|本期目录/Table of Contents|

[1]陈一帆,薛太强,陈思桥,等.群结腐霉细胞壁降解酶的活性检测及基因表达分析[J].江苏农业科学,2023,51(4):46-51.
　Chen Yifan,et al.Cell wall degrading enzyme activity detection and gene expression analysis in Pythium myriotylum[J].Jiangsu Agricultural Sciences,2023,51(4):46-51.
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群结腐霉细胞壁降解酶的活性检测及基因表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第4期
页码:
46-51
栏目:
生物技术
出版日期:
2023-02-20

文章信息/Info

Title:
Cell wall degrading enzyme activity detection and gene expression analysis in Pythium myriotylum
作者:
陈一帆1; 2;  薛太强2;  陈思桥2;  张金凤2;  周冬梅2;  魏利辉1; 2
1.江苏大学环境与安全工程学院，江苏镇江 212013； 2.江苏省农业科学院植物保护研究所，江苏南京 210014
Author(s):
Chen Yifan; et al
关键词:
群结腐霉; 植物细胞壁降解酶; 转录组; 酶活
Keywords:
-
分类号:
S436.32
DOI:
-
文献标志码:
A
摘要:
群结腐霉(Pythium myriotylum)是一种死体营养型病原菌，可侵染生姜引起茎基腐病，造成生姜产量损失，严重危害该产业发展。群结腐霉含有大量植物细胞壁降解酶(plant cell wall-degrading enzymes，PCWDEs)，然而PCWDEs在群结腐霉侵染生姜过程中的功能和特性却鲜有报道。运用生物信息学手段预测群结腐霉细胞壁降解酶的种类和数量，并以生姜粉末和葡萄糖为碳源，比较菌丝生长的情况，利用转录组分析比较PCWDEs基因在不同碳源条件下的表达差异情况，同时对培养液中主要PCWDEs进行酶活检测。转录组数据显示，与葡萄糖培养基相比，生长在生姜粉末培养基上的群结腐霉中12个PCWDEs编码基因显著上调表达，利用CAZymes网站预测PCWDEs的底物主要为淀粉和纤维素；酶活检测发现，其中α-葡萄糖苷酶和β-葡萄糖苷酶活性较高，分别降解淀粉和纤维素。结果表明，群结腐霉在生姜粉末上生长过程中的PCWDEs以淀粉降解酶和纤维素降解酶为主。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2022-04-12
基金项目：国家现代农业产业技术体系建设专项(编号：CARS-24-C-01)。
作者简介：陈一帆(1997—)，男，浙江温州人，硕士研究生，主要从事生姜茎基腐病害的研究。E-mail：13738370562@163.com。
通信作者：魏利辉，博士，研究员，博士生导师，主要从事蔬菜病害绿色防控研究。E-mail：weilihui@jaas.ac.cn。
更新日期/Last Update: 2023-02-20