|本期目录/Table of Contents|

[1]黄启超,顾琼楠,褚世海,等.稗18-WJJ-Ec种群对五氟磺草胺的靶标抗性机制[J].江苏农业科学,2022,50(19):104-110.
 Huang Qichao,et al.Study on target-site resistance mechanism of Echinochloa crusgalli 18-WJJ-Ec to penoxsulam[J].Jiangsu Agricultural Sciences,2022,50(19):104-110.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第19期
页码:
104-110
栏目:
植物保护
出版日期:
2022-10-05

文章信息/Info

Title:
Study on target-site resistance mechanism of Echinochloa crusgalli 18-WJJ-Ec to penoxsulam
作者:
黄启超12顾琼楠2褚世海2陈安安2李林2李儒海2孙正祥1
1.长江大学农学院,湖北荆州 434025; 2.湖北省农业科学院植保土肥研究所/农业农村部华中作物有害生物综合治理重点实验室/农作物重大病虫草害防控湖北省重点实验室,湖北武汉 430064
Author(s):
Huang Qichaoet al
关键词:
五氟磺草胺乙酰乳酸合成酶抗性水平靶标抗性机制
Keywords:
-
分类号:
S451.2
DOI:
-
文献标志码:
A
摘要:
为明确稗(Echinochloa crus-galli)18-WJJ-Ec种群对五氟磺草胺的靶标抗性机制,采用整株生物测定法,以敏感稗18-NJ作为对照,明确稗18-WJJ-Ec种群的抗性指数(RI),通过分子生物学方法测定分析了稗乙酰乳酸合成酶(ALS)基因序列及表达量,并测定稗ALS离体活性。结果表明,五氟磺草胺对稗18-WJJ-Ec种群的鲜质量抑制中浓度(ED50)为20.54 g a.i./hm2,是敏感稗18-NJ种群RI的42.79倍;稗18-WJJ-Ec 的ALS氨基酸基因序列574位色氨酸(Trp)突变成了亮氨酸(Leu);ALS离体活性显著高于敏感稗18-NJ;五氟磺草胺处理1 d后,稗18-WJJ-Ec种群ALS基因表达量极显著高于敏感稗18-NJ种群,ALS基因表达量是对照的8.72倍,总体结果表明稗 18-WJJ-Ec 种群对五氟磺草胺存在靶标抗性机制。
Abstract:
-

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相似文献/References:

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 Liu Xinglin,et al.Control effect of penoxsulam 0.025% GR mixed with fertilizer on annual weeds in transplanted rice fields[J].Jiangsu Agricultural Sciences,2015,43(19):119.
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 Gu Qiongnan,et al.Resistance of Echinochloa spp. weed population to penoxsulam in rice fields of Hubei Province[J].Jiangsu Agricultural Sciences,2023,51(19):105.

备注/Memo

备注/Memo:
收稿日期:2022-05-19
基金项目:湖北省农业科学院领军人才培养计划(编号:L2018012);湖北省农业科学院青年基金(编号:2020NKYJJ09);湖北省农业科技创新中心创新团队项目(编号:2021-620-000-001-13);湖北省农业科学院植保土肥研究所青年基金(编号:2021ZTSQJ04)。
作者简介:黄启超(1991—),男,湖北随州人,硕士,主要从事杂草抗药性和杂草生物防治研究。E-mail:qichao.huang@foxmail.com。
通信作者:孙正祥,博士,副教授,主要从事植物病害生物防治研究,E-mail:sunzhengxiang9904@126.com;李儒海,博士,研究员,主要从事杂草生物生态学及综合治理研究,E-mail:ruhaili73@163.com。
更新日期/Last Update: 2022-10-05