|本期目录/Table of Contents|

[1]关素华,曾庆辉,张晓飞,等.德式乳酸杆菌对黄河鲤抗胁迫能力的影响[J].江苏农业科学,2019,47(01):166-170.
 Guan Suhua,et al.Effect of Lactobacillus delbrueckii on anti-stress capacity of Cyprinus carpio var. Huanghe[J].Jiangsu Agricultural Sciences,2019,47(01):166-170.
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德式乳酸杆菌对黄河鲤抗胁迫能力的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第01期
页码:
166-170
栏目:
畜牧兽医与水产蚕桑
出版日期:
2019-01-05

文章信息/Info

Title:
Effect of Lactobacillus delbrueckii on anti-stress capacity of Cyprinus carpio var. Huanghe
作者:
关素华 曾庆辉 张晓飞 李静 李帅 张安东 马玉兴 张春暖
河南科技大学动物科技学院,河南洛阳 471003
Author(s):
Guan Suhuaet al
关键词:
德式乳酸杆菌黄河鲤免疫应激抗氧化
Keywords:
-
分类号:
S942.1
DOI:
-
文献标志码:
A
摘要:
为研究德式乳酸杆菌对黄河鲤应激前后抗应激指标、血液免疫指标、抗氧化指标和HSP70、HSP90基因表达的影响,选取黄河鲤450尾,随机分为5组(每组3个平行,每个平行30尾鱼),分别投喂在基础饲料中添加不同浓度乳酸菌(0、1×105、1×106、1×107、1×108 CFU/g)的日粮,养殖8周后,进行拥挤胁迫。试验结果表明:应激前,皮质醇浓度在1×106、1×107 CFU/g乳酸菌组显著低于对照组(P<0.05),血糖和乳酸浓度在试验组显著低于对照组(P<005),溶菌酶活性在1×106、1×107 CFU/g乳酸菌组显著高于对照组(P<0.05),C3和C4在1×106 CFU/g乳酸菌组显著高于对照组(P<0.05)。应激后,血液皮质醇、血糖和乳酸浓度都有不同程度的升高,这些指标在添加乳酸菌组的含量均显著低于对照组(P<0.05),血液溶菌酶活性、C3和C4浓度也有升高趋势,且在1×106 CFU/g乳酸菌组显著高于对照组(P<0.05);应激前后SOD和CAT活性在1×106 CFU/g乳酸菌组显著高于对照组(P<0.05),MDA含量呈相反的趋势,在1×106、1×107 CFU/g乳酸菌组显著低于对照组(P<0.05),应激使HSP70和HSP90基因表达均有升高趋势,在应激前后试验组的HSP70和HSP90基因表达量大部分都显著高于对照组(P<0.05)。该研究结果显示,饲料中添加1×106~1×107 CFU/g 德式乳酸菌能提高黄河鲤的免疫力、抗氧化和抗应激的能力。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2017-09-22
基金项目:河南科技大学大学生研究训练计划(SRTP)(编号:2017313);河南科技大学博士科研启动资金(编号:13480074)。
作者简介:关素华(1994—),女,河南周口人,从事水产动物营养与饲料研究。E-mail:18438615396@163.com。
通信作者:张春暖,博士,讲师,主要从事水产动物营养与饲料研究。E-mail:zhangchunnuan12@163.com。
更新日期/Last Update: 2019-01-05