|本期目录/Table of Contents|

[1]刘俭俭,张建民,陈万权,等.基于激光共聚焦显微技术鉴别小麦矮腥黑穗病菌和光腥黑穗病菌[J].江苏农业科学,2020,48(16):126-130.
 Liu Jianjian,et al.Detection of teliospores of Tilletia controversa Kühn and Tilletia foetida (Walle.) Lindr based on laser scanning confocal microscope[J].Jiangsu Agricultural Sciences,2020,48(16):126-130.
点击复制

基于激光共聚焦显微技术鉴别小麦矮腥黑穗病菌和光腥黑穗病菌(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第16期
页码:
126-130
栏目:
园艺与林学
出版日期:
2020-08-20

文章信息/Info

Title:
Detection of teliospores of Tilletia controversa Kühn and Tilletia foetida (Walle.) Lindr based on laser scanning confocal microscope
作者:
刘俭俭12 张建民1 陈万权2 刘太国2 高利2
1.长江大学农学院,湖北荆州434025; 2.中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京 100193
Author(s):
Liu Jianjianet al
关键词:
小麦矮腥黑穗病菌小麦光腥黑穗病菌激光共聚焦显微技术检测方法
Keywords:
-
分类号:
S435.121.4+4
DOI:
-
文献标志码:
A
摘要:
由小麦矮腥黑穗病菌(Tilletia controversa Kühn)引起的小麦矮腥黑穗病和由小麦光腥黑穗病菌[Tilletia foetida (Walle.) Lindr]引起的小麦光腥黑穗病都是世界性的病害,小麦矮腥黑穗病是一种重要的国际检疫性病害,是麦类黑穗病中危害最大、极难防治的检疫性病害之一,小麦光星黑穗病在我国被列为北京市的检疫对象。采用激光共聚焦显微技术结合染色方法可简便快速区分小麦矮腥黑穗病菌和小麦光腥黑穗病菌,2种病原菌的冬孢子壁分别呈绿色网纹荧光及均匀平滑绿色荧光,检测可在5~7 min完成,为快速区分2种病原菌的冬孢子提供了一种新的方法。
Abstract:
-

参考文献/References:

[1]Hoffman J A. Bunt of wheat[J]. Plant Disease,1982,66(11):979-986.
[2]Mathre D E. Dwarf bunt:politics,identification,and biology[J]. Annual Review of Phytopathology,1996,34(1):67-85.
[3]李健强,刘洪斌,刘西莉,等. 5种药剂对小麦光腥黑穗病菌的毒力和孢子萌发形态结构的影响[J]. 农药学学报,2000,2(1):41-46.
[4]Pady S M,Duran R,Fischer G W. The genus tilletia[J]. Mycologia,1961,53(2):213.
[5]Young P A. A new variety of Tilletia tritici in Montana[J]. Phytopathology,1935,25:40.
[6]Wilcoxson R D,Saari E E. Bunt and smut diseases of wheat:concepts and methods of disease management[M]. Mexico:CIMMYT,1996:16.
[7]Meiners J P,Waldher J T. Factors affecting spore germination of twelve species of Tilletia from cereals and grasses[J]. Phytopathology,1959,49(11):724-728.
[8]Stockwell V O. Distinguishing teliospores of Tilletia controversa from those of T. cariesby fluorescence microscopy[J]. Plant Disease,1986,70(10):924-926.
[9]蔚慧欣,高利,康晓慧,等. 应用激光共聚焦显微扫描技术鉴别小麦矮腥黑穗病病菌和小麦光腥黑穗病病菌冬孢子[J]. 中国植保导刊,2016,36(2):5-8.
[10]Qing Y,Nian S,Yin Y P,et al. Development of a PCR-based diagnostic tool specific to wheat dwarf bunt,caused by Tilletia controversa[J]. European Journal of Plant Pathology,2009,124(4):585-594.
[11]Liu J H,Gao L,Liu T G,et al. Development of a sequence-characterized amplified region marker for diagnosis of dwarf bunt of wheat and detection of Tilletia controversa Kühn[J]. Letters in Applied Microbiology,2009,49(2):235-240.
[12]Zupunski V,Ignjatovic-Micic D,Nikolic A,et al. Identification of Tilletia species using rep-PCR fingerprinting technique[J]. Genetika,2011,43(1):183-195.
[13]Li G,Yu H X,Han W S,et al. Development of a SCAR marker for molecular detection and diagnosis of Tilletia controversa Kühn,the causal fungus of wheat dwarf bunt[J]. World Journal of Microbiology and Biotechnology,2014,30(12):3185-3195.
[14]Gao L,Chen W Q,Liu T G. An ISSR-based approach for the molecular detection and diagnosis of dwarf bunt of wheat,caused by Tilletia controversa Kühn[J]. Journal of Phytopathology,2011,159(3):155-158.
[15]Chuong S D X,Franceschi V R,Edwards G E. The cytoskeleton maintains organelle partitioning required for single-cell C4 photosynthesis in Chenopodiaceae species[J]. The Plant Cell,2006,18(9):2207-2223.
[16]肖艳梅,付道林,李安生. 激光扫描共聚焦显微镜(LSCM)及其生物学应用[J]. 激光生物学报,1999,8(4):305-311.
[17]李林光,王颖,王玉霞,等. 苹果四倍体品种天星的花粉形态及胚囊发育特征研究[J]. 植物遗传资源学报,2011,12(4):662-666.
[18]任宏,刘永胜,孙敬三. 用共聚焦扫描显微镜检术观察水稻胚囊发育[J]. 植物学报,1998,40(9):786.
[19]常慧萍,马忠友,唐欣昀. 麦胚凝集素的纯化及其与小麦根际促生细菌的亲和作用[J]. 中国粮油学报,2009,24(11):26-29.
[20]Gao L,Kelliher T,Nguyen L,et al. Ustilago maydis reprograms cell proliferation in maize anthers[J]. The Plant Journal,2013,75(6):903-914.
[21]Wei X N,Shan T L,Hong Y T,et al. TaPIMP2,a pathogen-induced MYB protein in wheat,contributes to host resistance to common root rot caused by Bipolaris sorokiniana[J]. Scientific Reports,2017,7(1):1-15.
[22]Deshmukh S,Hueckelhoven R,Schaefer P A,et al. The root endophytic fungus Piriformospora indica requires host cell death for proliferation during mutualistic symbiosis with barley[J]. Proceedings of the National Academy of Sciences,2006,103(49):18450-18457.
[23]Paddock S W,Eliceiri K W. Laser scanning confocal microscopy:history,applications,and related optical sectioning techniques[J]. Methods of Molecular Biology,2014,1075:9-47.
[24]蔚慧欣,高利,沈慧敏,等. 小麦矮腥黑粉菌在小麦体内侵染过程的显微观察[J]. 中国科学(生命科学),2016,46(5):637-645.
[25]尚忠林. 共聚焦显微技术简介[J]. 生物学通报,2001,36(12):33-35.
[26]赵启韬,苗俊英. 激光共聚焦显微镜在生物医学研究中的应用[J]. 北京生物医学工程,2003,22(1):52-54.

相似文献/References:

备注/Memo

备注/Memo:
收稿日期:2020-04-29
基金项目:国家重点研发计划(编号:2018YFD0200406);国家自然科学基金国际(地区)合作与交流项目(编号:31761143011)。
作者简介:刘俭俭(1993—),女,河南商丘人,硕士研究生,主要从事小麦腥黑粉菌研究。E-mail:1343725104@qq.com。
通信作者:张建民,博士,教授,主要从事昆虫分子生物学研究。E-mail:371995966@qq.com。
更新日期/Last Update: 2020-08-20