[1]刘淑娟,刘腾云,刘立盘,等.UV-B不同剂量辐照下生菜生长和代谢组学分析[J].江苏农业科学,2024,52(8):131-138.
 Liu Shujuan,et al.Growth and metabolomics analysis of lettuce under different doses of UV-B irradiation[J].Jiangsu Agricultural Sciences,2024,52(8):131-138.
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UV-B不同剂量辐照下生菜生长和代谢组学分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第8期
页码:
131-138
栏目:
园艺与林学
出版日期:
2024-04-20

文章信息/Info

Title:
Growth and metabolomics analysis of lettuce under different doses of UV-B irradiation
作者:
刘淑娟1刘腾云1刘立盘1朱安繁2黄星瑜2欧阳雪灵3周华1
1.江西省科学院生物资源研究所,江西南昌 330096; 2.江西省农业技术推广中心,江西南昌 330046; 3.江西省经济作物研究所,江西南昌 330202
Author(s):
Liu Shujuanet al
关键词:
UV-B辐照生菜剂量生长代谢产物
Keywords:
-
分类号:
S636.204
DOI:
-
文献标志码:
A
摘要:
以奶油生菜为研究材料,在植物工厂人工光源中设置3种紫外线B(UV-B)剂量[0、8.64、17.28 kJ/(m2·d)]处理,利用广靶代谢组学技术,分析不同剂量UV-B辐照下生菜叶片中的差异代谢物质,研究UV-B辐照对生菜生物量、光合参数和次生代谢物的影响。结果表明,与0剂量相比,低剂量UV-B处理对生菜生物量、光合参数均未产生显著影响,代谢组检测到30种差异代谢产物,主要为生物碱、酚类、萜类等物质,其中9种上调表达,21种下调表达;高剂量UV-B处理显著降低了生菜地上部干重、地下部干重、气孔导度和蒸腾速率,但地上部鲜重、净光合速率均未呈现显著差异,共检测到35个差异表达代谢物,其中21种上调表达,14种下调表达,上调表达的代谢物主要为黄酮类、单萜等,生菜叶片槲皮素含量在低剂量、高剂量UV-B处理下均显著增加。研究结果可为利用UV-B辐照提高设施生菜营养品质提供理论依据和参考。
Abstract:
-

参考文献/References:

[1]Caldwell M M,Bornman J F,Ballaré C L,et al. Terrestrial ecosystems,increased solar ultraviolet radiation,and interactions with other climate change factors[J]. Photochem Photobiol Sci,2007,6(3):252-266.
[2]Hideg E,Jansen M A K,Strid . UV-B exposure,ROS,and stress:inseparable companions or loosely linked associates?[J]. Trends Plant Sci,2013,18(2):107-115.
[3]王修蘋,李想,李祖然,等. 植物对UV-B辐射增强响应的跨代可塑性特征及机制研究进展[J]. 植物生理学报,2022,58(5):797-805.
[4]黄志慧,张一宁,李娜娜,等. 增补UV-B辐射对菥蓂生理特性及次生代谢产物的影响[J]. 植物研究,2022,42(6):1079-1087.
[5]Rodríguez-Calzada T,Qian M,Strid ,et al. Effect of UV-B radiation on morphology,phenolic compound production,gene expression,and subsequent drought stress responses in chili pepper (Capsicum annuum L.)[J]. Plant Physiology and Biochemistry,2019,134:94-102.
[6]Takshak S,Agrawal S B. Secondary metabolites and phenylpropanoid pathway enzymes as influenced under supplemental ultraviolet-B radiation in Withania somnifera Dunal,an indigenous medicinal plant[J]. J Photoch Photobio B,2014,140:332-343.
[7]Quintero-Arias D G,Acua-Caita J F,Asensio C,et al. Ultraviolet transparency of plastic films determines the quality of lettuce (Lactuca sativa L.) grown in a greenhouse[J]. Agronomy,2021,11(2):358.
[8]Dai Q J,Yan B,Huang S B,et al. Response of oxidative stress defense systems in rice (Oryza sativa) leaves with supplemental UV-B radiation[J]. Physiol Plantarum,1997,101(2):301-308.
[9]Loconsole D,Santamaria P. UV lighting in horticulture:a sustainable tool for improving production quality and food safety[J]. Horticulturae,2021,7(1):9.
[10]Jenkins G I. Signal transduction in responses to UV-B radiation[J]. Annu Rev Plant Biol,2009,60(1):407.
[11]Tsurunaga Y,Takahashi T,Katsube T,et al. Effects of UV-B irradiation on the levels of anthocyanin,rutin and radical scavenging activity of buckwheat sprouts[J]. Food Chemistry,2013,141(1):552-556.
[12]孙令强,李召虎,段留生,等. UV-B辐射对黄瓜幼苗生长和光合作用的影响[J]. 华北农学报,2006,21(6):79-82.
[13]岳堃,王红,郭钰柬,等. 增强UV-B辐射对芒果叶片光合组织结构的损伤[J]. 热带生物学报,2019,10(4):324-330.
[14]Wang H B,Gui M Y,Tian X,et al. Effects of UV-B on vitamin C,phenolics,flavonoids and their related enzyme activities in mung bean sprouts (Vigna radiata)[J]. Int J Food Sci Tech,2017,52(3):827-833.
[15]Lee J H. UV-B signal transduction pathway in Arabidopsis[J]. J Integr Plant Biol,2016,59(3):223-230.
[16]Bergstrand K J,Schüssler H K. Recent progresses on the application of LEDs in the horticultural production[J]. Acta Hortic,2012,927:529-534.
[17]Massot C,Génard M,Stevens R,et al. Fluctuations in sugar content are not determinant in explaining variations in vitamin C in tomato fruit[J]. Plant Physiol Bioch,2010,48(9):751-757.
[18]赵晓莉,胡正华,徐建强,等. UV-B辐射与酸雨胁迫对生菜生理特性及品质的影响[J]. 生态环境,2006,15(6):1170-1175.
[19]陈乾,刘洋,肖丽君,等. 超高效液相色谱串联质谱法同时测定叶菜中13种抗生素[J]. 环境科学,2020,41(2):952-961.
[20]刘榕晨. UV-B辐射对‘大金星’山楂生理特性及次生代谢产物的影响[D]. 太谷:山西农业大学,2019.
[21]陈慧泽,韩榕. 植物响应UV-B辐射的研究进展[J]. 植物学报,2015,50(6):790-801.
[22]Huché-Thélier L,Crespel L,Le Gourrierec J,et al. Light signaling and plant responses to blue and UV radiations perspectives for applications in horticulture[J]. Environ Exp Bot,2016,121:22-38.
[23]Yao Y,Xuan Z,He Y,et al. Principal component analysis of intraspecific responses of tartary buckwheat to UV-B radiation under field conditions[J]. Environ Exp Bot,2007,61(3):237-245.
[24]Wargent J J,Elfadly E M,Moore J P,et al. Increased exposure to UV-B radiation during early development leads to enhanced photoprotection and improved long-term performance in Lactuca sativa[J]. Plant Cell Environ,2011,34(8):1401-1413.
[25]Wargent J J,Nelson B C,McGhie T K,et al. Acclimation to UV-B radiation and visible light in Lactuca sativa involves up-regulation of photosynthetic performance and orchestration of metabolome-wide responses[J]. Plant Cell Environ,2015,38(5):929-940.
[26]Topcu Y,Dogan A,Sahin-Nadeem H,et al. Morphological and biochemical responses of broccoli florets to supplemental ultraviolet-B illumination[J]. Agr Ecosyst Environ,2018,259:1-10.
[27]Agati G,Tattini M. Multiple functional roles of flavonoids in photoprotection[J]. New Phytol,2010,186(4):786-793.
[28]Agati G,Azzarello E,Pollastri S,et al. Flavonoids as antioxidants in plants:location and functional significance[J]. Plant Sci,2012,196:67-76.
[29]刘佳钰,王蓉,张弛,等. UV-B辐射与植物次级代谢产物变化的研究进展[J]. 基因组学与应用生物学,2017,36(8):3157-3166.
[30]何淼,王霁佳,高文杰,等. UV-B辐射对神农香菊萜类物质合成及其相关基因表达的影响[J]. 广西植物,2019,39(7):933-939.
[31]Eichholz I,Huyskens-Keil S,Keller A,et al. UV-B-induced changes of volatile metabolites and phenolic compounds in blueberries (Vaccinium corymbosum L.)[J]. Food Chem,2011,126(11):60-64.
[32]Gil M,Bottini R,Pontin M,et al. Solar UV-B radiation modifies the proportion of volatile organic compounds in flowers of field-grown grapevine (Vitis vinifera L.) cv. Malbec[J]. Plant Growth Regul,2014,74(2):193-197.
[33]Schreiner M,Martínez-Abaigar J,Glaab J,et al. UV‐B induced secondary plant metabolites:potential benefits for plant and human health[J]. Optik & Photonik,2014,9(2):34-37.
[34]Takshak S,Agrawal S B. Defense potential of secondary metabolites in medicinal plants under UV-B stress[J]. J Photoch Photobio B,2019,193:51-88.
[35]Meyer P,van de Poel B,de Coninck B. UV-B light and its application potential to reduce disease and pest incidence in crops[J]. Hortic Res,2021,8(1):194.

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备注/Memo

备注/Memo:
收稿日期:2023-04-28
基金项目:国家自然科学基金(编号:31960623);江西省科学院重大产业技术攻关专项(编号:2023YSBG10005)。
作者简介:刘淑娟(1982—),女,安徽宿州人,博士,副研究员,主要从事植物遗传育种研究。E-mail:liushujuan@126.com。
通信作者:周华,博士,研究员,主要从事设施园艺研究。E-mail:yuerhua116@126.com。
更新日期/Last Update: 2024-04-20