[1]张圣新,朱磊,鲍恩东,等.固相萃取-高效液相色谱法测定养殖污水中强力霉素残留[J].江苏农业科学,2019,47(23):249-251.
Zhang Shengxin,et al.Determination of doxycycline residues in farm wastewater using solid phase extraction and high performance liquid chromatography[J].Jiangsu Agricultural Sciences,2019,47(23):249-251.
点击复制
固相萃取-高效液相色谱法测定养殖污水中强力霉素残留(PDF)
Zhang Shengxin,et al.Determination of doxycycline residues in farm wastewater using solid phase extraction and high performance liquid chromatography[J].Jiangsu Agricultural Sciences,2019,47(23):249-251.
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第47卷
- 期数:
- 2019年第23期
- 页码:
- 249-251
- 栏目:
- 贮藏加工与检测分析
- 出版日期:
- 2019-12-30
文章信息/Info
- Title:
- Determination of doxycycline residues in farm wastewater using solid phase extraction and high performance liquid chromatography
- 作者:
- 张圣新1; 朱磊1; 鲍恩东2; 王冉1; 魏瑞成1
-
1.省部共建国家重点实验室培育基地-江苏省食品质量安全重点实验室/农业农村部农产品质量安全风险
评估实验室(南京)/江苏省农业科学院农产品质量安全与营养研究所，江苏南京 210014； 2.南京农业大学，江苏南京 210095
- Author(s):
- Zhang Shengxin; et al
- Keywords:
- -
- 分类号:
- X839.2
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 为了监测养殖场排污水中兽药抗生素污染状况，拟建立1种固相萃取-高效液相色谱方法测定养殖场污水中强力霉素残留。养殖场排污水离心后取上清液过滤膜并调整溶液pH值，分别采用固相萃取柱进行富集和洗脱，氮吹浓缩处理，C18色谱柱分离，0.01 mol/L乙二酸溶液+甲醇-乙腈溶液洗脱。结果显示，标准曲线在1~20 μg/mL浓度范围内呈线性关系，相关系数r为0.999 8；在10~50 μg/L添加浓度范围内，强力霉素平均回收率为91.55%~9514%，日内相对标准偏差为1.66%~6.45%，日间相对标准偏差为0.62%~0.75%，该方法的检测限为 0.1 μg/mL。该方法操作简便、高效，适用于养殖场排污水中强力霉素残留的快速测定。
- Abstract:
- -
参考文献/References:
［1］Zhang Q Q，Ying G G，Pan C G，et al. Comprehensive evaluation of antibiotics emission and fate in the river basins of China：source analysis，multimedia modeling，and linkage to bacterial resistance［J］. Environmental Science & Technology，2015，49(11)：6772-6782.
［2］Sarmah A K，Meyer M T，Boxall A B A. A global perspective on the use，sales，exposure pathways，occurrence，fate and effects of veterinary antibiotics (VAs) in the environment［J］. Chemosphere，2006，65(5)：725-759.
［3］Daghrir R，Drogui P. Tetracycline antibiotics in the environment：a review［J］. Environmental Chemistry Letters，2013，11(3)：209-227.
［4］刘新程，董元华，王辉. 江苏省集约化养殖畜禽排泄物中四环素类抗生素残留调查［J］. 农业环境科学学报，2008，27(3)：1177-1182.
［5］Batt A L，Bruce I B，Aga D S. Evaluating the vulnerability of surface waters to antibiotic contamination from varying wastewater treatment plant discharges［J］. Environmental Pollution，2006，142(2)：295-302.
［6］Hanna N，Sun P，Sun Q，et al. Presence of antibiotic residues in various environmental compartments of Shandong province in eastern China：its potential for resistance development and ecological and human risk［J］. Environment International，2018，114：131-142.
［7］Xu J，Xu Y，Wang H M，et al. Occurrence of antibiotics and antibiotic resistance genes in a sewage treatment plant and its effluent-receiving river［J］. Chemosphere，2015，119：1379-1385.
［8］Zhao L，Dong Y H，Wang H. Residues of veterinary antibiotics in manures from feedlot livestock in eight provinces of China［J］. Science of the Total Environment，2010，408(5)：1069-1075.
［9］廖治锋，段新华. 兽用多西环素的研究进展［J］. 黑龙江畜牧兽医，2015(5)：76-78.
［10］Peng P C，Wang Y，Liu L Y，et al. The excretion and environmental effects of amoxicillin，ciprofloxacin，and doxycycline residues in layer chicken manure［J］. Poultry Science，2016，95(5)：1033-1041.
［11］Heuer H，Schmitt H，Smalla K. Antibiotic resistance gene spread due to manure application on agricultural fields［J］. Current Opinion in Microbiology，2011，14(3)：236-243.
［12］王瑞，魏源送. 畜禽粪便中残留四环素类抗生素和重金属的污染特征及其控制［J］. 农业环境科学学报，2013，32(9)：1705-1719.
［13］He L Y，Ying G G，Liu Y S，et al. Discharge of swine wastes risks water quality and food safety：antibiotics and antibiotic resistance genes from swine sources to the receiving environments［J］. Environment International，2016，92/93：210-219.
［14］王旭丹，刘静，王庚南，等. 高效液相色谱法检测鸡蛋中四环素类药物残留方法研究［J］. 中国畜牧兽医，2016，43(6)：1579-1584.
［15］贾涛. 液相色谱-串联质谱法检测牛奶中的四环素类药物［J］. 中国乳业，2015(4)：54-58.
［16］王娜妮，寿旦，王绪平，等. 移液枪头式固相微萃取-高效液相色谱法测定细胞培养液中的4种生物碱［J］. 色谱，2017，35(1)：14-19.
［17］魏瑞成，王冉，李维，等. 猪粪中金霉素残留的测定方法［J］. 浙江农业学报，2008，20(4)：291-295.
［18］Wei R C，Ge F，Huang S Y，et al. Occurrence of veterinary antibiotics in animal wastewater and surface water around farms in Jiangsu Province，China［J］. Chemosphere，2011，82(10)：1408-1414.
相似文献/References:
[1]钱宗耀,马磊,安冉,等.气相色谱-质谱联用法检测咖啡中49种农药残留量[J].江苏农业科学,2013,41(07):287.
Qian Zongyao,et al.Detection of 49 pesticides residue in coffee by GC-MS method[J].Jiangsu Agricultural Sciences,2013,41(23):287.
[2]周玮,徐文君,颜春荣,等.全自动固相萃取-气质联用测定酒中氨基甲酸乙酯[J].江苏农业科学,2013,41(07):290.
Zhou Wei,et al.Detection of ethyl carbamate in wine by automatic solid-phase extraction coupled with GC-MS method[J].Jiangsu Agricultural Sciences,2013,41(23):290.
[3]洪华,武中平,王莉,等.固相萃取-GC-MS快速测定杀虫剂产品中17种违禁添加的有机磷类成分[J].江苏农业科学,2014,42(03):244.
Hong Hua,et al.Rapid determination of 17 kinds of contraband organic phosphorus compounds in pesticide products by solid phase extraction-gas chromatography-mass spectrometer method[J].Jiangsu Agricultural Sciences,2014,42(23):244.
[4]林祥群,卢春霞,罗小玲,等.气相色谱-质谱法测定枸杞中28种农药残留[J].江苏农业科学,2016,44(01):285.
Lin Xiangqun,et al.Simultaneous determination of 28 pesticides residues in Chinese wolfberry (Lycium chinense L.) by gas chromatography-mass spectrometry[J].Jiangsu Agricultural Sciences,2016,44(23):285.
[5]黄雪玲,戚隽渊,魏厚道,等.固相萃取-高效液相色谱法同时测定水产品中的己烯雌酚和甲基睾丸酮[J].江苏农业科学,2015,43(05):285.
Huang Xueling,et al.Simultaneous determination of diethylstilbestrol and methyltestosterone in aquatic products by solid phase extraction-high performance liquid chromatography[J].Jiangsu Agricultural Sciences,2015,43(23):285.
[6]万译文,黄向荣,李小玲,等.固相萃取-高效液相色谱法测定渔业水体中16种多环芳烃[J].江苏农业科学,2015,43(01):280.
Wan Yiwen,et al.Determination of 16 kinds of polycyclic aromatic hydrocarbons in fishery water bodies by solid phase extraction-high performance liquid chromatography[J].Jiangsu Agricultural Sciences,2015,43(23):280.
[7]贾林贤,姜蕾,林勇,等.水中壬基酚残留检测方法的研究及其应用[J].江苏农业科学,2017,45(09):147.
Jia Linxian,et al.Research and application of detection methods of nonylphenol residue in water[J].Jiangsu Agricultural Sciences,2017,45(23):147.
[8]董飞,王淑芳,张笑,等.粒径及取样量对小麦中主要镰刀菌毒素定量检测的影响[J].江苏农业科学,2020,48(19):206.
Dong Fei,et al.Impacts of particle size and sampling scale on quantitative determination of major Fusarium mycotoxins in wheats[J].Jiangsu Agricultural Sciences,2020,48(23):206.
[9]许永,黄海涛,李晶,等.带固相萃取功能的索氏提取装置应用于气相色谱-质谱法测定薰衣草中挥发性成分[J].江苏农业科学,2020,48(23):181.
Xu Yong,et al.Soxhlet extraction device with solid phase extraction function applied to determination of volatile components in lavender by gas chromatography-mass spectrometry[J].Jiangsu Agricultural Sciences,2020,48(23):181.
[10]冷远鹏,薛晓康,孙华.固相萃取-高效液相色谱质谱法测定有机肥中18种抗生素残留[J].江苏农业科学,2021,49(11):146.
Leng Yuanpeng,et al.Determination of 18 antibiotics residues in organic fertilizer by solid phase extraction-high performance liquid chromatographymass spectrometry[J].Jiangsu Agricultural Sciences,2021,49(23):146.
备注/Memo
- 备注/Memo:
-
收稿日期：2019-01-24
基金项目：国家农产品质量安全风险评估项目(编号：GJFP2018012、GJFP2019012)。
作者简介：张圣新(1992—)，女，江苏镇江人，硕士，研究实习员，主要从事抗生素安全评价研究。E-mail：17351781405@163.com。
通信作者：魏瑞成，博士，副研究员，主要从事抗生素安全评价研究。E-mail：rcwei79@jaas.ac.cn。
常用功能
统计/Statistics
- 摘要浏览/Viewed822
- 全文下载/Downloads201
- 评论/Comments
