|本期目录/Table of Contents|

[1]沈红梅,程玉祥.杨树PtrRHH94突变体创制及功能初步分析[J].江苏农业科学,2020,48(22):44-49.
　Shen Hongmei,et al.Mutant production and functional analysis of PtrRHH94 gene in populus[J].Jiangsu Agricultural Sciences,2020,48(22):44-49.
点击复制

杨树PtrRHH94突变体创制及功能初步分析(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第22期
页码:
44-49
栏目:
生物技术
出版日期:
2020-11-20

文章信息/Info

Title:
Mutant production and functional analysis of PtrRHH94 gene in populus
作者:
沈红梅;  程玉祥
东北林业大学林木遗传育种国家重点实验室，黑龙江哈尔滨 150040
Author(s):
Shen Hongmei; et al
关键词:
毛果杨; PtrRHH94; 突变体; Cas9/gRNA编辑载体
Keywords:
-
分类号:
S792.110.1
DOI:
-
文献标志码:
A
摘要:
RING-H2家族基因参与植物的多个生物学过程，PtrRHH94是毛果杨木质化组织内高丰度表达的RING-H2基因。为了探究PtrRHH94基因的功能，选择3组PtrRHH94待编辑靶位点，构建相应的Cas9/gRNA编辑载体，将其遗传转化毛果杨，得到10株转基因幼苗。DNA测序结果显示，7株转基因幼苗体内的PtrRHH94位点被编辑突变，3株植株被鉴定为PtrRHH94敲除突变体。与野生型毛果杨幼树相比，PtrRHH94突变体幼树株高增加，茎节数也增多。此外，PtrRHH94突变体木材的纤维长度与野生型相比没有显著差异，表明PtrRHH94参与了毛果杨茎生长的调控。
Abstract:
-

参考文献/References:

［1］Borden K L. RING domains：master builders of molecular scaffolds？［J］. Journal of Molecular Biology，2000，295(5)：1103-1112.
［2］Li Y Z，Wu B J，Yu Y L，et al. Genome-wide analysis of the RING finger gene family in apple［J］. Molecular Genetics and Genomics，2011，286(1)：81-94.
［3］Lim S D，Yim W C，Moon J C，et al. A gene family encoding RING finger proteins in rice：their expansion，expression diversity，and co-expressed genes［J］. Plant Molecular Biology，2010，72(4/5)：369-380.
［4］Stone S L，Hauksdóttir H，Troy A，et al. Functional analysis of the RING-type ubiquitin ligase family of Arabidopsis［J］. Plant Physiology，2005，137(1)：13-30.
［5］Ramadan A，Nemoto K，Seki M，et al. Wheat germ-based protein libraries for the functional characterization of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase enzymes［J］. BMC Plant Biology，2015，15(1)：275.
［6］Lyzenga W J，Booth J K，Stone S L. The Arabidopsis RING-type E3 ligase XBAT32 mediates the proteasomal degradation of the ethylene biosynthetic enzyme，1-aminocyclopropane-1-carboxylate synthase 7［J］. The Plant Journal，2012，71 (1)：23-34.
［7］Thines B，Katsir L，Melotto M，et al. JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signaling［J］. Nature，2007，448(7154)：661-665.
［8］Guo H，Ecker J R. Plant responses to ethylene gas are mediated by SCF (EBF1/EBF2) dependent proteolysis of EIN3 transcription factor［J］. Cell，2003，115(6)：667-677.
［9］Griffiths J，Murase K，Rieu I，et al. Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis［J］. The Plant Cell，2006，18(12)：3399-3414.
［10］Roberts D，Pedmale U V，Morrow J，et al. Modulation of phototropic responsiveness in Arabidopsis through ubiquitination of phototropin 1 by the CUL3-Ring E3 ubiquitin ligase CRL3(NPH3)［J］. The Plant Cell，2011，23 (10)：3627-3640.
［11］Cheng M C，Hsieh E J，Chen J H，et al. Arabidopsis RGLG2，functioning as a RING E3 ligase，interacts with AtERF53 and negatively regulates the plant drought stress response［J］. Plant Physiology， 2012，158(1)：363-375.
［12］Cho S K，Ryu M Y，Seo D H，et al. The Arabidopsis RING E3 ubiquitin ligase AtAIRP2 plays combinatory roles with AtAIRP1 in abscisic acid-mediated drought stress responses［J］. Plant Physiology，2011，157(4)：2240-2257.
［13］Gao T，Wu Y R，Zhang Y Y，et al. OsSDIR1 overexpression greatly improves drought tolerance in transgenic rice［J］. Plant Molecular Biology，2011，76(1/2)：145-156.
［14］Tong G M，Shen H M，Cao S Q，et al. Identification of RING-H2 gene candidates related to wood formation in poplar［J］. Forests，2019，10(8)：698.
［15］Xing H L，Dong L，Wang Z P，et al. A CRISPR/Cas9 toolkit for multiplex genome editing in plants［J］. BMC Plant Biology，2014，14(1)：327.
［16］Li S J，Zhen C，Xu W J，et al. Simple，rapid and efficient transformation of genotype Nisqually-1：a basic tool for the first sequenced model tree［J］. Scientific Reports，2017，7(1)：2638.
［17］Tuskan G A，Difazio S，Jansson S，et al. The genome of black cottonwood，Populus trichocarpa (Torr.＆ Gray)［J］. Science，2006，313(5793)：1596-1604.
［18］Feng Q，Yoh S，Tran L S，et al. Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought stress-responsive gene expression［J］. The Plant Cell，2008，20(6)：1693-1707.
［19］Liu L F，Jin L，Huang X H，et al. OsRFPH2-10，a ring-H2 finger E3 ubiquitin ligase，is involved in rice antiviral defense in the early stages of rice dwarf virus infection［J］. Molecular Plant，2014，7(6)：1057-1060.

相似文献/References:

[1]奈婕菲,程玉祥.一个杨树GDSL基因组织表达的特性及其在拟南芥异源的表达[J].江苏农业科学,2014,42(03):16.
　Nai Jiefei,et al.Tissue expression of a poplar GDSL gene and its heterologous expression analysis in Arabidopsis thaliana[J].Jiangsu Agricultural Sciences,2014,42(22):16.
[2]毕田田,张骐,代金玲,等.毛果杨PtIPT5基因的克隆及低温胁迫表达分析[J].江苏农业科学,2023,51(22):14.
　Bi Tiantian,et al.Cloning of PtIPT5 gene in Populus trichocarpa and its expression analysis under low temperature stress[J].Jiangsu Agricultural Sciences,2023,51(22):14.

备注/Memo

备注/Memo:
收稿日期：2020-03-10
基金项目：国家自然科学基金面上项目(编号：31770637)。
作者简介：沈红梅(1994—)，女，四川广安人，硕士研究生，主要从事林木遗传与育种研究。E-mail：shenhm@nefu.edu.cn。
通信作者：程玉祥，博士，教授，主要从事林木遗传与育种研究。E-mail：chengyuxiang@nefu.edu.cn。
更新日期/Last Update: 2020-11-20