[1]佟晓楠,胡文娟,杨杰,等.枳PtrPP2C51基因克隆与非生物胁迫表达分析[J].江苏农业科学,2024,52(10):49-54.
 Tong Xiaonan,et al.Cloning and abiotic stress expression analysis of PtrPP2C51 gene in Poncirus trifoliata[J].Jiangsu Agricultural Sciences,2024,52(10):49-54.
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PtrPP2C51基因克隆与非生物胁迫表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第10期
页码:
49-54
栏目:
生物技术
出版日期:
2024-05-20

文章信息/Info

Title:
Cloning and abiotic stress expression analysis of PtrPP2C51 gene in Poncirus trifoliata
作者:
佟晓楠胡文娟杨杰陈凯董小筠钟奕恬张晓媛李兴涛
赣南师范大学生命科学学院/国家脐橙工程技术研究中心,江西赣州 341000
Author(s):
Tong Xiaonanet al
关键词:
PP2C基因基因克隆非生物胁迫
Keywords:
-
分类号:
S666.01
DOI:
-
文献标志码:
A
摘要:
克隆枳PP2C基因家族的PtrPP2C51基因,分析其在低温、高温和干旱等胁迫下的表达规律,探究其在非生物胁迫中的功能。以1年生枳苗为试材,从嫩叶克隆到PtrPP2C51基因cDNA序列,采用生物信息学方法对其进行分析,利用实时荧光定量PCR仪(qRT-PCR)检测枳苗在低温、高温和干旱(15%PEG-6000)处理下的PtrPP2C51基因表达模式。克隆获得PtrPP2C51基因编码区CDS序列长度为879 bp,编码292个氨基酸,其蛋白分子量为31.36 ku,理论等电点4.9,脂肪系数79.52,不稳定系数39.57,亲水性平均值-0.279。PtrPP2C51蛋白的二级结构中α-螺旋、β-转角、延伸链和无规则卷曲占比分别为40.07%、7.88%、18.15%和33.90%。PtrPP2C51蛋白与克里曼丁橘的PP2C蛋白在同一小分支上,亲缘关系最近。经低温(0 ℃)、高温(38 ℃)和干旱(15%PEG-6000)3种不同胁迫处理,PtrPP2C51基因的相对表达量均表现持续上调趋势,并且均在处理12 h时表达量达到最高值。PtrPP2C51基因可能参与枳的抗逆胁迫调控机制。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2023-07-19
基金项目:国家自然科学基金(编号:32160731);江西省教育厅科技项目(编号:GJJ2201233)。
作者简介:佟晓楠(1983—),女,辽宁辽阳人,硕士,讲师,主要从事柑橘低温胁迫分子机制研究。E-mail:tongxn_gnnu@qq.com。
通信作者:李兴涛,博士,副教授,研究方向为果树逆境植物生理。E-mail:lixt.gnnu@qq.com。
更新日期/Last Update: 2024-05-20