|本期目录/Table of Contents|

[1]陈海元,朱晓妹,张所兵,等.2个水稻叶片卷曲变窄突变体的遗传分析和基因定位[J].江苏农业科学,2021,49(2):37-43.
　Chen Haiyuan,et al.Genetic analysis and gene mapping of two rolling and narrow leaf mutants in rice[J].Jiangsu Agricultural Sciences,2021,49(2):37-43.
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2个水稻叶片卷曲变窄突变体的遗传分析和基因定位(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第2期
页码:
37-43
栏目:
遗传育种与耕作栽培
出版日期:
2021-01-20

文章信息/Info

Title:
Genetic analysis and gene mapping of two rolling and narrow leaf mutants in rice
作者:
陈海元;  朱晓妹;  张所兵;  张云辉;  方先文
江苏省农业科学院种质资源与生物技术研究所，江苏南京 210014
Author(s):
Chen Haiyuan; et al
关键词:
水稻; 叶片卷曲变窄; 突变体; 基因定位; NRL2
Keywords:
-
分类号:
S511.01
DOI:
-
文献标志码:
A
摘要:
通过甲基磺酸乙酯(EMS)诱变水稻品种南粳46，发现2个可以稳定遗传的叶片卷曲变窄的突变体rnl1-1(rolling and narrow leaf1-1)和rnl1-2。与野生型相比，突变体从苗期开始表现出叶片卷曲变窄的表型，一直持续到成熟期。此外，突变体抽穗期延迟，株高、穗长、剑叶长、剑叶宽、一次枝梗数、二次枝梗数、粒厚、每穗粒数和粒宽均显著降低。遗传分析表明，突变体的突变表型受1对隐性基因控制。利用rnl1-1与籼稻品种Dular杂交建立F2群体，将该基因定位在水稻第12染色体长臂上的InDel标记HF36和HF43之间约277 kb 的区间内。该区间内NRL2基因突变引起的表型与rnl突变体相似，测序发现rnl1-1突变体中RNL2基因的第1708位核苷酸由C突变为T，使得编码的氨基酸由丝氨酸突变成脯氨酸；rnl1-2突变体中RNL2基因的第2011位核苷酸由G突变为A，使得编码的氨基酸由精氨酸突变成甘氨酸。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2020-04-10
基金项目：江苏省自然科学基金(编号：BK20180309)。
作者简介：陈海元(1988—)，男，江苏徐州人，博士，助理研究员，主要从事水稻基因功能解析及分子设计育种研究。Tel：(025)84390321；E-mail：15150530179@163.com。
通信作者：方先文，博士，研究员，主要从事水稻品种资源研究。Tel：(025)84390321；E-mail：2431240491@qq.com。
更新日期/Last Update: 2021-01-20