|本期目录/Table of Contents|

[1]曾广娟,冯阳,吴舒,等.有机种植与常规种植蔬菜地土壤细菌群落多样性分析[J].江苏农业科学,2023,51(7):197-205.
 Zeng Guangjuan,et al.Analysis of bacterial community diversity under organic and conventional planting[J].Jiangsu Agricultural Sciences,2023,51(7):197-205.
点击复制

有机种植与常规种植蔬菜地土壤细菌群落多样性分析(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第7期
页码:
197-205
栏目:
资源与环境
出版日期:
2023-04-05

文章信息/Info

Title:
Analysis of bacterial community diversity under organic and conventional planting
作者:
曾广娟1冯阳2吴舒1彭红丽1耿世刚1
1.河北环境工程学院,河北秦皇岛 066102; 2.企美实业集团有限公司,河北邯郸 057150
Author(s):
Zeng Guangjuanet al
关键词:
有机种植常规种植高通量测序细菌群落多样性
Keywords:
-
分类号:
S154.3
DOI:
-
文献标志码:
A
摘要:
为揭示有机种植和常规种植2种不同方式对不同土层(0~15 cm和15~30 cm)细菌群落的多样性特征,采用Illumina Miseq高通量测序技术比较了土壤细菌群落结构的差异,利用FAPROTAX软件对获得相应土层细菌群落的功能进行预测。共检测到8 792个OTU,分属于88门152纲319目446科841属371种。有机种植方式的优势菌门为放线菌门、绿弯菌门,而常规种植以变形菌门、酸杆菌门为优势菌门。属水平上,有机种植提高了Haliangium、PelagibiusCastellaniella等有益菌的丰度;常规种植增加了促进氮磷循环和降解农药等有机污染物的细菌丰度,如Candidatus_Entotheonella、Candidatus_Nitrosotenuis、Flavisolibacter、Terrimonas、Sphingomonas、Pseudoxanthomonas,同时常规种植也使得土传病害致病菌Ralstonia的丰度增加;不同土层之间,优势菌属也表现出明显不同。α-多样性指数分析表明,有机种植0~15 cm土层细菌群落丰富度、物种的均匀度均为最高,且与其他土层在observed_species指数、Shannon指数、Simpson指数、Chao1指数、ACE指数有显著差别(P<0.05)。dbRDA分析显示,环境因子对土壤细菌群落的影响大小依次为总氮、土壤有机质、总磷、pH值和总钾。Mantel test检验表明,总氮、土壤有机质、总磷、pH值是影响不同种植方式土壤细菌群落的关键环境因子。上述结果表明,不同种植方式不同土层细菌多样性存在差异,各具优势菌群。有机种植细菌群落的多样性、丰富度和稳定度更高,更有利于增加土壤中的有益菌群。
Abstract:
-

参考文献/References:

[1]寇智瑞,周鑫斌. 不同连作年限黄壤烟田土壤细菌群落的差异[J]. 植物营养与肥料学报,2020,26(3):511-521.
[2]de Ponti T,Rijk B,van Ittersum M K. The crop yield gap between organic and conventional agriculture[J]. Agricultural Systems,2012,108:1-9.
[3]Verena S,Navin R,Foley J A.Comparing the yields of organic and conventional agriculture[J]. Nature,2012,485(7397):229-32.
[4]Fliebach A,Oberholzer H R,Gunst L,et al. Soil organic matter and biological soil quality indicators after 21 years of organic and conventional farming[J]. Agriculture,Ecosystems & Environment,2007,118(1/2/3/4):273-284.
[5]田伟,吴云成,刘明庆,等. 松华坝流域有机蔬菜种植对土壤质量的影响评价[J]. 生态与农村环境学报,2020,36(12):1549-1555.
[6]姜瑢,申思雨,吕贻忠. 华北地区有机种植与常规种植土壤质量比较研究[J]. 土壤,2015,47(4):805-811.
[7]纪荣婷,董刚强,闵炬,等. 有机种植与常规种植体系的比较:基于土壤与肥料的视角[J]. 土壤,2016,48(4):627-633.
[8]李杰,祝凌,仝利红,等. 有机种植对温室土壤肥力质量的影响[J]. 土壤,2019,51(4):690-697.
[9]仝利红,蒋珊,祝凌,等. 有机种植对温室土壤有机碳库和酶活性的影响[J]. 中国土壤与肥料,2020(6):75-82.
[10]王玲,龙飞宇,刘佳斌,等. 有机种植对玉米根际土壤代谢物的影响[J]. 东北农业大学学报,2022,53(3):30-41.
[11]帅艳菊,刘天奇,曹凑贵,等. 有机无机肥配施对玉米-豇豆种植系统土壤N2O排放的影响[J]. 中国生态农业学报,2018,26(12):1763-1772.
[12]林伟,丁军军,李玉中,等. 有机肥和无机肥对菜地土壤N2O排放及其来源的影响[J]. 应用生态学报,2018,29(5):1470-1478.
[13]汪润池,宗良纲,邱晓蕾,等. 有机与常规种植蔬菜地土壤微生物群落特征的比较[J]. 南京农业大学学报,2012,35(2):99-104.
[14]冯海玮,王大欣,毛亮,等. 有机种植对麦田土壤微生物群落影响[J]. 上海交通大学学报(农业科学版),2016,34(5):68-75.
[15]黄化刚,肖谋良,梁士楚,等. 喀斯特山区不同种植方式下烟田土壤微生物特征分析[J]. 烟草科技,2015,48(11):16-21.
[16]朱永官,彭静静,韦中,等. 土壤微生物组与土壤健康[J]. 中国科学(生命科学),2021,51(1):1-11.
[17]Chen Q L,Cui H L,Su J Q,et al. Antibiotic resistomes in plant microbiomes[J]. Trends in Plant Science,2019,24(6):530-541.
[18]Maron P A,Ranjard L,Mougel C,et al. Metaproteomics:a new approach for studying functional microbial ecology[J]. Microbial Ecology,2007,53(3):486-493.
[19]Lundberg D S,Yourstone S,Mieczkowski P,et al. Practical innovations for high-throughput amplicon sequencing[J]. Nature Methods,2013,10(10):999-1002.
[20]王宏燕,孙岩,于军,等. 有机种植对盐碱土主要理化性质的影响[J]. 浙江农业学报,2017,29(9):1544-1548.
[21]Palmer M W,Cooper J,Tétard-Jones C,et al. The influence of organic and conventional fertilisation and crop protection practices,preceding crop,harvest year and weather conditions on yield and quality of potato (Solanum tuberosum) in a long-term management trial[J]. European Journal of Agronomy,2013,49:83-92.
[22]Russo V M,Taylor M. Soil amendments in transition to organic vegetable production with comparison to conventional methods:yields and economics[J]. HortScience,2006,41(7):1576-1583.
[23]Caporaso J G,Kuczynski J,Stombaugh J,et al. QIIME allows analysis of high-throughput community sequencing data[J]. Nature Methods,2010,7(5):335-336.
[24]陈芝兰,张涪平,蔡晓布,等. 秸秆还田对西藏中部退化农田土壤微生物的影响[J]. 土壤学报,2005,42(4):696-699.
[25]陈海生,刘守平,梁国钱,等. 3种西兰花种植方式对根际土壤细菌群落结构和多样性的影响研究[J]. 核农学报,2021,35(6):1457-1465.
[26]李思萌,于军,周正立,等. 有机种植对土壤主要理化性质及重金属含量的影响[J]. 江苏农业科学,2017,45(2):253-257.
[27]Wang Y Z,Xu X M,Liu T M,et al. Analysis of bacterial and fungal communities in continuous-cropping ramie (Boehmeria nivea L.Gaud) fields in different areas in China[J]. Scientific Reports,2020,10:3264.
[28]Johnston-Monje D,Lundberg D S,Lazarovits G,et al. Bacterial populations in juvenile maize rhizospheres originate from both seed and soil[J]. Plant and Soil,2016,405(1):337-355.
[29]何玮,郭琳微,樊鹏辉,等. 黄花棘豆在腐解过程中的化感作用及其土壤细菌群落结构分析[J]. 草业学报,2015,24(7):21-29.
[30]Shen W S,Ni Y Y,Gao N,et al. Bacterial community composition is shaped by soil secondary salinization and acidification brought on by high nitrogen fertilization rates[J]. Applied Soil Ecology,2016,108:76-83.
[31]杜思瑶,于淼,刘芳华,等. 设施种植模式对土壤细菌多样性及群落结构的影响[J]. 中国生态农业学报,2017,25(11):1615-1625.
[32]Navarrete I A,Tsutsuki K. Land-use impact on soil carbon,nitrogen,neutral sugar composition and related chemical properties in a degraded Ultisol in Leyte,Philippines[J]. Soil Science and Plant Nutrition,2010,54(3):321-331.
[33]王鹏,陈波,张华. 基于高通量测序的鄱阳湖典型湿地土壤细菌群落特征分析[J]. 生态学报,2017,37(5):1650-1658.
[34]赵祥,刘红玲,杨盼,等. 滴灌对苜蓿根际土壤细菌多样性和群落结构的影响[J]. 微生物学通报,2019,46(10):2579-2590.
[35]Kundim B A,Itou Y,Sakagami Y,et al. New haliangicin isomers,potent antifungal metabolites produced by a marine myxobacterium[J]. The Journal of Antibiotics,2003,56(7):630-638.
[36]马晓霞,马晓彤,曹卫东,等. 紫云英根瘤菌的系统发育多样性[J]. 应用与环境生物学报,2010,16(3):380-384.
[37]Dean S L,Farrer E C,Taylor D L,et al. Nitrogen deposition alters plant-fungal relationships:linking belowground dynamics to aboveground vegetation change[J]. Molecular Ecology,2014,23(6):1364-1378.
[38]Bell C W,Asao S,Calderon F,et al. Plant nitrogen uptake drives rhizosphere bacterial community assembly during plant growth[J]. Soil Biology and Biochemistry,2015,85:170-182.
[39]胡杰,何晓红,李大平,等. 鞘氨醇单胞菌研究进展[J]. 应用与环境生物学报,2007,13(3):431-437.

相似文献/References:

[1]李思萌,于军,周正立,等.有机种植对土壤主要理化性质及重金属含量的影响[J].江苏农业科学,2017,45(02):253.
 Li Simeng,et al.Effects of organic cultivation on soil physical and chemical properties and heavy metal contents[J].Jiangsu Agricultural Sciences,2017,45(7):253.
[2]王超,章利华,张停林,等.从病程角度阐释有机种植作物病害防控策略[J].江苏农业科学,2019,47(04):86.
 Wang Chao,et al.Plant disease management strategies in organic agriculture from view of plant pathogenesis[J].Jiangsu Agricultural Sciences,2019,47(7):86.
[3]郭柯凡,王丰,娄文月,等.有机种植对滨海稻田氨挥发特征及水稻产量的影响[J].江苏农业科学,2023,51(10):243.
 Guo Kefan,et al.Impacts of organic cultivation on ammonia volatilization and rice yield in coastal paddy field[J].Jiangsu Agricultural Sciences,2023,51(7):243.

备注/Memo

备注/Memo:
收稿日期:2022-05-30
基金项目:河北省科技支撑项目(编号:22374208D);河北省农业生态安全重点实验室开放基金(编号:2020SYSJJ05);河北省秦皇岛市科技局项目(编号:202101A129)。
作者简介:曾广娟(1970—),女,河北昌黎人,博士,教授,研究方向为土壤环境修复。E-mail:guangjuan.z@163.com。
通信作者:耿世刚,教授,研究方向为区域可持续发展,产业生态学。E-mail:gsg1668@163.com。
更新日期/Last Update: 2023-04-05