[1]薄明森,吕杰,李兴海,等.新疆准东油田石油污染土壤萘双加氧酶基因的多态性[J].江苏农业科学,2019,47(09):110-114.
Bo Mingsen,et al.Polymorphism of naphthalene dioxygenase gene in oil-contaminated soils of Zhundong oilfield in Xinjiang area[J].Jiangsu Agricultural Sciences,2019,47(09):110-114.
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新疆准东油田石油污染土壤萘双加氧酶基因的多态性(PDF)
Bo Mingsen,et al.Polymorphism of naphthalene dioxygenase gene in oil-contaminated soils of Zhundong oilfield in Xinjiang area[J].Jiangsu Agricultural Sciences,2019,47(09):110-114.
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第47卷
- 期数:
- 2019年第09期
- 页码:
- 110-114
- 栏目:
- 生物技术
- 出版日期:
- 2019-05-27
文章信息/Info
- Title:
- Polymorphism of naphthalene dioxygenase gene in oil-contaminated soils of Zhundong oilfield in Xinjiang area
- Author(s):
- Bo Mingsen; et al
- Keywords:
- -
- 分类号:
- S151.9+5；S182；X53
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 为了分析新疆准东油田石油污染土壤中萘双加氧酶功能基因的多态性，采用无机盐培养基，以萘为唯一的碳源物质，对准东油田石油污染土壤中的萘降解菌进行液体富集培养，用高效液相色谱法(high performance liquid chromatography，简称HPLC)对降解产物进行检测，同时采用萘双加氧酶基因通用引物对萘双加氧酶基因片段进行扩增、测序及分析。高效液相色谱检测结果显示，萘被降解成小分子产物，证明液体培养基均富集培养获得了萘降解菌群。从准东油田共分离得到了49条萘双加氧酶基因片段，共编码35条氨基酸序列，GenBank登录号为KY304781~KY304829。获得的萘双加氧酶基因系统发育树结果显示，研究获得的萘双加氧酶基因分为4个类群。通过高效液相色谱分析及萘双加氧酶基因的分子检测，证明准东油田石油污染土壤中含有萘降解菌株，这些萘降解菌株是可以通过液体富集培养获得的，并且可以通过固体平板获得降解萘的单菌株。
- Abstract:
- -
参考文献/References:
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备注/Memo
- 备注/Memo:
-
收稿日期：2018-01-23
基金项目：国家自然科学基金(编号：31360131)；新疆大学大学生创新训练计划(编号：201510755170)。
作者简介：薄明森(1992—)，男，河南方城人，硕士研究生，主要从事分子生态学相关研究工作。E-mail：mingsenbo@163.com。
通信作者：马媛，博士，副教授，主要从事分子生态学、环境微生物相关研究工作。E-mail：xjmayuan@sina.com。
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