|本期目录/Table of Contents|

[1]张权,罗毅康,张柏海,等.热环境下黄羽肉鸡心脏MCEE基因表达分析[J].江苏农业科学,2019,47(13):49-51.
　Zhang Quan,et al.On MCEE gene expression in heart of yellow-feather chicken under heat stress[J].Jiangsu Agricultural Sciences,2019,47(13):49-51.
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热环境下黄羽肉鸡心脏MCEE基因表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第13期
页码:
49-51
栏目:
生物技术
出版日期:
2019-07-31

文章信息/Info

Title:
On MCEE gene expression in heart of yellow-feather chicken under heat stress
作者:
张权;  罗毅康;  张柏海;  产燕子;  黄琳琳;  汪寅;  梁嘉铭
广东海洋大学农学院，广东湛江 524088
Author(s):
Zhang Quan; et al
关键词:
黄羽肉鸡; 热应激; 心脏相对质量; MCEE基因表达
Keywords:
-
分类号:
S831.1
DOI:
-
文献标志码:
A
摘要:
为研究热应激对黄羽肉鸡生长性能的影响，对新广黄公鸡和怀乡公鸡进行热应激处理，分析其对黄羽肉鸡体质量、心脏相对质量和心脏MCEE基因表达的影响。试验随机选取新广黄公鸡和怀乡公鸡各60羽，每个品种随机分6组，每组10羽。每个品种的前3组于22~28日龄时，每天进行热处理［(38±1 )℃，8 h/d，7 d，试验组］；每个品种的后3组于22~28日龄时处于室温［(25±1 )℃，对照组］。分别测定新广黄公鸡和怀乡公鸡21、28日龄体质量。于28日龄时随机抽取每组公鸡各2羽，每个品种试验组和对照组各6羽，测定心脏绝对质量并采集心脏左心室组织用于提取总RNA。MCEE基因相对表达量采用2-ΔΔCT法，利用单因素方差分析其显著性；双因素方差分析黄羽肉鸡体质量和心脏相对质量显著性。结果表明，新广黄公鸡和怀乡公鸡21、28日龄体质量以及28日龄心脏绝对质量显著高于怀乡公鸡(P<0.05)，心脏相对质量差异不显著(P>0.05)。热环境处理7 d后，28日龄新广黄公鸡体质量试验组显著低于对照组(P<0.05)，而28日龄怀乡公鸡试验组与对照组差异不显著(P>0.05)；心脏相对质量差异不显著(P>0.05)。新广黄公鸡心脏MCEE基因相对表达量显著高于怀乡公鸡(P<0.05)。热环境显著降低新广黄公鸡心脏MCEE基因相对表达量(P<0.05)，而对怀乡公鸡心脏MCEE基因相对表达量影响不显著(P>0.05)。初步研究说明，热环境显著降低新广黄公鸡体质量及心脏MCEE基因表达水平，而对怀兴公鸡无显著影响(P>0.05)。MCEE基因表达与黄羽肉鸡生长速度相关，在黄羽肉鸡其他组织器官的表达有待进一步研究。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2019-04-11
基金项目：广东海洋大学博士启动项目(编号：101402/R1703)；大学生创新创业项目(编号：CXXL2018148、CXXL2019180)。
作者简介：张权(1977—)，男，安徽阜阳人，博士，讲师，主要从事家禽遗传育种研究。Tel：(0759)2383250；E-mail：zhang_quan@cau.edu.cn。
更新日期/Last Update: 2019-07-05