|本期目录/Table of Contents|

[1]曹燕篆,王小娟,袁旭峰,等.利用废纸产纤维素酶复合菌系的筛选及性质研究[J].江苏农业科学,2019,47(01):237-242.
　Cao Yanzhuan,et al.Study on screening and characterization of cellulase-compacting bacterial strains from waste paper[J].Jiangsu Agricultural Sciences,2019,47(01):237-242.
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利用废纸产纤维素酶复合菌系的筛选及性质研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第01期
页码:
237-242
栏目:
资源与环境
出版日期:
2019-01-05

文章信息/Info

Title:
Study on screening and characterization of cellulase-compacting bacterial strains from waste paper
作者:
曹燕篆1; 2;  王小娟2;  袁旭峰2;  王小芬2;  崔宗均2
1.山西农业大学资源与环境学院，山西太谷 030801； 2.中国农业大学农学院，北京 100193
Author(s):
Cao Yanzhuan; et al
关键词:
复合菌系; 废纸分解; 纤维素酶; 微生物组成
Keywords:
-
分类号:
S182；X705
DOI:
-
文献标志码:
A
摘要:
为了获得1组能够高效分解废纸并产胞外纤维素酶的复合菌系，采用外淘汰法，在常温条件下以土壤及枯枝落叶为菌源，以废打印纸为唯一碳源，筛选得到1组复合菌系PSD。结果表明，该复合菌系能够在6 d内分解纸总质量的71%，其中纤维素降解率为72%，半纤维素降解率为48%。此外，羧甲基纤维素酶活性在分解3 d时达到最高值 3.26 U/mL，半纤维素酶活性在分解4 d时达到最高值6.37 U/mL。当培养温度在25~35 ℃，培养基pH值在 6.0~80，培养基为Hutchinson时，复合菌系具有较好的纸分解效果和产酶活性。利用16S及26S rRNA克隆技术分析微生物组成结果表明，该复合菌系由细菌和真菌组成，其中细菌主要包括厚壁菌门、变形菌门及拟杆菌门，真菌主要包括波氏假性霉样菌(Pseudallescheria boydii)。由研究结果可知，得到的复合菌系PSD能够有效分解废纸并同时分泌纤维素酶，对打印纸废弃物的分解及资源利用具有一定的应用意义。
Abstract:
-

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相似文献/References:

[1]何水清,艾士奇,王建豪,等.木质纤维素分解复合菌系的分解特性与细菌组成多样性分析[J].江苏农业科学,2017,45(16):241.
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备注/Memo

备注/Memo:
收稿日期：2017-09-11
基金项目：国家科技支撑计划(编号：2012BAD14B06)；山西省青年科技研究基金(编号：201601D021105)；山西省回国留学人员科研资金(编号：2016-072)；山西农业大学引进人才科研启动项目(编号：2014YJ17)。
作者简介：曹燕篆(1984—)，女，山西太原人，博士，讲师，主要从事废弃物资源利用及微生物生态学研究。Tel：(0354)6288322；E-mail：caoyanzhuan@126.com。
通信作者：崔宗均
更新日期/Last Update: 2019-01-05