[1]曾妍,朱雨彤,张紫怡,等.桃PpGATA全基因家族生物信息学和高温下的表达分析[J].江苏农业科学,2026,54(10):60-70.
　Zeng Yan,et al.Bioinformatics analysis of entire PpGATA gene family and their expression analysis under high temperature[J].Jiangsu Agricultural Sciences,2026,54(10):60-70.
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桃PpGATA全基因家族生物信息学和高温下的表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第10期
页码:
60-70
栏目:
耐温度胁迫基因鉴定
出版日期:
2026-05-20

文章信息/Info

Title:
Bioinformatics analysis of entire PpGATA gene family and their expression analysis under high temperature
作者:
曾妍; 朱雨彤; 张紫怡; 李志坤; 褚文轩; 吴瑞刚
河北工程大学园林与生态工程学院，河北邯郸 056038
Author(s):
Zeng Yan; et al
关键词:
桃; GATA; 全基因组鉴定; 基因家族; 表达分析; 高温胁迫
Keywords:
-
分类号:
S188；S662.101
DOI:
-
文献标志码:
A
摘要:
GATA转录因子是诸多生物过程的重要调控因子，在调控植物光响应、叶绿素合成以及响应非生物环境胁迫等多个生物过程中发挥重要作用。为探究桃PpGATA全基因组特征、深入了解其结构特点与潜在功能，综合利用Ensembl Plants数据库及HMMER工具对桃PpGATA基因家族成员进行系统生物信息学鉴定。通过整合多种生物信息学分析平台，包括MEGA、TBtools和MEME等软件，对该基因家族的染色体分布特征解析、系统发育树构建、蛋白质理化参数测定、基因结构特征分析、保守motif基序识别、蛋白质保守结构域鉴定、基因组共线性关系探讨、启动子区顺式调控元件预测、表达模式分析以及基因本体(GO)功能注释等进行了系统研究。此外，采用实时荧光定量 PCR(qRT-PCR)技术对筛选的候选PpGATA基因进行表达模式分析。结果表明，桃PpGATA基因家族有20个成员，分布于基因组7条染色体上，编码氨基酸数目在131~542 aa之间，蛋白质分子质量为14.3~60.2 ku。根据拟南芥与桃的系统发育关系和结构特征，将PpGATA分为4个亚家族：Ⅰ、Ⅱ、Ⅲ、Ⅳ。保守motif和基因结构分析发现，同一亚家族内的成员表现出相似的结构特征。共线性分析表明，PpGATA成员在6条染色体之间检测到5对片段重复序列。PpGATA基因启动子区域的顺式调控元件包含与非生物胁迫响应相关的调控元件、参与植物激素信号转导的响应元件和调控植物生长发育过程的功能元件。基因表达分析结果显示，与对照相比，PpGATA1、PpGATA20在高温组中基因显著或极显著下调表达，PpGATA9、PpGATA17基因表达水平极显著上调。本研究开展了对20个PpGATA基因结构、进化特征和基因潜在功能等研究，鉴定了桃GATA基因家族成员并进行生物信息学分析，揭示了PpGATA基因家族成员的特征。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2025-07-10
基金项目：邯郸市科技计划(编号：21422012321)；河北省自然科学基金面上项目(编号：C2022402006)。
作者简介：曾妍(2000—)，女，山东泰安人，硕士研究生，主要从事果树逆境生理分子生物学研究。E-mail：2227928950@qq.com。
通信作者：吴瑞刚，博士，讲师，主要从事果树逆境生理与分子生物学研究。E-mail：wuruigang1986@126.com。
更新日期/Last Update: 2026-05-20