|本期目录/Table of Contents|

[1]徐小勇,顾铭洲,梁梦鸽,等.枳漆酶基因家族鉴定及其响应盐胁迫的表达分析[J].江苏农业科学,2023,51(9):52-59.
　Xu Xiaoyong,et al.Genome-wide identification of the LAC gene family and its expression analysis under salt stress in Poncirus trifoliata[J].Jiangsu Agricultural Sciences,2023,51(9):52-59.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第9期
页码:
52-59
栏目:
生物技术
出版日期:
2023-05-05

文章信息/Info

Title:
Genome-wide identification of the LAC gene family and its expression analysis under salt stress in Poncirus trifoliata
作者:
徐小勇; 顾铭洲; 梁梦鸽; 姜丽娟
扬州大学园艺园林学院，江苏扬州 225009
Author(s):
Xu Xiaoyong; et al
关键词:
枳; 漆酶基因; 进化树; 顺式作用元件; 盐胁迫
Keywords:
-
分类号:
S188+.3
DOI:
-
文献标志码:
A
摘要:
漆酶(LAC)是植物木质素生物合成中催化木质素单体聚合的关键酶，在调控植物生长发育和胁迫响应中发挥着重要作用。对枳漆酶基因家族进行鉴定和分析，并探究其在盐胁迫下的表达模式，可为进一步研究枳漆酶基因功能提供重要的参考信息。采用生物信息学手段鉴定枳漆酶基因家族成员，对其家族成员的理化性质、基因结构、系统进化关系、启动子顺式作用元件等进行分析，并通过qRT-PCR方法分析其盐胁迫表达模式。结果表明，枳基因组中共有20个LAC基因家族成员，其中18个分布在5条已知染色体上，2个分布在未知染色体上，被预测定位到细胞膜和细胞核中；共有6~14个外显子，5~13个内含子，9~11个motif；系统进化树分析结果显示，20个枳LAC基因家族成员与17个拟南芥LAC基因家族成员共分成7个亚组，20个枳LAC基因家族成员分布在其中的6组；20个枳LAC基因家族成员与拟南芥LAC基因间存在19对共线性关系；启动子区域含有24种顺式作用元件，其中厌氧诱导元件、干旱响应元件和茉莉酸甲酯响应元件数量最多；16个枳LAC基因在盐胁迫下显著上调表达，推测枳漆酶基因参与了盐胁迫响应。
Abstract:
-

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相似文献/References:

[1]郑苗苗,邵淑丽,张令昻,等.红平菇HP1漆酶基因及启动子克隆和序列分析[J].江苏农业科学,2014,42(08):21.
　Zheng Miaomiao,et al.Cloning and sequence analysis of laccase gene and promoter from Pleurotus djamor[J].Jiangsu Agricultural Sciences,2014,42(9):21.

备注/Memo

备注/Memo:
收稿日期：2022-09-26
基金项目：国家重点研发计划(编号：2018YFD1000107)；江苏省农业科技自主创新资金［编号：CX(21)3024］。
作者简介：徐小勇(1979—)，男，江西奉新人，博士，副教授，主要从事果树生物技术研究。E-mail：xyxu@yzu.edu.cn。
更新日期/Last Update: 2023-05-05