|本期目录/Table of Contents|

[1]陈微,惠林冲,李威亚,等.洋葱花青苷合成相关基因表达与不同层鳞片颜色的关系[J].江苏农业科学,2021,49(21):54-57.
 Chen Wei,et al.Relationship between expression of genes related to anthocyanin synthesis in onion and color of different layers of scales[J].Jiangsu Agricultural Sciences,2021,49(21):54-57.
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洋葱花青苷合成相关基因表达与不同层鳞片颜色的关系(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第21期
页码:
54-57
栏目:
生物技术
出版日期:
2021-11-05

文章信息/Info

Title:
Relationship between expression of genes related to anthocyanin synthesis in onion and color of different layers of scales
作者:
陈微1 惠林冲1 李威亚1 潘美红1 郇国磊2 何林玉1 缪美华1 杨海峰1
1.江苏省连云港市农业科学院,江苏连云港 222000; 2.江苏省灌南县农业农村局,江苏灌南 222000
Author(s):
Chen Weiet al
关键词:
洋葱鳞片花青苷相关基因基因表达
Keywords:
-
分类号:
S633.201
DOI:
-
文献标志码:
A
摘要:
为了探讨洋葱不同层肉质鳞片花青苷含量的区别,以及花青苷合成相关基因与含量之间的关系,采用高效液相色谱(HPLC)和实时荧光定量PCR技术,分析了不同层鳞片花青苷含量,以及与花青苷合成相关基因的差异表达情况。结果显示,花青苷含量与洋葱鳞片颜色深浅呈正相关,洋葱鳞片颜色越深,花青苷含量就越高。第2层花青苷含量最高,第4层和第6层含量差异不明显。在第2层鳞片中,苯丙氨酸解氨酶基因(PAL)、查耳酮合成酶基因(CHS)和查耳酮异构酶基因(CHI)的相对表达量较高,与花青苷的含量表现一致。黄烷酮-3-羟化酶基因(F3H)、二氢黄酮醇还原酶基因(DFR)、花青素合成酶基因(ANS)和类黄酮-3-O-糖基转移酶基因(UFGT)的表达量在第6层鳞片中较高,这与后期洋葱内部鳞片颜色逐渐加深相一致。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2021-05-17
基金项目:江苏省农业科技自主创新资金[编号:CX(20)3164];连云港市财政专项(编号:QNJJ1904)。
作者简介:陈微(1990—),女,山东菏泽人,硕士,助理研究员,主要从事蔬菜育种和栽培研究。E-mail:1135050599@qq.com。
通信作者:杨海峰,副研究员,主要从事蔬菜育种和栽培研究。E-mail:13775492959@163.com。
更新日期/Last Update: 2021-11-05