|本期目录/Table of Contents|

[1]杨郑州,黄柳芳,谢晓娜,等.叶疫病菌侵染芒果后叶片细胞壁降解酶活性测定[J].江苏农业科学,2019,47(10):138-141.
 Yang Zhengzhou,et al.Study on determination of cell wall degrading enzyme activity in mango leaves infected by leaf blight[J].Jiangsu Agricultural Sciences,2019,47(10):138-141.
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叶疫病菌侵染芒果后叶片细胞壁降解酶活性测定(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第10期
页码:
138-141
栏目:
植物保护
出版日期:
2019-06-12

文章信息/Info

Title:
Study on determination of cell wall degrading enzyme activity in mango leaves infected by leaf blight
作者:
杨郑州 黄柳芳 谢晓娜 苏仕林
百色学院农业与食品工程学院,广西百色 533000
Author(s):
Yang Zhengzhouet al
关键词:
芒果叶疫病细胞壁降解酶活性测定35-二硝基水杨酸法紫外分光光度计法减少产量损失
Keywords:
-
分类号:
S436.67+9
DOI:
-
文献标志码:
A
摘要:
为明确叶疫病病菌侵染芒果后细胞壁降解酶活性的变化,以台农芒果叶作为试验材料,通过体外培养的方法分离得到芒果叶疫病菌,将其接种于离体的芒果幼叶,利用3,5-二硝基水杨酸(DNS)法和紫外分光光度计法检测病原菌侵染过程中细胞壁降解酶活性的变化。结果表明,在细胞壁降解酶中,β-葡萄糖苷酶活性最高,羧甲基纤维素酶(Cx)活性与多聚半乳糖醛酸酶(PG)活性次之,聚甲基半乳糖醛酸酶(PMG)活性最低;纤维素酶在病原菌侵染初期最早分泌并起作用,并且纤维素酶比果胶酶对芒果叶片的致病作用明显。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-01-09
基金项目:国家自然科学基金(编号:31760221);广西自然科学基金(编号:2017GXNSFBA198007);2017年广西高校中青年教师基础能力提升项目(编号:2017KY0742、2017KY0743);2017年百色学院校级特色研究团队项目(编号:百院字〔2017〕235号)。
作者简介:杨郑州(1982—),男,河南平顶山人,硕士,助教,主要从事动植物生理生化方面的研究。E-mail:21775285@qq.com。
通信作者:谢晓娜,博士,讲师,主要从事作物逆境生理及分子的研究。E-mail:452712796@qq.com。
更新日期/Last Update: 2019-05-20