|本期目录/Table of Contents|

[1]祝友朋,陈摇,韩长志.胶孢炭疽菌黑色素合成途径中漆酶蛋白生物信息学分析[J].江苏农业科学,2019,47(06):48-51.
 Zhu Youpeng,et al.Bioinformatics analysis of laccase protein in melanin synthesis pathway of Colletotrichum gloeosporioides[J].Jiangsu Agricultural Sciences,2019,47(06):48-51.
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胶孢炭疽菌黑色素合成途径中漆酶
蛋白生物信息学分析
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第06期
页码:
48-51
栏目:
生物技术
出版日期:
2019-04-05

文章信息/Info

Title:
Bioinformatics analysis of laccase protein in melanin synthesis pathway of Colletotrichum gloeosporioides
作者:
祝友朋 陈摇 韩长志
西南林业大学生物多样性保护与利用学院云南省森林灾害预警与控制重点实验室,云南昆明 650224
Author(s):
Zhu Youpenget al
关键词:
农林植物胶孢炭疽菌黑色素漆酶生物信息学遗传进化
Keywords:
-
分类号:
S432.4+4
DOI:
-
文献标志码:
A
摘要:
胶孢炭疽菌可以侵染桃、核桃等诸多重要农林植物,它可引起生产上诸多经济树种的炭疽病,严重威胁着各国农林业的健康生产。植物病原丝状真菌中黑色素合成途径中关键环节漆酶已经在稻瘟病菌、玉米大斑病菌等进行了较为深入的研究,然而尚未见有关胶孢炭疽菌黑色素合成途径中关键环节漆酶的生物信息学分析报道。本研究以胶孢炭疽菌中漆酶Cg14LAC氨基酸序列为基础,通过TargetP、SignalP、ProtComp、Phyre、big-PI Fungal Predictor、SMART、TMHMM等生物信息学分析工具对上述序列开展蛋白质亚细胞定位、细胞信号肽、二级结构、锚定位点、保守结构域、跨膜结构域等进行分析,同时对上述序列开展与炭疽菌属不同真菌遗传进化关系分析。研究结果为进一步深入开展该蛋白在致病过程中所具有的功能研究打下坚实的理论基础。
Abstract:
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参考文献/References:

[1]韩长志. 胶孢炭疽病菌的研究进展[J]. 华北农学报,2012,27(增刊1):386-389.
[2]韩长志. 胶孢炭疽菌侵染过程相关基因研究进展[J]. 广东农业科学,2014,41(9):165-169.
[3]OConnell R J,Thon M R,Hacquard S,et al. Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses[J]. Nature Genetics,2012,44(9):1060-1065.
[4]韩长志. 胶孢炭疽菌RGS蛋白生物信息学分析[J]. 河南师范大学学报(自然科学版),2015(1):116-122.
[5]贾慧,孟庆江,李志勇,等. 玉米大斑病菌黑色素合成酶基因的全基因组定位及表达模式分析[J]. 中国农业科学,2015,48(14):2767-2776.
[6]Widsten P,Kandelbauer A. Laccase applications in the forest products industry:a review[J]. Enzyme and Microbial Technology,2008,42(4):293-307.
[7]陈新. 稻瘟病菌两个漆酶基因功能分析[D]. 福州:福建农林大学,2008.
[8]Sbaghi M,Jeandet P,Bessis R,et al. Degradation of stilbene‐type phytoalexins in relation to the pathogenicity of Botrytis cinerea to grapevines[J]. Plant Pathology,2010,45(1):139-144.
[9]孟川. 玉米大斑病菌StLAC2基因与黑色素合成及致病性关系研究[D]. 保定:河北农业大学,2013.
[10]Caero D C,Roncero M I. Functional analyses of laccase genes from Fusarium oxysporum[J]. Phytopathology,2008,98(5):509-518.
[11]Lin S Y,Okuda S,Ikeda K,et al. LAC2 encoding a secreted laccase is involved in appressorial melanization and conidial pigmentation in Colletotrichum orbiculare[J]. Molecular Plant-Microbe Interactions,2012,25(12):1552-1561.
[12]Tsuji G,Fujikawa J,Ishida H,et al. Laccase gene LAC1 of colletotrichum lagenarium is not essential for melanin biosynthesis and pathogenicity[J]. Journal of General Plant Pathology,2001,67(3):182-190.
[13]韦运谢,张贺,刘晓妹,等. 芒果胶孢炭疽菌漆酶lac1与致病力的相关性分析[J]. 中国植保导刊,2016,36(11):5-10.
[14]韦运谢,蒲金基,张贺,等. 胶孢炭疽菌漆酶基因Lac2的序列特征与表达分析[J]. 江苏农业科学,2015,43(9):35-38.
[15]李娅,韩长志. 云南省核桃产业发展现状及对策分析[J]. 经济林研究,2012,30(4):162-167.
[16]曹志艳. 玉米大斑病菌黑色素合成途径相关基因的克隆及功能分析[D]. 保定:河北农业大学,2009.
[17]Emanuelsson O,Brunak S,von Heijne G,et al. Locating proteins in the cell using TargetP,SignalP and related tools[J]. Nature Protocols,2007,2(4):953-971.
[18]Dyrlv-Bendtsen J,Nielsen H,von Heijne G,et al. Improved prediction of signal peptides:SignalP 3.0[J]. Journal of Molecular Biology,2004,340(4):783-795.
[19]Emanuelsson O,Nielsen H,Brunak S,et al. Predicting subcellular localization of proteins based on their N-terminal amino acid sequence[J]. Journal of Molecular Biology,2000,300(4):1005-1016.
[20]Eisenhaber B,Schneider G,Wildpaner M,et al. A sensitive predictor for potential GPI lipid modification sites in fungal protein sequences and its application to genome-wide studies for Aspergillus nidulans,Candida albicans,Neurospora crassa,Saccharomyces cerevisiae and Schizosaccharomyces pombe[J]. Journal of Molecular Biology,2004,337(2):243-253.
[21]Gasteiger E,Hoogland C,Gattiker A,et al. Protein identification and analysis tools on the ExPASy server[J]. The proteomics protocols handbook:Springer,2005:571-607.
[22]Schultz J,Milpetz F,Bork P,et al. SMART,a simple modular architecture research tool:identification of signaling domains[J]. Proceedings of the National Academy of Sciences of the United States of America,1998,95(11):5857-5864.
[23]Letunic I,Doerks T,Bork P. SMART 7:recent updates to the protein domain annotation resource[J]. Nucleic Acids Research,2012,40:D302-D305.
[24]Tusnady G E,Simon I. The HMMTOP transmembrane topology prediction server[J]. Bioinformatics,2001,17(9):849-850.
[25]Kelley L A,Mezulis S,Yates C M,et al. The phyre2 web portal for protein modeling,prediction and analysis[J]. Nature Protocols,2015,10(6):845-858.
[26]Thompson J D,Gibson T J,Higgins D G . Multiple sequence alignment using ClustalW and ClustalX.[J]. Curr Protoc Bioinformatics,2002,Chapter 2(Unit 2):Unit 2.3.
[27]Tamura K,Peterson D,Peterson N,et al. MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J]. Molecular Biology and Evolution,2011,28(10):2731-2739.
[28]Kelley L A,Sternberg M J. Protein structure prediction on the Web:a case study using the Phyre server[J]. Nature Protocols,2009,4(3):363-371.
[29]Buchan D W A,Minneci F,Nugent T C O,et al. Scalable web services for the PSIPRED Protein Analysis Workbench[J]. Nucleic Acids Research,2013,41(W1):W349-W357.
[30]刘邮洲,陈夕军,尹小乐,等. 23株芽胞杆菌及其脂肽类化合物抑菌活性比较[J]. 江苏农业学报,2017,33(3):533-542.
[31]柳志强,李乔曼,徐爽,等. 催吐萝芙木内生菌Bacillus subtilis LYM3的抑菌活性产物[J]. 江苏农业学报,2017,33(1):67-72.

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 Wei Yunxie,et al.Sequence characteristics and expression analysis of laccase gene Lac2 from Colletotrichum gloeosporioides[J].Jiangsu Agricultural Sciences,2015,43(06):35.
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备注/Memo

备注/Memo:
收稿日期:2017-12-13
基金项目:国家自然科学基金(编号:31560211);国家级大学生创新创业训练计划(编号:201610677001);云南省高等学校大学生创新创业训练计划;云南省森林灾害预警与控制重点实验室开放基金(编号:ZK150004);云南省高校优势特色重点学科(生物学)建设项目(编号:50097505)。
作者简介:祝友朋(1996—),男,云南曲靖人,E-mail:3420204485@qq.com;共同第一作者:陈摇(1995—),女,云南昆明人,E-mail:2058278171@qq.com。
通信作者:韩长志,博士,副教授,研究方向为经济林木病害生物防治与真菌分子生物学。E-mail:hanchangzhi2010@163.com。
更新日期/Last Update: 2019-03-20