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[1]方明,李洁,赖欣,等.短期生物炭刺激对红壤和潮土微生物群落的影响[J].江苏农业科学,2020,48(11):250-258.
　Fang Ming,et al.Effects of short-term biochar stimulation on microbial communities in red soil and fluvo-aquic soil[J].Jiangsu Agricultural Sciences,2020,48(11):250-258.
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短期生物炭刺激对红壤和潮土微生物群落的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第11期
页码:
250-258
栏目:
资源与环境
出版日期:
2020-06-05

文章信息/Info

Title:
Effects of short-term biochar stimulation on microbial communities in red soil and fluvo-aquic soil
作者:
方明1; 2;  李洁1;  赖欣1;  王知文1; 3;  张贵龙1;  姜淑苓2
1.农业农村部环境保护科研监测所，天津 300191； 2.中国农业科学院果树研究所，辽宁兴城 125100；
3.东北农业大学资源与环境学院，黑龙江哈尔滨 150030
Author(s):
Fang Ming; et al
关键词:
生物炭; 红壤; 潮土; 土壤微生物; PLFAs; 群落结构; 主成分分析
Keywords:
-
分类号:
S154.1
DOI:
-
文献标志码:
A
摘要:
通过施用不同质量比的生物炭(0.5%、1.0%、2.0%、4.0%)，研究生物炭施用对红壤和潮土微生物群落结构的影响。通过土柱培养试验，采用磷脂脂肪酸法测定不同处理土壤微生物的群落结构。添加1.0%、2.0%、4.0%生物炭处理与添加05%生物炭处理相比，潮土细菌PLFAs含量增加了9.5%~56.7%；添加0.5%和1.0%生物炭细菌的磷脂脂肪酸(PLFAs)含量分别显著降低了33.7%、27.3%；添加0.5%、2.0%、4.0%生物炭处理的革兰氏阴性细菌PLFAs的含量与单施氮肥处理相比显著下降了276%~48.8%，生物炭施用对真菌、放线菌、革兰氏阳性细菌、真菌PLFAs含量和细菌PLFAs含量的比值(真/细)和总PLFAs含量总体没有显著影响。生物炭施用对红壤的微生物PLFAs含量没有显著影响。在添加生物炭的处理中，生物炭施用量的增加使饱和脂肪酸含量/不饱和脂肪酸含量有降低的趋势。通过比值分析、多样性分析和主成分分析可知，短期生物炭刺激对红壤和潮土的微生物群落结构没有显著影响，2种土壤微生物生态位未产生分化。
Abstract:
-

参考文献/References:

［1］闵炬，施卫明. 不同施氮量对太湖地区大棚蔬菜产量、氮肥利用率及品质的影响［J］. 植物营养与肥料学报，2009，15(1)：151-157.
［2］陈温福，张伟明，孟军. 农用生物炭研究进展与前景［J］. 中国农业科学，2013，46(16)：3324-3333.
［3］Jones D L，Rousk J，Edwards-Jones G，et al. Biochar-mediated changes in soil quality and plant growth in a three year field trial［J］. Soil Biology and Biochemistry，2012，45：113-124.
［4］Lin Y X，Ding W X，Liu D Y，et al. Wheat straw-derived biochar amendment stimulated N2O emissions from rice paddy soils by regulating the amoA genes of ammonia-oxidizing bacteria［J］. Soil Biology and Biochemistry，2017，113：89-98.
［5］Qin X B，Li Y E，Wang H，et al. Long-term effect of biochar application on yield-scaled greenhouse gas emissions in a rice paddy cropping system：a four-year case study in south China［J］. Science of the Total Environment，2016，569/570：1390-1401.
［6］姚钦. 生物炭施用对东北黑土土壤理化性质和微生物多样性的影响［D］. 北京：中国科学院大学(中国科学院东北地理与农业生态研究所)，2017.
［7］Bamminger C，Zaiser N，Zinsser P，et al. Effects of biochar，earthworms，and litter addition on soil microbial activity and abundance in a tem-perate agricultural soil［J］. Biology and Fertility of Soils，2014，50：1189-1200.
［8］Steinbeiss S，Gleixner G，Antonietti M. Effect of biochar amendment on soil carbon balance and soil microbial activity［J］. Soil Biology and Biochemistry，2009，41(6)：1301-1310.
［9］张梦阳，夏浩，吕波，等. 短期生物炭添加对不同类型土壤细菌和氨氧化微生物的影响［J］. 中国农业科学，2019，52(7)：1260-1271.
［10］Abujabhah I S，，Richard B D，Sally A B，et al. Assessment of bacterial community composition，methanotrophic and nitrogen-cycling bacteria in three soils with different biochar application rates［J］. Journal of Soils and Sediments，2017，18(1)：148-158.
［11］Liu S N，Meng J，Jiang L L，et al. Rice husk biochar impacts soil phosphorous availability，phosphatase activities and bacterial community characteristics in three different soil types［J］. Applied Soil Ecology，2017，116：12-22.
［12］武爱莲，丁玉川，焦晓燕，等. 玉米秸秆生物炭对褐土微生物功能多样性及细菌群落的影响［J］. 中国生态农业学报，2016，24(6)：736-743.
［13］顾美英，葛春辉，马海刚，等. 生物炭对新疆沙土微生物区系及土壤酶活性的影响［J］. 干旱地区农业研究，2016，34(4)：225-230，273.
［14］郑慧芬，吴红慧，翁伯琦，等. 施用生物炭提高酸性红壤茶园土壤的微生物特征及酶活性［J］. 中国土壤与肥料，2019(2)：68-74.
［15］赵兰凤，张新明，程根，等. 生物炭对菜园土壤微生物功能多样性的影响［J］. 生态学报，2017，37(14)：4754-4762.
［16］方明，任天志，赖欣，等. 花生壳生物炭对潮土和红壤理化性质和温室气体排放的影响［J］. 农业环境科学学报，2018，37(6)：1300-1310.
［17］Wu Y P，Na D，Wang W，et al. Effects of different soil weights，storage times and extraction methods on soil phospholipid fatty acid analyses［J］. Geoderma，2009，150(1/2)：171-178.
［18］Frostegrd ，Bth E，Tunlid A. Shift in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis［J］. Soil Biology and Biochemistry，1993，25(6)：723-730.
［19］Prayogo C，Jones J E，Baeyens J，et al. Impact of biochar on mineralisation of C and N from soil and willow litter and its relationship with microbial community biomass and structure［J］. Biology and Fertility of Soils，2014，50(4)：695-702.
［20］Taketani R G，Tsai S M. The Influence of different land uses on the structure of archaeal communities in amazonian anthrosols based on 16S rRNA and amoA Genes［J］. Microbial Ecology，2010，59(4)：734-743.
［21］Harter J，Weigold，P，El-Hadidi M，et al. Soil biochar amendment shapes the composition of N2O-reducing microbial communities［J］. Science of the Total Environment，2016，562：379-390.
［22］袁晶晶，同延安，卢绍辉，等. 生物炭与氮肥配施改善枣区土壤微生物学特性［J］. 植物营养与肥料学报，2018，24(4)：1039-1046.
［23］Luo S S，Wang S J，Tian L，et al. Long-term biochar application influences soil microbial community and its potential roles in semiarid farmland［J］. Applied Soil Ecology，2017，117/118：10-15.
［24］Anderson C R，Condron L M，Clough T J，et al. Biochar induced soil microbial community change：Implications for biogeochemical cycling of carbon，nitrogen and phosphorus［J］. Pedobiologia，2011，54(5/6)：309-320.
［25］Watzinger A，Feichtmair S，Kitzler B，et al. Soil microbial communities responded to biochar application in temperate soils and slowly metabolized 13C-labelled biochar as revealed by 13C PLFA analyses：results from a short-term incubation and pot experiment［J］. European Journal of Soil Science，2014，65(1)：40-51.
［26］He L L，Bi Y C，Zhao J，et al. Population and community structure shifts of ammonia oxidizers after four-year successive biochar application to agricultural acidic and alkaline soils［J］. Science of the Total Environment，2018，619/620：1105-1115.
［27］盖霞普，刘宏斌，翟丽梅，等. 生物炭对中性水稻土养分和微生物群落结构影响的时间尺度变化研究［J］. 农业环境科学学报，2016，35(4)：719-728.
［28］Spokas K A，Baker J M，Reicosky D C. Ethylene：potential key for biochar amendment impacts［J］. Plant and Soil，2010，333(1/2)：443-452.
［29］Thiessen S，Gleixner G，Wutzler T，et al. Both priming and temperature sensitivity of soil organic matter decomposition depend on microbial biomass-An incubation study［J］. Soil Biology and Biochemistry，2013，57：739-748.
［30］Anyika C，Majid Z A，Rashid M，et al. Toxic and nontoxic elemental enrichment in biochar at different production temperatures［J］. Journal of Cleaner Production，2016，131：810-821.
［31］Anderson C R，Hamonts K，Clough T J，et al. Biochar does not affect soil N-transformations or microbial community structure under ruminant urine patches but does alter relative proportions of nitrogen cycling bacteria［J］. Agriculture，Ecosystems & Environment，2014，191：63-72.
［32］Lehmann J，Rill M C，Thies J，et al. Biochar effects on soil biota-a review［J］. Soil Biology and Biochemistry，2011，43(9)：1812-1836.
［33］Steinbeiss S，Gleixner G，Antoniett M. Effect of biochar amendment on soil carbon balance and soil microbial activity［J］. Soil Biology and Biochemistry，2009，41(6)：1301-1310.
［34］程扬，刘子丹，沈启斌，等. 秸秆生物炭施用对玉米根际和非根际土壤微生物群落结构的影响［J］. 生态环境学报，2018，27(10)：1870-1877.
［35］Wang X B，Song D L，Liang G Q，et al. Maize biochar addition rate influences soil enzyme activity and microbial community composition in a fluvo-aquic soil［J］. Applied Soil Ecology，2015，96：265-272.
［36］李明，胡云，黄修梅，等. 生物炭对设施黄瓜根际土壤养分和菌群的影响［J］. 农业机械学报，2016，47(11)：172-178.
［37］Muhammad N，Dai Z M，Xiao K C，et al. Changes in microbial community structure due to biochars generated from different feedstocks and their relationships with soil chemical properties［J］. Geoderma，2014，226(1)：270-278.
［38］Yao M J，Rui J P，Niu H S，et al. The differentiation of soil bacterial communities along a precipitation and temperature gradient in the eastern Inner Mongolia steppe［J］. Catena，2017，152：47-56.
［39］Warnock D D，Lehmann J，Kuyper T W，et al. Mycorrhizal responses to biochar in soil-concepts and mechanisms［J］. Plant Soil，2007，300：9-20.
［40］Farrell M，Kuhn T K，Macdonald L M，et al. Microbial utilisation of biochar-derived carbon［J］. The Science of the Total Environment，2013，465(6)：288-297.
［41］Bamminger C，Zaiser N，Zinsser P，et al. Effects of biochar，earthworms，and litter addition on soil microbial activity and abundance in a tem-perate agricultural soil［J］. Biology and Fertility of Soils，2014，50：1189-1200.
［42］Khadema A，Raiesib F. Responses of microbial performance and community to corn biochar in calcareous sandy and clayey soils［J］. Applied Soil Ecology，2017，114：16-27.
［43］Liu Y J，Mao L，Li J Y，et al. Resource availability differentially drives community assemblages of plants and their root-associated arbuscular mycorrhizal fungi［J］. Plant and Soil，2015，386(1/2)：341-355.
［44］陈坤，徐晓楠，彭靖，等. 生物炭及炭基肥对土壤微生物群落结构的影响［J］. 中国农业科学，2018，51(10)：1920-1930.
［45］朱永琪，董天宇，宋江辉，等. 生物炭影响土壤重金属生物有效性的研究进展［J］. 江苏农业科学，2018，46(16)：9-14.
［46］李兴，勾芒芒，屈忠义，等. 生物炭对沙壤土水分特征的影响［J］. 江苏农业科学，2018，46(12)：280-283.
［47］Rousk J，Bth E，Brookes P C，et al. Soil bacterial and fungal communities across a pH gradient in an arable soil［J］. ISME J，2010，4(10)：1340-1351.

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备注/Memo

备注/Memo:
收稿日期：2019-07-08
基金项目：中国农业科学院科技创新工程。
作者简介：方明(1992—)，男，黑龙江佳木斯人，硕士，主要从事生物炭对土壤碳氮养分循环影响的研究。E-mail：fangmingzhxy@163.com。
通信作者：张贵龙，博士，副研究员，主要从事土壤碳氮循环研究，E-mail：zhangguilong@caas.cn；姜淑苓，硕士，研究员，主要从事果树学研究，E-mail：jshuling@163.com。
更新日期/Last Update: 2020-06-05