|本期目录/Table of Contents|

[1]徐凯祥,焦玚,王帅帅,等.陆地棉GhRTE基因家族的鉴定与耐盐性分析[J].江苏农业科学,2024,52(6):52-60.
 Xu Kaixiang,et al.Identification and salt tolerance analysis of GhRTE gene family in Gossypium hirsutum[J].Jiangsu Agricultural Sciences,2024,52(6):52-60.
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陆地棉GhRTE基因家族的鉴定与耐盐性分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第6期
页码:
52-60
栏目:
生物技术
出版日期:
2024-03-20

文章信息/Info

Title:
Identification and salt tolerance analysis of GhRTE gene family in Gossypium hirsutum
作者:
徐凯祥焦玚王帅帅陈全家郑凯
新疆农业大学农学院/棉花教育部工程研究中心,新疆乌鲁木齐830052
Author(s):
Xu Kaixianget al
关键词:
陆地棉RTE基因家族非生物胁迫表达特性分析耐盐性
Keywords:
-
分类号:
S562.01
DOI:
-
文献标志码:
A
摘要:
RTE(reversion-to-ethylene)是一种植物乙烯响应的负调节因子,参与植物生长发育并在非生物胁迫的逆境中起着重要作用。通过对陆地棉(Gossypium hirsutum)RTE家族成员的鉴定及其在盐胁迫下的表达模式进行解析,为进一步探索棉花RTE家族成员的功能提供一定的研究指导。通过棉花全基因组数据,鉴定棉花RTE家族成员,以陆地棉为主要研究对象,结合海岛棉(Gossypium barbadense)、亚洲棉(Gossypium arboreum)、雷蒙德氏棉(Gossypium raimondii)等植物的RTE基因家族成员进行物种内的进化分析。同时,利用转录组学方法,研究陆地棉RTE家族基因的表达量及其对盐胁迫的应答规律。结果表明,棉花RTE蛋白家族共鉴定出24个RTE基因,其中来自陆地棉和海岛棉的各有8个,有4个来自雷蒙德氏棉,4个来自亚洲棉。系统发育树分析将24个RTE基因分成3个亚族,GhRTE基因同一亚族成员间具有相似的基因结构和保守基序。GhRTE启动子区包含了许多与植物激素反应、生长发育和逆境应答密切相关的顺式调控因子。通过对转录组数据分析以及荧光定量分析,初步验证该家族GhRTE6基因可能在棉花生长发育以及盐胁迫响应中发挥作用,可为阐明GhRTE基因耐盐的分子机制提供理论依据。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2023-05-29
基金项目:新疆维吾尔自治区重大科技专项(编号:2022A03004-2)。
作者简介:徐凯祥(1995—),男,甘肃兰州人,硕士研究生,研究方向为棉花耐盐性。E-mail:965762115@qq.com。
通信作者:陈全家,博士,教授,博士生导师,从事棉花遗传育种研究,E-mail:chenqjia@126.com;郑凯,博士,副教授,从事棉花种质资源优异基因挖掘和分子育种研究,E-mail:zhengkai555@126.com。
更新日期/Last Update: 2024-03-20