|本期目录/Table of Contents|

[1]宋小飞,芮伟康,陶书田.不同浓度外源钙对梨果实石细胞的影响[J].江苏农业科学,2019,47(14):148-152.
 Song Xiaofei,et al.Impacts of different concentrations of exogenous calcium on stone cells of pear fruit[J].Jiangsu Agricultural Sciences,2019,47(14):148-152.
点击复制

不同浓度外源钙对梨果实石细胞的影响(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第14期
页码:
148-152
栏目:
园艺与林学
出版日期:
2019-08-10

文章信息/Info

Title:
Impacts of different concentrations of exogenous calcium on stone cells of pear fruit
作者:
宋小飞 芮伟康 陶书田
南京农业大学园艺学院,梨工程技术研究中心,江苏南京 210095
Author(s):
Song Xiaofeiet al
关键词:
砀山酥梨石细胞愈伤组织木质素肉桂醇脱氢酶
Keywords:
-
分类号:
S661.201
DOI:
-
文献标志码:
A
摘要:
以田间多年生砀山酥梨果实及其诱导的愈伤组织为试验材料,通过施用不同浓度CaCl2(0、0.25%、0.50%、0.75%),探究抑制石细胞形成的适宜钙浓度。结果表明,一定浓度的钙离子能够降低肉桂醇脱氢酶的活性,抑制木质素的合成,降低石细胞含量,且以0.50% CaCl2效果最佳。不同种类及配比的激素对愈伤组织的诱导及继代培养效果不同,筛选出MS+2.0 mg/L 2,4-D+0.5 mg/L 6-BA+0.5 mg/L NAA为最适的砀山酥梨果实诱导培养基,MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA+1.0 mg/L KT为最适的继代培养基。与田间材料和愈伤组织为研究对象的试验结果显示一致,由此推测外界因素对钙调控木质素合成的影响较小,适宜浓度的CaCl2能够显著降低田间梨果实中的石细胞含量,提高食用品质。
Abstract:
-

参考文献/References:

[1]Wei H,Liu L,Wang M D,et al. Differentially expressed genes related to the formation of russet fruit skin in a mutant of ‘Dangshansuli’ pear(Pyrus bretchnederi Rehd.)determined by suppression subtractive hybridization[J]. Euphytica,2014,196(2):285-297.
[2]徐义流. 砀山酥梨[M]. 北京:中国农业出版社,2009:1-3.
[3]李玲,蔡永萍,刘小阳. 梨果实的石细胞[J]. 植物生理学通讯,2004,40(5):629-632.
[4]刘小阳,李玲,宗梅,等. 梨果实石细胞含量分布及其对梨品质的影响[J]. 安徽农业大学学报,2004,31(1):104-106.
[5]王纪忠,陶书田,齐开杰,等. 喷硼对梨果硼、钙含量及石细胞形成相关酶活性的影响[J]. 植物营养与肥料学报,2011,17(5):1250-1255.
[6]李疆,任莹莹,吐尔逊·阿依,等. 氯化锌对库尔勒香梨果实品质的影响[J]. 经济林研究,2009,27(2):16-19.
[7]Palta J P. Role of Calcium in plant responses to stresses:linking basic research to the solution of practical problems[J]. HortScience,1996,31(1):51-57.
[8]朱晓军,杨劲松,梁永超,等. 盐胁迫下钙对水稻幼苗光合作用及相关生理特性的影响[J]. 中国农业科学,2004,37(10):1497-1503.
[9]孙大业,郭艳林,马力耕. 细胞信号传导[M]. 北京:科学出版社,2001.
[10]王晓佳,贾永华,王春良. 不同钙肥处理对金冠苹果品质的影响[J]. 安徽农业科学,2015,43(35):62-64.
[11]陈桂芬. 施钙肥可提高春甜橘产量和品质[J]. 农家顾问,2014(2):31.
[12]文旭. 不同有机钙肥对库尔勒香梨果实相关品质的影响研究[D]. 乌鲁木齐:新疆农业大学,2012.
[13]Raese J T,Drake S R,Staiff D C. Influence of different Calcium materials and spray timing on mineral composition,yield,fruit quality,and control of fruit disorders of ‘Anjou’ pears[J]. Journal of Plant Nutrition,1995,18(4):823-838.
[14]李中勇,高东升. 喷钙对设施油桃产量和品质的影响[J]. 落叶果树,2007,39(3):25-26.
[15]李丙智,梁俊,张林森,等. CA2000钙宝叶肥在富士苹果树上应用研究[J]. 西北林学院学报,2001,16(3):26-28.
[16]赵娟. 梨果实愈伤组织对致腐真菌侵染的生理响应机制研究[D]. 杨凌:西北农林科技大学,2012.
[17]Syros T,Yupsanis T,Zafiriadis H,et al. Activity and isoforms of peroxidases,lignin and anatomy,during adventitious rooting in cuttings of Ebenus cretica L.[J]. Journal of Plant Physiology,2004,161(1):69-77.
[18]Tao S T,Khanizadeh S,Zhang H,et al. Anatomy,ultrastructure and lignin distribution of stone cells in two Pyrus species[J]. Plant Science,2009,176(3):413-419.
[19]Goffner D,Joffroy I,Grima-Pettenati J.purification and characterization of isoforms of cinnamyl alcohol dehydrogenase from eucalyptus,xylem[J]. Planta,1992,188(1):48-53.
[20]陶书田. 梨(Pyrus)果实石细胞的结构成分分析及相关酶基因的克隆[D]. 南京:南京农业大学,2009.
[21]Wang B,Zhang N,Yan C C,et al. Bagging for the development of stone cell and metabolism of lignin in Pyrus bretschneideri ‘Dangshan Suli’[J]. Acta Horticulturae Sinica,2013,40(3):531-539.
[22]王丹阳. 木质素代谢相关酶在梨石细胞合成中的作用[D]. 南京:南京农业大学,2014.
[23]Ma C,Zhang H P,Li J M,et al. Genome-wide analysis and characterization of molecular evolution of the HCT gene family in pear (Pyrus bretschneideri)[J]. Plant Systematics and Evolution,2017,303(1):71-90.
[24]Cao Y P,Han Y H,Meng D D,et al. Structural,evolutionary,and functional analysis of the class Ⅲ peroxidase gene family in Chinese pear (Pyrus bretschneideri)[J]. Frontiers in Plant Science,2016,7:1874.
[25]Lu G L,Li Z J,Zhang X F,et al. Expression analysis of lignin-associated genes in hard end pear (Pyrus pyrifolia Whangkeumbae) and its response to calcium chloride treatment conditions[J]. Journal of Plant Growth Regulation,2015,34(2):251-262.
[26]Kim S J,Kim K W,Cho M H,et al. Expression of cinnamyl alcohol dehydrogenases and their putative homologues during Arabidopsis thaliana growth and development:lessons for database annotations[J]. Phytochemistry,2007,68(14):1957-1974.

相似文献/References:

[1]赵阅书,薛晓明,王艺蓉.青冈树韧皮部木质部显微构造的比较[J].江苏农业科学,2020,48(18):192.
 Zhao Yueshu,et al.Comparison of microstructure of xylem and phloem of Cyclobalanopsis glauca[J].Jiangsu Agricultural Sciences,2020,48(14):192.

备注/Memo

备注/Memo:
收稿日期:2018-04-26
基金项目:新疆生产建设兵团南疆重点产业创新发展支撑计划(编号:2017DB006)。
作者简介:宋小飞(1992—),男,山东威海人,硕士研究生,主要从事果树生理与分子生物学研究。E-mail:1608299022@qq.com。
通信作者:陶书田,博士,副教授,主要从事果树生理与分子生物学研究。E-mail:taost@njau.edu.cn。
更新日期/Last Update: 2019-07-20