|本期目录/Table of Contents|

[1]杨柳,任春梅,陆芳,等.江苏葫芦科作物上瓜类褪绿黄化病毒的发生及其分离物全基因组序列分析[J].江苏农业科学,2024,52(6):36-42.
 Yang Liu,et al.Occurrence of cucurbit chlorinated yellowing virus in Cucurbitaceae crops in Jiangsu Province and whole genome sequence analysis of its isolates[J].Jiangsu Agricultural Sciences,2024,52(6):36-42.
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江苏葫芦科作物上瓜类褪绿黄化病毒的发生及其分离物全基因组序列分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第6期
页码:
36-42
栏目:
生物技术
出版日期:
2024-03-20

文章信息/Info

Title:
Occurrence of cucurbit chlorinated yellowing virus in Cucurbitaceae crops in Jiangsu Province and whole genome sequence analysis of its isolates
作者:
杨柳任春梅陆芳缪倩程兆榜季英华
江苏省农业科学院植物保护研究所,江苏南京 210014
Author(s):
Yang Liuet al
关键词:
瓜类褪绿黄化病毒分布分子鉴定基因组系统进化分析
Keywords:
-
分类号:
S436.429
DOI:
-
文献标志码:
A
摘要:
为了分析瓜类褪绿黄化病毒(cucurbit chlorotic yellow virus,CCYV)在江苏省葫芦科作物上的分布情况、发生趋势以及变异情况,本研究对疑似感染CCYV的葫芦科样本进行采集,覆盖了江苏省8个地区的7种常见葫芦科作物,通过RT-PCR方法对病样进行了分子鉴定,并对在泰州采集的黄瓜分离物CCYV-JSXH进行分段克隆与测序,拼接获得CCYV的全基因组序列后对其进行了序列比对和进化分析。检测结果显示,在建湖采集的西葫芦样本、泰州兴化和南京江宁地区采集的黄瓜样本均有CCYV感染。序列拼接与比对结果显示,分离物CCYV-JSXH的RNA1序列与目前已经公布的各个分离物基因组序列相似度在99.58%以上,在进化树上与山东西葫芦分离物聚为一支;其RNA2序列与各分离株的相似性为99.47%以上,在进化上与广东分离物聚为一支。本研究结果表明,CCYV可能由多个途径传入江苏,并已经扩大蔓延到江苏省多个地区和多种寄主上,对该病毒的监测防控已逐渐扩大为葫芦科作物生产中亟待解决的突出问题之一。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2023-04-28
基金项目:国家重点研发计划(编号:2022YFD1401200);江苏省农业科技自主创新资金[编号:CX(19)3108]。
作者简介:杨柳(1984—),女,江苏徐州人,博士,助理研究员,主要从事蔬菜作物病毒病研究。E-mail:suiyangy@126.com。
通信作者:季英华,研究员,主要从事蔬菜病毒病研究。E-mail:jiyinghua@jaas.ac.cn。
更新日期/Last Update: 2024-03-20