[1]奈婕菲,程玉祥.一个杨树GDSL基因组织表达的特性及其在拟南芥异源的表达[J].江苏农业科学,2014,42(03):16-18.
　Nai Jiefei,et al.Tissue expression of a poplar GDSL gene and its heterologous expression analysis in Arabidopsis thaliana[J].Jiangsu Agricultural Sciences,2014,42(03):16-18.
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一个杨树GDSL基因组织表达的特性及其在拟南芥异源的表达()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年03期
页码:
16-18
栏目:
生物技术
出版日期:
2014-03-25

文章信息/Info

Title:
Tissue expression of a poplar GDSL gene and its heterologous expression analysis in Arabidopsis thaliana
作者:
奈婕菲;  程玉祥
东北林业大学/林木遗传育种国家重点实验室，黑龙江哈尔滨 150040
Author(s):
Nai Jiefei; et al
关键词:
毛果杨; 拟南芥; GDSL酯酶; 载体构建; 转基因
Keywords:
-
分类号:
S792.110.4
DOI:
-
文献标志码:
A
摘要:
GDSL酯酶为脂肪水解酶家族的一个分支，参与植物生长发育和防御反应等多种功能。半定量RT-PCR分析结果显示，毛果杨GDSL酯酶基因Potri.002G253400在顶端茎组织中高丰度特异性表达；构建Potri.002G253400-GFP融合的植物表达载体，转化模式植物拟南芥并获得其过量表达的转基因株系7个；激光共聚焦显微镜检测结果显示，GFP荧光蛋白高丰度表达于转基因植株根的细胞壁区域，说明Potri.002G253400蛋白可能定位于细胞壁。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2013-11-02
基金项目：国家高技术研究发展计划(编号：2013AA102702)。
作者简介：奈婕菲(1986—)，女，黑龙江齐齐哈尔人，硕士研究生，主要从事林木遗传育种研究。E-mail：naijiefei@163.com。
通信作者：程玉祥(1972—)，男，教授，主要从事林木分子遗传学研究。E-mail：chengyuxiang@nefu.edu.cn。
更新日期/Last Update: 2014-03-25