|本期目录/Table of Contents|

[1]马军韬,张国民,邓凌韦,等.水稻纹枯病离体接种鉴定技术因子量化试验[J].江苏农业科学,2022,50(21):127-135.
 Ma Juntao,et al.Quantitative test of technical factors for identification of rice sheath blight inoculation in vitro[J].Jiangsu Agricultural Sciences,2022,50(21):127-135.
点击复制

水稻纹枯病离体接种鉴定技术因子量化试验(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第21期
页码:
127-135
栏目:
植物保护
出版日期:
2022-11-05

文章信息/Info

Title:
Quantitative test of technical factors for identification of rice sheath blight inoculation in vitro
作者:
马军韬1张国民1邓凌韦1王永力1高洪儒1王南博1肖明纲1赵北平1任洋1宫秀杰2
1.黑龙江省农业科学院生物技术研究所,黑龙江哈尔滨 150028; 2.黑龙江省农业科学院耕作栽培研究所,黑龙江哈尔滨 150028
Author(s):
Ma Juntaoet al
关键词:
水稻纹枯病离体叶片接种接种体类型
Keywords:
-
分类号:
S435.111.4+2
DOI:
-
文献标志码:
A
摘要:
为建立精准的水稻纹枯病离体叶片接种鉴定技术,在实验室内完成接种鉴定相关技术因子的量化试验。结果表明,接种后72 h发病基本完全,可以进行数据调查;以菌丝块为接种体,供试水稻发病速度适中,品种间抗感性差别明显;接种叶片长度为30 cm,各水稻品种病斑面积占比介于13.78%~22.56%,可以满足病斑扩展需求;叶片背面接种发病偏慢,仅为同期正面接种病斑面积的24.28%~32.09%;接种温度30 ℃最利于发病,病斑面积占比介于1398%~22.55%;接种相对湿度85%最利于发病,病斑面积占比介于13.91%~23.07%;接种光照时间14 h最利于发病,病斑面积占比介于13.52%~22.97%。此外,本文还应用田间嵌入接种鉴定技术对供试水稻品种的抗性进行了复检,二者的试验结论趋势一致。
Abstract:
-

参考文献/References:

[1]Groth D E. Effects of cultivar resistance and single fungicide application on rice sheath blight,yield,and quality[J]. Crop Protection,2008,27(7):1125-1130.
[2]左示敏,张亚芳,陈宗祥,等. 水稻抗纹枯病遗传育种研究进展[J]. 中国科学(生命科学),2010,40(11):1014-1023.
[3]González-Vera A D,Bernardes-de-Assis J,Zala M,et al. Divergence between sympatric rice-and maize-infecting populations of Rhizoctonia solani AG-1 IA from Latin America[J]. Phytopathology,2010,100(2):172-182.
[4]Ciampi M B,Meyer M C,Costa M J N,et al. Genetic structure of populations of,Rhizoctonia solani,anastomosis Group-1 IA from soybean in Brazil[J]. Phytopathology,2008,98(8):932-941.
[5]谢剑波,秦梦圆,石杨,等. 水稻纹枯病内生拮抗菌的分离鉴定及其生防作用[J]. 湖南农业科学,2019(4):73-75.
[6]韩贵清. 中国寒地耕稻[M]. 北京:中国农业出版社,2011.
[7]Pinson S R M,Capdevielle F M,Oard J H. Confirming QTLs and finding additional loci conditioning sheath blight resistance in rice using recombinant inbred lines[J]. Crop Science,2005,45(2):503-510.
[8]王道泽,洪文英,胡选祥,等. 水稻主栽品种病虫侵害风险及田间抗性综合评价[J]. 农学学报,2014,4(11):26-33.
[9]熊延文,罗道宏. 广德县水稻主栽品种纹枯病及穗腐病抗性试验[J]. 安徽农学通报,2015,21(5):78.
[10]于艳敏,来永才,闫平,等. 黑龙江省第一积温带水稻对纹枯病的抗性分析[J]. 农学学报,2019,9(2):7-10.
[11]Rush M C,Hoff J J,Mcllrath W O. A uniform disease rating system for rice disease in the United States[G]. Louisana:Proc 16th Rice Tech Working Group,1976:64.
[12]过崇俭,陈志谊,王法明. 水稻纹枯病菌Thanatephorus cucumeris (Frank) Donk致病力分化及品种抗性鉴定技术的研究[J]. 中国农业科学,1985,18(5):50-57.
[13]Wasano K,Oro S,Kido Y. The syringe inoculation method for selecting rice plants resistant to sheath blight,Rhizoctonia solani kühn[J]. Japanese Journal of Tropical Agriculture,1983,27(3):131-134.
[14]Eizenga G C,Lee F N,Rutger J N.Screening Oryza species plants for rice sheath blight resistance[J]. Plant Disease,2002,86(7):808-812.
[15]潘学彪,陈宗祥,徐敬友,等. 不同接种调查方法对抗水稻纹枯病遗传研究的影响[J]. 江苏农学院学报,1997,18(3):27-32.
[16]王子斌,左示敏,李刚,等. 水稻成株期对纹枯病的抗性表现研究[J]. 吉林农业大学学报,2011,33(2):144-150,157.
[17]王爱民,许明,陈宗祥,等. 2007—2013年江苏省审定的水稻品种纹枯病抗性分析及抗纹枯病育种策略[J]. 扬州大学学报(农业与生命科学版),2014,35(4):63-67.
[18]罗霄凤,张重梅,张梅,等. 四川水稻品种对纹枯病抗性的评价[J]. 西南农业学报,2016,29(6):1315-1322.
[19]Singh A,Rohilla R,Singh U S,et al. An improved inoculation technique for sheath blight of rice caused by Rhizoctonia solani[J]. Canadian Journal of Plant Pathology,2002,24(1):65-68.
[20]Jia Y,Correa-Victoria F,McClung A,et al. Rapid determination of rice cultivar responses to the sheath blight pathogen Rhizoctonia solani using a micro-chamber screening method[J]. Plant Disease,2007,91(5):485-489.
[21]Correa-Victoria F,Jia Y,Correl J,et al. Sheath blight screening of two breeding populations at CIAT[J]. Rice CAP News,2007,3(8):2-5.
[22]徐国娟,袁正杰,左示敏,等. 水稻苗期纹枯病抗性鉴定微室接种技术的改良[J]. 中国水稻科学,2015,29(1):97-105.
[23]王子斌,左示敏,李刚,等. 水稻抗纹枯病苗期快速鉴定技术研究[J]. 植物病理学报,2009,39(2):174-182.
[24]易润华,朱西儒,周而勋. 水稻纹枯病菌人工接种方法的研究[J]. 广州大学学报(自然科学版),2003,2(3):224-227.
[25]Nelson J C,Oard J H,Groth D,et al. Sheath-blight resistance QTLS in Japonica rice germplasm[J]. Euphytica,2012,184(1):23-34.
[26]郭田,王刘庆,廖美德.PS04菌株对水稻纹枯病的防效及对水稻2种防御性酶活性的诱导[J]. 西北农林科技大学学报(自然科学版),2013,41(6):98-102.
[27]Jia Y,Singh P,Eizenga G C,et al. In vitro identification of cultivar responses to rice sheath blight pathogen Rhizoctonia solani[M]//Norman R J,Meullenet J F. BR Wells Rice research Studies. Arkansas:Univ Arkansas Agric Exp Stn Ser,2002,504:229-236.
[28]曲海艳,袁正杰,潘龙玉,等. 水稻离体叶片纹枯病接种样品菌量检测方法研究[J]. 浙江农业学报,2018,30(10):1686-1693.
[29]杨晓贺,魏松红,顾鑫,等. 东北地区水稻种质资源抗纹枯病研究初报[J]. 植物保护,2020,46(6):205-208,212.
[30]马晨燕,袁正杰,杨海河,等. 水稻离体叶片抗纹枯病接种方法的研究[J]. 浙江农业学报,2016,28(10):1730-1737.
[31]贺闽,尹俊杰,冯志明,等. 水稻稻瘟病和纹枯病抗性鉴定方法[J]. 植物学报,2020,55(5):577-587.
[32]王妍,魏松红,王小皙,等. 水稻主栽品种对纹枯病的抗性鉴定及评价[J]. 江苏农业科学,2020,48(13):125-128.
[33]左示敏,张亚芳,殷跃军,等. 田间水稻纹枯病抗性鉴定体系的确立与完善[J]. 扬州大学学报(农业与生命科学版),2006,27(4):57-61,封3.

相似文献/References:

[1]马旭俊,刘春娟,吕世博,等.绿色荧光蛋白基因在水稻遗传转化中的应用[J].江苏农业科学,2013,41(04):35.
[2]李岳峰,居立海,张来运,等.水分胁迫下丛枝菌根对水稻/绿豆间作系统 作物生长和氮磷吸收的影响[J].江苏农业科学,2013,41(04):58.
[3]崔月峰,孙国才,王桂艳,等.不同施氮水平和前氮后移措施对水稻产量 及氮素利用率的影响[J].江苏农业科学,2013,41(04):66.
[4]张其蓉,宋发菊,田进山,等.长江中下游稻区水稻区域试验品种抗稻瘟病鉴定与评价[J].江苏农业科学,2013,41(04):92.
[5]王麒,张小明,卞景阳,等.不同插秧密度对黑龙江省第二积温带水稻产量及产量构成的影响[J].江苏农业科学,2013,41(05):60.
 Wang Qi,et al.Effect of different transplanting density on yield and yield component of rice in second temperature zone of Heilongjiang Province[J].Jiangsu Agricultural Sciences,2013,41(21):60.
[6]张国良,张森林,丁秀文,等.基质厚度和含水量对水稻育秧的影响[J].江苏农业科学,2013,41(05):62.
 Zhang Guoliang,et al.Effects of substrate thickness and water content on growth of rice seedlings[J].Jiangsu Agricultural Sciences,2013,41(21):62.
[7]赵忠宝,朱清海.稻-蟹-鳅生态系统的能值分析[J].江苏农业科学,2013,41(05):349.
 Zhao Zhongbao,et al.Emergy analysis of paddy-crab-loach ecosystem[J].Jiangsu Agricultural Sciences,2013,41(21):349.
[8]刘嘉德,孙雪梅,易红娟.江苏沿江地区不同水稻品种对纹枯病的敏感差异性[J].江苏农业科学,2014,42(12):185.
 Liu Jiade,et al.Sensitivity difference of different rice varieties against rice sheath blight disease along the Yangtze River in Jiangsu area[J].Jiangsu Agricultural Sciences,2014,42(21):185.
[9]杨红福,姚克兵,束兆林,等.甲氧基丙烯酸酯类杀菌剂对水稻恶苗病的田间药效[J].江苏农业科学,2014,42(12):166.
 Yang Hongfu,et al.Field efficacy of strobilurin fungicides against rice bakanae disease[J].Jiangsu Agricultural Sciences,2014,42(21):166.
[10]唐成,陈露,安敏敏,等.稻瘟病诱导水稻幼苗叶片氧化还原系统的特征谱变化[J].江苏农业科学,2014,42(12):141.
 Tang Cheng,et al.Characteristic spectral changes of redox homeostasis system in rice seedling leaves induced by rice blast[J].Jiangsu Agricultural Sciences,2014,42(21):141.
[11]洪素娣,蒋锁琴,许晓辉,等.肥料运筹对水稻纹枯病和稻曲病的影响[J].江苏农业科学,2013,41(07):127.
 Hong Sudi,et al.Effects of fertilizer management on rice sheath blight and false smut[J].Jiangsu Agricultural Sciences,2013,41(21):127.
[12]刘郁,于亚辉,桑海旭,等.滨海稻区水稻纹枯病对稻米食味及产量的影响[J].江苏农业科学,2015,43(04):122.
 Liu Yu,et al.Effects of sheath blight on taste value and yield of rice in coastal rice region[J].Jiangsu Agricultural Sciences,2015,43(21):122.
[13]王妍,魏松红,王小皙,等.水稻主栽品种对纹枯病的抗性鉴定及评价[J].江苏农业科学,2020,48(13):125.
 Wang Yan,et al.Identification and evaluation of resistance to Rhizoctonia solani of main rice cultivars[J].Jiangsu Agricultural Sciences,2020,48(21):125.
[14]董红刚,耿跃,陈凤,等.水稻纹枯病药剂防治关口前移的用药时间和种类试验[J].江苏农业科学,2021,49(23):125.
 Dong Honggang,et al.Preliminary study on key time forward technology of rice sheath blight control[J].Jiangsu Agricultural Sciences,2021,49(21):125.

备注/Memo

备注/Memo:
收稿日期:2021-12-08
基金项目:黑龙江省农业科学院“农业科技创新跨越工程”(编号:HNK2019CX14);黑龙江省农业科学院科技攻关项目(编号:2021YYYF017)。
作者简介:马军韬(1979—),男,吉林东丰人,硕士,副研究员,主要从事水稻病害及抗病育种研究。Tel:(0451)51127853;E-mail:mmmjjjttt@163.com。
更新日期/Last Update: 2022-11-05