|本期目录/Table of Contents|

[1]吴海宁,黄志鹏,唐秀梅,等.氮肥减施对花生根际土壤固氮微生物多样性的影响[J].江苏农业科学,2019,47(16):93-97.
 Wu Haining,et al.Effect of nitrogen reduction on diversity of soil nitrogen-fixing microbes in peanut rhizosphere[J].Jiangsu Agricultural Sciences,2019,47(16):93-97.
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氮肥减施对花生根际土壤固氮微生物多样性的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第16期
页码:
93-97
栏目:
遗传育种与耕作栽培
出版日期:
2019-09-16

文章信息/Info

Title:
Effect of nitrogen reduction on diversity of soil nitrogen-fixing microbes in peanut rhizosphere
作者:
吴海宁 黄志鹏 唐秀梅 熊发前 钟瑞春 贺梁琼 韩柱强 蒋菁 刘菁 唐荣华
广西农业科学院经济作物研究所,广西南宁 530007
Author(s):
Wu Haininget al
关键词:
氮肥减施花生根际土固氮微生物多样性
Keywords:
-
分类号:
S154.3
DOI:
-
文献标志码:
A
摘要:
设置施氮N3(64 kg/hm2)、减量施氮N2(51 kg/hm2)、减量施氮N1(34 kg/hm2),不施氮N0共4个施氮水平,对花生单作、玉米花生间作体系中花生根际土壤样品运用Illumina MiSeq高通量测序技术测序并分析固氮微生物的多样性。结果表明,在单作花生根际土中固氮微生物的多样性和丰度随着氮肥的减施表现出升高的趋势,而在间作花生根际土中固氮微生物的多样性和丰度则随着氮肥的减施出现降低的趋势。对24个花生根际土壤样品进行测序分析得到,固氮微生物可操作分类单元(OTUs)在分类学门、科和属水平上可归类为8个门、29个科和37个属,其中在门水平上有3个优势类群,共占所有门的97.7%以上,分别为变形菌门(Proteobacteria)、细菌未分类门(p_unclassified_k_,norank_d_Bacteria)、蓝藻门(Cyanobacteria)。氮肥减施条件下固氮微生物群落结构在单作花生根际土壤中比较相似,在间作花生根际土壤差异较大。试验表明,氮肥减施有利于单作花生根际土壤固氮微生物的生长,增强固氮微生物相对土壤中其他微生物的竞争优势,提高固氮微生物的多样性和丰度。而氮肥减施不利于间作花生根际土壤固氮微生物的生长繁殖,降低了间作花生根际土壤固氮微生物的多样性和丰度。
Abstract:
-

参考文献/References:

[1]Zou Y K,Zhang J N,Yang D L,et al. Effects of different land use patterns on nifH genetic diversity of soil nitrogen-fixing microbial communities in Leymus Chinensis steppe[J]. Acta Ecologica Sinica,2011,31(3):150-156.
[2]冯晓敏,杨永,任长忠,等. 燕麦/大豆和燕麦/花生间作对根际土壤固氮细菌多样性与群落结构的影响[J]. 中国农业大学学报,2016,21(1):22-32.
[3]彭东海,杨建波,李健,等. 间作大豆对甘蔗根际土壤细菌及固氮菌多样性的影响[J]. 植物生态学报,2014,38(9):959-969.
[4]杨亚东,冯晓敏,胡跃高,等. 豆科作物间作燕麦对土壤固氮微生物丰度和群落结构的影响[J]. 应用生态学报,2017,28(3):957-965.
[5]Zhu Y Y,Chen H R,Fan J H,et al.Genetic diversity and disease control in rice[J]. Nature,2000,406:718-722.
[6]Young J P W,Stacy G,Burris R H,et al.Biological nitrogen fixation phylogenic classification of nitrogen-fixing organisms[M]. New York:Chapman and Hall,1992:43-86.
[7]Raymond J,Siefert J L,Staples C R,et al.The natural history of nitrogen fixation[J]. Molecular Biology and Evolution,2004,21(3):541-554.
[8]Zehr J P,Jenkins B D,Short S M,et al . Nitrogenase gene diversity and microbial community structure:a cross-system comparison[J]. Environmental Microbiology,2003,5(7):539-554.
[9]Dixon R,Kahn D.Genetic regulation of biological nitrogen fixation[J]. Nature Reviews Microbiology,2004(2):621-631.
[10]Orr C H,James A,Leifert C,et al.Diversity and activity of free-living nitrogen-fixing bacteria and total bacteria in organic and conventionally managed soils[J]. Applied and Environmental Microbiology,2011,77(3):911-919.
[11]Mirza B S,Potisap C,Nüsslein K,et al.Response of free-living nitrogen-fixing microorganisms to land use change in the Amazon rainforest[J]. Applied and Environmental Microbiology,2014,80(1):281-288.
[12]Wang J C,Zhang D,Zhang L,et al.Temporal variation of diazotrophic community abundance and structure in surface and subsoil under four fertilization regimes during a wheat growing season[J]. Agriculture Ecosystems & Environment,2016,216:116-124.
[13]郑棉海,陈浩,朱晓敏,等. 矿质养分输入对森林生物固氮的影响[J]. 生态学报,2015,35(24):7941-7954.
[14]周晶. 长期施氮对东北黑土微生物及主要氮循环菌群的影响[D]. 北京:中国农业大学,2017:85-108.

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备注/Memo

备注/Memo:
收稿日期:2018-05-07
基金项目:国家自然科学基金(编号:31660371);广西科技开发项目(编号:桂科转1599004-12);现代农业产业技术体系建设专项资金(编号:CARS-13-华南区栽培);广西自然科学基金(编号:2017GXNSFAA198144);广西农业科学院科技发展基金(编号:桂农科2017JM18)。
作者简介:吴海宁(1986—),男,广西扶绥人,硕士,助理研究员,主要从事花生栽培与育种研究工作。E-mail:wuhaining86@qq.com。
通信作者:唐荣华,博士,研究员,主要从事花生高产高效栽培与育种研究工作。E-mail:tronghua@163.com。
更新日期/Last Update: 2019-08-20