|本期目录/Table of Contents|

[1]魏浩卓,李宁,李静泉.稻瘟病拮抗菌短小芽孢杆菌NDY-10的功能特性与全基因组分析[J].江苏农业科学,2024,52(12):277-284.
　Wei Haozhuo,et al.Functional characteristics of Bacillus pumilus NDY-10 against Magnaporthe oryzae and its genome-wide analysis[J].Jiangsu Agricultural Sciences,2024,52(12):277-284.
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稻瘟病拮抗菌短小芽孢杆菌NDY-10的功能特性与全基因组分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第12期
页码:
277-284
栏目:
资源与环境
出版日期:
2024-06-20

文章信息/Info

Title:
Functional characteristics of Bacillus pumilus NDY-10 against Magnaporthe oryzae and its genome-wide analysis
作者:
魏浩卓; 李宁; 李静泉
内蒙古大学生态与环境学院，内蒙古呼和浩特 010021
Author(s):
Wei Haozhuo; et al
关键词:
短小芽孢杆菌; 稻瘟病菌; 拮抗作用; 生物防治; 全基因组
Keywords:
-
分类号:
S182；S435.111.4+1
DOI:
-
文献标志码:
A
摘要:
短小芽孢杆菌(Bacillus pumilus)NDY-10对植物病原菌具有广谱拮抗活性，其中对稻瘟病菌的抑菌率最高。从NDY-10菌株对稻瘟病菌的拮抗特性和全基因组分析2个方面进行研究，结果表明，NDY-10菌株对稻瘟病病菌具有非常强的抑制作用，其无菌发酵液对稻瘟病菌的抑制率可达100%，且与添加量呈正相关。NDY-10菌株无菌发酵液对温度、紫外线辐射不敏感，强酸或强碱会影响其拮抗活性。经3种蛋白酶处理后，NDY-10菌株对稻瘟病病菌的抑制率下降，说明短小芽孢杆菌NDY-10菌株对稻瘟病病菌的拮抗物质是一种蛋白。NDY-10菌株的完整基因组大小为3 736 781 bp(含41.63%的G+C)，包括3 893个编码序列(CDS)。从该基因组中鉴定出9条基因簇，其中大部分负责抑制代谢物的合成，并且发现了tasA拮抗基因(801对碱基)。根据之前的研究，推测其为稻瘟病接抗菌NDY-10菌株的关键功能基因。由本研究结果可知，NDY-10菌株可以作为潜在的优秀的生防制剂用于防治稻瘟病。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2023-07-18
基金项目：内蒙古自治区自然科学基金(编号：2018MS03074、2022MS03049)。
作者简介：魏浩卓(1998—)，女，内蒙古通辽人，硕士研究生，研究方向为环境微生物学。E-mail：wee74893@163.com。
通信作者：李静泉，博士，讲师，研究方向为环境微生物学。E-mail：ljq198327@163.com。
更新日期/Last Update: 2024-06-20