|本期目录/Table of Contents|

[1]武娅歌,赵建华,宋晓飞,等.欧洲温室型黄瓜同源四倍体新种质的创制与鉴定[J].江苏农业科学,2019,47(18):141-145.
 Wu Yage,et al.Creation and identification of new autotetraploid germplasm of European greenhouse cucumber[J].Jiangsu Agricultural Sciences,2019,47(18):141-145.
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欧洲温室型黄瓜同源四倍体新种质的创制与鉴定(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第18期
页码:
141-145
栏目:
园艺与林学
出版日期:
2019-10-15

文章信息/Info

Title:
Creation and identification of new autotetraploid germplasm of European greenhouse cucumber
作者:
武娅歌 赵建华 宋晓飞 李晓丽 孙成振 闫立英 郑金双
河北科技师范学院,河北秦皇岛 066000
Author(s):
Wu Yageet al
关键词:
欧洲温室型黄瓜秋水仙素多倍体二倍体同源四倍体气孔细胞学诱导效果形态特征
Keywords:
-
分类号:
S642.202.4
DOI:
-
文献标志码:
A
摘要:
为构建黄瓜同源四倍体诱导技术体系,创制新种质,以3个秋水仙素浓度(0.1、0.2、0.3 g/L)3个处理次数(3、5、7次)对二倍体欧洲温室型黄瓜(2n=2x=14)幼苗生长点进行处理,通过植株形态鉴定与气孔鉴定、DNA相对含量鉴定和细胞学鉴定等方法,筛选诱变植株并鉴定倍性。研究发现,以2、3 g/L秋水仙素处理5次诱导效果较好,获得了欧洲温室型黄瓜同源四倍体(2n=4x=28)和非整倍体(2n=4x=26)。与二倍体亲本相比,同源四倍体黄瓜子叶大小、叶片大小、主茎粗、雌雄花大小、保卫细胞大小和保卫细胞内叶单位面积绿体数目等均呈极显著增加,但气孔密度极显著减少。本研究获得了欧洲温室型黄瓜同源多倍体新种质,为黄瓜种质创新与欧洲温室型新品种选育奠定了基础。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2018-06-08
基金项目:河北省自然科学基金(编号:C2015407058)。
作者简介:武娅歌(1993—),女,河北邢台人,硕士研究生,研究方向为蔬菜作物育种。E-mail:969361514@qq.com。
通信作者:郑金双,博士,讲师,研究方向为蔬菜作物、药用植物栽培和育种。E-mail:jinshuangk@163.com。
更新日期/Last Update: 2019-09-20