[1]郝东利,杨顺瑛,黄亚楠,等.拟南芥铵转运蛋白AtAMT1.3的电生理功能[J].江苏农业科学,2017,45(08):36-40.
　Hao Dongli,et al.Electrophysiological study on Arabidopsis ammonium transporter AtAMT1.3[J].Jiangsu Agricultural Sciences,2017,45(08):36-40.
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拟南芥铵转运蛋白AtAMT1.3的电生理功能()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年08期
页码:
36-40
栏目:
生物技术
出版日期:
2017-04-22

文章信息/Info

Title:
Electrophysiological study on Arabidopsis ammonium transporter AtAMT1.3
作者:
郝东利1; 2;  杨顺瑛1;  黄亚楠1; 2;  苏彦华1
1.中国科学院南京土壤研究所/土壤与农业可持续发展国家重点实验室，江苏南京210008；2.中国科学院大学，北京100049
Author(s):
Hao Dongli; et al
关键词:
拟南芥; 铵转运蛋白AtAMT1.3; 介导; 电生理; 功能特征; 铵吸收系统; 运输机制
Keywords:
-
分类号:
S184；Q946.1
DOI:
-
文献标志码:
A
摘要:
在生理条件下，植物铵的吸收主要由定位于细胞膜上的铵转运蛋白(ammonium transporter，AMT)介导完成，研究模式植物拟南芥AMT的功能特性及调控机制对于解析植物的铵吸收过程具有重要意义。本研究克隆了拟南芥AtAMT1.3并将其在蛙卵异源系统中表达，电生理结果表明，AtAMT1.3 是一个典型的对铵有高度选择性、高亲和的铵吸收系统［Km =(25.5 ± 3.2) μmmol/L］。同时，AtAMT1.3也能介导铵的同系物甲基铵的吸收［Km =(3.3±0.2) mmmol/L］，对铵和甲基铵的吸收具有浓度依赖性和电压依赖性。 AtAMT1.3的铵转运能力不受pH值调控，它的转运底物是NH4+，运输机制是NH4+单向运输。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2016-01-12
基金项目：中国科学院战略性先导科技专项(编号：XDB15030202)。
作者简介：郝东利(1987—)，男，河南周口人，博士，从事分子植物营养学研究。Tel：(025)86881553；E-mail：dlhao@issas.ac.cn。
通信作者：苏彦华，博士，教授，研究员，从事植物营养与分子生物学研究。E-mail：yhsu@ issas.ac.cn。
更新日期/Last Update: 2017-04-20