural Sciences,2019,47(01):35-39.
子宫内膜组织中的mRNA表达(PDF)
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第47卷
- 期数:
- 2019年第01期
- 页码:
- 35-39
- 栏目:
- 生物技术
- 出版日期:
- 2019-01-05
文章信息/Info
- Title:
- mRNA expression of TLR4 gene in ovine endometrium under different physiological conditions
- Author(s):
- Kong Weihuan; et al
- Keywords:
- -
- 分类号:
- S826.1
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 为了研究Toll样受体4(Toll-like receptor 4，TLR4)在绵羊胚胎附植期和发情周期子宫内膜中mRNA的表达模式。采用半定量RT-PCR和实时荧光定量RT-PCR分别检测妊娠21 d母羊全身组织和妊娠9、13、17、21、25 d 的母羊子宫内膜组织TLR4基因的表达以及绵羊卵泡期与黄体期母羊子宫内膜组织TLR4基因的表达。结果表明，TLR4基因在肝脏、脾脏、卵巢、输卵管、子宫、小肠、膀胱、肌肉及松果体等组织出现表达，其中在输卵管、卵巢、子宫、膀胱、脾脏及松果体中表达量较高，在肝脏、小肠、肌肉中表达较弱，而在心脏、垂体、脂肪等组织中不表达。从妊娠9 d开始，绵羊胚胎TLR4基因表达量呈逐渐下降趋势；至胚胎附植点17 d，TLR4基因表达量最低，之后又呈现上升趋势，到25 d达到峰值。不管是在细毛羊还是在湖羊子宫内膜组织中检测，TLR4基因黄体期的表达水平均显著高于卵泡期(P<0.05)。说明TLR4的mRNA表达具有组织特异性，在胚胎附植期绵羊子宫内膜中呈现先低后高的趋势，以及其在绵羊发情周期中表达出现黄体期显著高于卵泡期现象，也初步说明了TLR4可能在胚胎附植早期和发情周期中均发挥一定作用。
- Abstract:
- -
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备注/Memo
- 备注/Memo:
-
收稿日期：2017-09-15
基金项目：国家重点研发计划(编号：2017YFD0501904)；新疆生产建设兵团科技攻关与成果转化计划(编号：2016AC027)；国家绒毛用羊产业技术体系建设项目(编号：CARS-40-07)。
作者简介：孔维欢(1989—)，男，河南濮阳人，硕士，主要从事动物遗传育种与繁殖研究。E-mail：919282632@qq.com
通信作者：石国庆，博士，研究员，博士生导师，主要从事绵羊繁育与家畜胚胎生物技术研究，E-mail：nkkxyxms
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