[1]张轶敏,王东,王煜,等.棘孢木霉PT-29代谢产物抑制镰刀菌毒素的产生[J].江苏农业科学,2023,51(21):126-132.
　Zhang Yimin,et al.Trichoderma asperellum PT-29 metabolites inhibit fusarium toxin production[J].Jiangsu Agricultural Sciences,2023,51(21):126-132.
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棘孢木霉PT-29代谢产物抑制镰刀菌毒素的产生()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第21期
页码:
126-132
栏目:
植物保护
出版日期:
2023-11-05

文章信息/Info

Title:
Trichoderma asperellum PT-29 metabolites inhibit fusarium toxin production
作者:
张轶敏; 王东; 王煜; 郝建秀; 周洪友
内蒙古农业大学园艺与植物保护学院/生物农药创制与资源利用自治区高等学校重点实验室(培育)，内蒙古呼和浩特 010018
Author(s):
Zhang Yimin; et al
关键词:
棘孢木霉; 生物防治; 尖孢镰刀菌; 镰刀菌酸; 次级代谢物
Keywords:
-
分类号:
S435.32
DOI:
-
文献标志码:
A
摘要:
为了解棘孢木霉(Trichoderma asperellum)PT-29的抑菌机制及其代谢产物对尖孢镰刀菌(Fusarium oxysporum)ZY-3-1的生防作用，采用双培养试验、对扣试验、培养滤液试验，测定棘孢木霉PT-29及其代谢产物对尖孢镰刀菌ZY-3-1的拮抗作用，并使用荧光定量PCR和高效液相色谱技术，定量分析了棘孢木霉代谢产物对尖孢镰刀菌ZY-3-1中镰刀菌酸的抑制作用。结果表明，棘孢木霉PT-29对尖孢镰刀菌ZY-3-1的抑菌率达6364%，同时增强了马铃薯植株对尖孢镰刀菌的抗性；木霉代谢液、挥发性物质对尖孢镰刀菌ZY-3-1的抑菌率分别达52.22%、42.86%。木霉代谢产物处理抑制镰刀菌酸相关合成基因的表达；木霉代谢液处理后，尖孢镰刀菌中镰刀菌素含量为0.003 3 μg/mL，木霉挥发性代谢产物处理后，尖孢镰刀菌中镰刀菌素的含量为0031 8 μg/mL，均显著低于对照组菌株镰刀菌素的含量(0.086 0 μg/mL)。本研究初步解释了棘孢木霉对尖孢镰刀菌的生防机制，棘孢木霉PT-29的代谢产物在防治马铃薯枯萎病中展现出巨大潜力，可为马铃薯枯萎病的防治提供新的思路和见解。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2023-02-07
基金项目：内蒙古自治区重点研发项目(编号：2022YFHH0036)；中央引导地方科技发展资金(编号：2021ZY0005)；内蒙古自治区科技重大专项(编号：2021ZD0005)。
作者简介：张轶敏(1996—)，女，内蒙古鄂尔多斯人，硕士研究生，主要研究方向为有害生物综合防治。E-mail：1305070717@qq.com。
通信作者：周洪友，博士，教授，研究方向为植物病害防治及植物病理学。E-mail：hongyouzhou2002@a
更新日期/Last Update: 2023-11-05