[1]刘宏祥,朱春红,宋卫涛,等.鸭胚胎发育后期胸肌萎缩相关通路及基因鉴定[J].江苏农业科学,2023,51(22):190-199.
　Liu Hongxiang,et al.Identification of pathways and genes associated with pectoral muscle atrophy in late embryonic development of ducks[J].Jiangsu Agricultural Sciences,2023,51(22):190-199.
点击复制

鸭胚胎发育后期胸肌萎缩相关通路及基因鉴定()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第22期
页码:
190-199
栏目:
畜牧兽医与水产蚕桑
出版日期:
2023-12-04

文章信息/Info

Title:
Identification of pathways and genes associated with pectoral muscle atrophy in late embryonic development of ducks
作者:
刘宏祥; 朱春红; 宋卫涛; 陶志云; 徐文娟; 章双杰; 王志成; 李慧芳
江苏省家禽科学研究所，江苏扬州 225125
Author(s):
Liu Hongxiang; et al
关键词:
鸭; 胚胎; 胸肌; WGCNA; GSEA
Keywords:
-
分类号:
S834.2
DOI:
-
文献标志码:
A
摘要:
与哺乳动物不同，禽类胚胎在卵中发育，在胚胎后期表现为胸肌萎缩，为探明其涉及的分子机制，探索高邮鸭和金定鸭在孵化后18胚龄(ed)至出雏后7日龄(d)的胸肌发育规律，在21 ed、27 ed和5 d 3个时间点采集胸肌样品进行转录组测序(RNA-seq)。采用加权基因共表达网络分析(WGCNA)，以及基于KEGG数据库的过表征(ORA-KEGG)和基因集富集分析(GSEA-KEGG)2种功能富集分析方法，鉴定与27 ed胸肌萎缩特征相关的信号通路及相关基因；最后通过实时荧光定量PCR(qRT-PCR)方法验证WGCNA得到的棕色模块中核心基因的表达水平。高邮鸭和金定鸭的胸肌质量均从18 ed到24 ed迅速增加，24 ed开始到1 d逐渐下降，1 d后继续增加。通过WGCNA得到的7个共表达模块中棕色模块与27 ed的相关性最高(r=0.98，P=2×10-12)，该模块内的基因通过ORA-KEGG方法富集到了氧化磷酸化和柠檬酸循环2条能量代谢相关的信号通路。通过GSE-KEGG方法对所有转录表达的基因功能富集，挖掘到了自噬相关的信号通路。qRT-PCR验证的棕色模块内基因中，自噬通路中的突触体相关蛋白29(SNAP29)和蛋白激酶AMP激活的催化亚基α2(PRKAA2)基因在27 ed时表达量显著增加(P<0.05)。在胚胎后期，鸭胸肌出现发育停滞甚至萎缩，这可能是胸肌动员降解以释放能量从而应对能量紧张状态的结果。在这一过程中，能量传感器AMPK的激活和自噬途径中SNAP29等关键基因表达的升高可能是胸肌蛋白质降解途径激活的原因。
Abstract:
-

参考文献/References:

［1］Smith J H. Relation of body size to muscle cell size and number in the chicken［J］. Poultry Science，1963，42(2)：283-290.
［2］Rehfeldt C，Stickland N C，Fiedler I，et al. Environmental and genetic factors as sources of variation in skeletal muscle fibre number［J］. Basic and Applied Myology，1999，9(5)：235-254.
［3］Picard B，Lefaucheur L，Berri C，et al. Muscle fibre ontogenesis in farm animal species［J］. Reproduction Nutrition Development，2002，42(5)：415-431.
［4］Swatland H J. Muscle growth in the fetal and neonatal pig［J］. Journal of Animal Science，1973，37(2)：536-545.
［5］Chen W，Tangara M，Xu J，et al. Developmental transition of pectoralis muscle from atrophy in late-term duck embryos to hypertrophy in neonates［J］. Experimental Physiology，2012，97(7)：861-872.
［6］Gu L H，Xu T S，Huang W，et al. Developmental characteristics of pectoralis muscle in Pekin duck embryos［J］. Genetics and Molecular Research，2013，12(4)：6733-6742.
［7］Moore D T. The influence of early nutrition on muscle development in the poult［D］. North Carolina：North Carolina State University，2005.
［8］Moore D T，Ferket P R，Mozdziak P E. Muscle development in the late embryonic and early post-hatch poult［J］. International Journal of Poultry Science，2005，4(3)：138-142.
［9］Chen W，Lv Y T，Zhang H X，et al. Developmental specificity in skeletal muscle of late-term avian embryos and its potential manipulation［J］. Poultry Science，2013，92(10)：2754-2764.
［10］李德生. 胚胎期外源性营养物质介入对鹅胸肌及肠道发育规律的影响研究［D］. 长春：吉林农业大学，2016.
［11］Trapnell C，Pachter L，Salzberg S L. TopHat：discovering splice junctions with RNA-Seq［J］. Bioinformatics，2009，25(9)：1105-1111.
［12］Anders S，Pyl P T，Huber W. HTSeq-A Python framework to work with high-throughput sequencing data［J］. Bioinformatics，2015，31(2)：166-169.
［13］Mortazavi A，Williams B A，Mccue K，et al. Mapping and quantifying mammalian transcriptomes by RNA-Seq［J］. Nature Methods，2008，5(7)：621-628.
［14］Langfelder P，Horvath S. WGCNA：an R package for weighted correlation network analysis［J］. BMC Bioinformatics，2008，9(1)：559.
［15］Yip A M，Horvath S. Gene network interconnectedness and the generalized topological overlap measure［J］. BMC Bioinformatics，2007，8：22.
［16］Li G H，Zhang T，Zhang G X，et al. Analysis of gene co-expression networks and function modules at different developmental stages of chicken breast muscle［J］. Biochemical and Biophysical Research Communications，2019，508(1)：177-183.
［17］Wu T Z，Hu E Q，Xu S B，et al. clusterProfiler 4.0：a universal enrichment tool for interpreting omics data［J］. The Innovation，2021，2(3)：100141.
［18］Yang C Y，Zhu Y，Ding Y L，et al. Identifying the key genes and functional enrichment pathways associated with feed efficiency in cattle［J］. Gene，2022，807：145934.
［19］Wan Q，Tang J，Han Y，et al. Co-expression modules construction by WGCNA and identify potential prognostic markers of uveal melanoma［J］. Experimental Eye Research，2018，166：13-20.
［20］Wang R，Xue Y S，Fan J，et al. A systems genetics approach reveals PbrNSC as a regulator of lignin and cellulose biosynthesis in stone cells of pear fruit［J］. Genome Biology，2021，22(1)：313.
［21］Yang Y，Han L，Yuan Y，et al. Gene co-expression network analysis reveals common system-level properties of prognostic genes across cancer types［J］. Nature Communications，2014，5(1)：3231.
［22］Jin X J，Li J，Li W，et al. Weighted gene co-expression network analysis reveals specific modules and biomarkers in Parkinsons disease［J］. Neuroscience Letters，2020，728：134950.
［23］Lehninger A L，Wadkins C L，Cooper C，et al. Oxidative phosphorylation［J］. Science，1958，128(3322)：450-456.
［24］Korzeniewski B. The modeling of oxidative phosphorylation in skeletal muscle［J］. The Japanese Journal of Physiology，2004，54(6)：511-516.
［25］Foye O T. The biochemical and molecular effects of amniotic nutrient administration，“in OVO feeding” on intestinal development and function and carbohydrate metabolism in turkey embryos and poult［D］. North Carolina：North Carolina State University，2005.
［26］Chen W，Wang R，Wan H F，et al. Influence of in ovo injection of glutamine and carbohydrates on digestive organs and pectoralis muscle mass in the duck［J］. British Poultry Science，2009，50(4)：436-442.
［27］Milan G，Romanello V，Pescatore F，et al. Regulation of autophagy and the ubiquitin-proteasome system by the FoxO transcriptional network during muscle atrophy［J］. Nature Communications，2015，6：6670.
［28］Mammucari C，Milan G，Romanello V，et al. FoxO3 controls autophagy in skeletal muscle in vivo［J］. Cell Metabolism，2007，6(6)：458-471.
［29］Matsui T，Jiang P，Nakano S，et al. Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17［J］. The Journal of Cell Biology，2018，217(8)：2633-2645.
［30］Tian X Y，Teng J L，Chen J G. New insights regarding SNARE proteins in autophagosome-lysosome fusion［J］. Autophagy，2021，17(10)：2680-2688.
［31］Takats S，Nagy P，Varga ，et al. Autophagosomal Syntaxin17-dependent lysosomal degradation maintains neuronal function in Drosophila［J］. The Journal of Cell Biology，2013，201(4)：531-539.
［32］Guo B，Liang Q Q，Li L，et al. O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation［J］. Nature Cell Biology，2014，16(12)：1215-1226.
［33］Xiao B，Sanders M J，Underwood E，et al. Structure of mammalian AMPK and its regulation by ADP［J］. Nature，2011，472(7342)：230-233.
［34］Hardie D G，Sakamoto K. AMPK：a key sensor of fuel and energy status in skeletal muscle［J］. Physiology，2006，21(1)：48-60.
［35］Herzig S，Shaw R J. AMPK：guardian of metabolism and mitochondrial homeostasis［J］. Nature Reviews Molecular Cell Biology，2018，19(2)：121-135.
［36］Thomson D M. The role of AMPK in the regulation of skeletal muscle size，hypertrophy，and regeneration［J］. International Journal of Molecular Sciences，2018，19(10)：3125.
［37］Martin-Rincon M，Morales-Alamo D，Calbet J A L. Exercise-mediated modulation of autophagy in skeletal muscle［J］. Scandinavian Journal of Medicine & Science in Sports，2018，28(3)：772-781.
［38］Hardie D G. AMPK-sensing energy while talking to other signaling pathways［J］. Cell Metabolism，2014，20(6)：939-952.
［39］Kim J，Kundu M，Viollet B，et al. AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1［J］. Nature Cell Biology，2011，13(2)：132-141.
［40］Weerasekara V K，Panek D J，Broadbent D G，et al. Metabolic-stress-induced rearrangement of the 14-3-3ζ interactome promotes autophagy via a ULK1-and AMPK-regulated 14-3-3ζ interaction with phosphorylated Atg9［J］. Molecular and Cellular Biology，2014，34(24)：4379-4388.
［41］Zhang D Y，Wang W，Sun X J，et al. AMPK regulates autophagy by phosphorylating BECN1 at threonine 388［J］. Autophagy，2016，12(9)：1447-1459.

相似文献/References:

[1]刘玉华,陈光明,方向红,等.泰州地区鸭源大肠杆菌的分离鉴定与耐药性试验[J].江苏农业科学,2014,42(12):260.
　Liu Yuhua,et al.Identification and drug resistance test of duck Escherichia coli in Taizhou area[J].Jiangsu Agricultural Sciences,2014,42(22):260.
[2]陈学文,陈再忠.中华青鳉胚胎低温保存研究[J].江苏农业科学,2013,41(10):192.
　Chen Xueweng,et al.Study on cryopreservation of Chinese medaka embryo[J].Jiangsu Agricultural Sciences,2013,41(22):192.
[3]张敬峰,李银,赵冬敏,等.鸭源坦布苏病毒株NJX-4的分离鉴定及部分生物学特性[J].江苏农业科学,2013,41(11):228.
　Zhang Jingfeng,et al.Isolation，identification and partial biological characteristics of duck-derived Tembusu virus strain NJX-4[J].Jiangsu Agricultural Sciences,2013,41(22):228.
[4]王淑娟,刘文举,王立克,等.褪黑激素对雌性动物生殖系统调节作用的研究进展[J].江苏农业科学,2016,44(06):15.
　Wang Shujuan,et al.Research progress of regulating effect of mellocated atonin on female reproductive system[J].Jiangsu Agricultural Sciences,2016,44(22):15.
[5]宋迟,宋卫涛,胡艳,等.鸭肝脏IGF1基因早期发育表达[J].江苏农业科学,2015,43(08):42.
　Song Chi,et al.Gene expression profile of IGF1 mRNA in duck liver during early development[J].Jiangsu Agricultural Sciences,2015,43(22):42.
[6]李迎晓,安辉,焦凤超,等.鸭大肠杆菌与沙门氏菌混合感染的分离鉴定及药敏试验[J].江苏农业科学,2015,43(04):224.
　Li Yingxiao,et al.Isolation and drug sensitivity of co-infection of Escherichia coli and Salmonella[J].Jiangsu Agricultural Sciences,2015,43(22):224.
[7]詹 年,李运生,韩春杨,等.安徽白山羊超数排卵技术[J].江苏农业科学,2015,43(02):214.
　Zhan Nian,et al.Study on superovulation of Anhui white goat[J].Jiangsu Agricultural Sciences,2015,43(22):214.
[8]章双杰,王靖,杨建生,等.长三角地区兼用型鸭全基因组遗传多样性分析[J].江苏农业科学,2017,45(17):32.
　Zhang Shuangjie,et al.Genetic diversity analysis of whole genome of duck in Yangtze Delta region[J].Jiangsu Agricultural Sciences,2017,45(22):32.
[9]王永娟,董亚青,郭方超,等.鸭源H9N2亚型禽流感病毒RT-PCR检测方法的建立[J].江苏农业科学,2018,46(1):105.
　Wang Yongjuan,et al.Establishment of RT-PCR assay for detecting H9N2 subtype avian influenza virus in ducks[J].Jiangsu Agricultural Sciences,2018,46(22):105.
[10]彭新亮,伦峰,郭旭升,等.黄尾鲴胚胎及仔鱼的发育[J].江苏农业科学,2018,46(22):164.
　Peng Xinliang,et al.Embryonic and larval development of Xenocypris davidi[J].Jiangsu Agricultural Sciences,2018,46(22):164.

备注/Memo

备注/Memo:
收稿日期：2023-05-08
基金项目：江苏省种业振兴“揭榜挂帅”项目(编号：JBGS［2021］111、JBGS［2021］030)；苏州市科技计划(编号：SNG2020068)；江苏省现代农业(水禽)产业技术体系项目(编号：JATS［2022］404)。
作者简介：刘宏祥(1985—)，男，江苏仪征人，硕士，副研究员，主要从事家禽遗传资源保护与利用研究。E-mail：lhxatyz@foxmail.com。
通信作者：李慧芳，博士，研究员，主要从事家禽遗传资源保护与利用研究。E-mail：lhfxf_002@aliyun.com。
更新日期/Last Update: 2023-11-20