[1]方亦午,顾建锋,熊武,等.基于18S、28S宏条形码高通量测序和形态学方法的土壤线虫群落多样性比较[J].江苏农业科学,2022,50(6):202-210.
Fang Yiwu,et al.Comparison of soil nematode community diversity based on 18S and 28S metabarcoding high-throughput sequencing and morphological methods[J].Jiangsu Agricultural Sciences,2022,50(6):202-210.
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基于18S、28S宏条形码高通量测序和形态学方法的土壤线虫群落多样性比较(PDF)
Fang Yiwu,et al.Comparison of soil nematode community diversity based on 18S and 28S metabarcoding high-throughput sequencing and morphological methods[J].Jiangsu Agricultural Sciences,2022,50(6):202-210.
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第50卷
- 期数:
- 2022年第6期
- 页码:
- 202-210
- 栏目:
- 资源与环境
- 出版日期:
- 2022-03-20
文章信息/Info
- Title:
- Comparison of soil nematode community diversity based on 18S and 28S metabarcoding high-throughput sequencing and morphological methods
- 作者:
- 方亦午1; 顾建锋1; 熊武2; 马欣欣1; 吕晓玲1; 陈先锋1
- 1.宁波海关技术中心/宁波检验检疫科学技术研究院，浙江宁波 315100； 2. 江苏省固体有机废弃物资源化高技术研究重点实验室/国家有机类肥料工程技术研究中心/江苏省有机固体废弃物资源化协同创新中心/南京农业大学资源与环境科学学院，江苏南京 210095
- Keywords:
- -
- 分类号:
- S432.4+5
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 为探究不同靶标位点的高通量测序方法以及形态学鉴定方法对土壤线虫群落分析的影响，选取线虫群落复杂程度高的日本进境罗汉松根际土壤样品，设置18S、28S、形态学鉴定3个处理，比较不同线虫群落分析方法对土壤线虫群落分析的差异。结果表明，线虫群落中属的数量分析，18S组、28S组高于形态学鉴定组；除捕食和杂食性线虫的相对丰度在3组间无显著性差异外，食细菌线虫、食真菌线虫以及植物寄生线虫的相对丰度在3组间均有一定差异。各线虫指数中，除多样性指数在在3组间无显著性差异外，均匀度指数、线虫成熟度指数、植物线虫指数、富集指数、结构指数、基础指数、线虫通道比率在3组间均有较大差异。高通量测序方法形态学鉴定结果存在一定的相关性，结合多基因位点的宏条形码高通测序方法以及形态学鉴定方法，可作为研究土壤线虫群落组分的新策略。
- Abstract:
- -
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备注/Memo
- 备注/Memo:
-
收稿日期：2021-05-24
基金项目：浙江省自然科学基金(编号：LY19C140003)；宁波市自然科学基金 (编号：2019A610405)。
作者简介：方亦午(1990—)，男，浙江舟山人，博士，农艺师，主要从事线虫研究。E-mail： fywsdtcm@126.com。
通信作者：顾建锋，博士，研究员，主要从事线虫研究。E-mail： jeffgu00@qq.com。
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