[1]范丽珍,佟晓楠,陈凯,等.枳DREB基因家族的鉴定及其对非生物胁迫的响应[J].江苏农业科学,2024,52(15):53-64.
 Fan Lizhen,et al.Genome-wide identification and abiotic stress responses of DREB gene family in Poncirus trifoliata[J].Jiangsu Agricultural Sciences,2024,52(15):53-64.
点击复制

枳DREB基因家族的鉴定及其对非生物胁迫的响应()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第15期
页码:
53-64
栏目:
生物技术
出版日期:
2024-08-05

文章信息/Info

Title:
Genome-wide identification and abiotic stress responses of DREB gene family in Poncirus trifoliata
作者:
范丽珍佟晓楠陈凯钟静郑淑芳张欣悦董小筠王心蕊胡子菲李兴涛
赣南师范大学生命科学学院/国家脐橙工程技术研究中心,江西赣州 341000
Author(s):
Fan Lizhenet al
关键词:
DREB基因家族生物信息学非生物胁迫
Keywords:
-
分类号:
S184;S666.401
DOI:
-
文献标志码:
A
摘要:
为了解枳(Poncirus trifoliata)DREB基因家族参与非生物胁迫的调控机制,基于枳基因组数据,利用生物信息学方法对枳DREB基因家族成员(PtrDREB)进行全基因组鉴定,讨论PtrDREB各基因的物种进化关系及各基因在不同组织中的表达模式,并用 qRT-PCR 技术检测9个PtrDREB家族成员在非生物胁迫处理下的相对表达量。结果表明,从枳基因组中共鉴定出48个DREB成员,不均匀地分布在9条染色体上,这些DREB成员编码的蛋白质氨基酸序列长度范围为144~639 aa,相对分子量范围为15 638.35~76 776.34 u,等电点的范围为4.72~9.62,均为亲水性蛋白;34个PtrDREB亚细胞定位于细胞核中;48个PtrDREB基因分为5个亚族;DREB基因的启动子顺式作用元件含有大量光响应、植物激素、非生物胁迫及植物生长发育响应元件;DREB基因家族在不同组织中的表达具有明显差异,在根、果实、叶片、胚珠、种子和幼芽中高表达的基因数目分别为17、13、4、4、6、8个;qRT-PCR数据表明,在经过低温、高温和干旱处理后,9个PtrDREB基因中分别有8、6、1个基因上调表达,上调幅度分别为1.27~5.85、1.44~2.05、206倍,因此推测PtrDREB家族成员在非生物胁迫中可能发挥重要的调控作用。
Abstract:
-

参考文献/References:

[1]刘坤,李国婧,杨杞. 参与植物非生物逆境响应的DREB/CBF转录因子研究进展[J]. 生物技术通报,2022,38(5):201-214.
[2]Nakano T,Suzuki K,Fujimura T,et al. Genome-wide analysis of the ERF gene family in Arabidopsis and rice[J]. Plant Physiology,2006,140(2):411-432.
[3]Zhuang J,Deng D X,Yao Q H,et al. Discovery,phylogeny and expression patterns of AP2-like genes in maize[J]. Plant Growth Regulation,2010,62(1):51-58.
[4]Zhou Y X,Zhou W,Liu H,et al. Genome-wide analysis of the soybean DREB gene family:identification,genomic organization and expression profiles in response to drought stress[J]. Plant Breeding,2020,139(6):1158-1167.
[5]Thamilarasan S K,Park J I,Jung H J,et al. Genome-wide analysis of the distribution of AP2/ERF transcription factors reveals duplication and CBFs genes elucidate their potential function in Brassica oleracea[J]. BMC Genomics,2014,15:422.
[6]Niu X,Luo T L,Zhao H,et al. Identification of wheat DREB genes and functional characterization of TaDREB3 in response to abiotic stresses[J]. Gene,2020,740:144514.
[7]Feng W Q,Li J,Long S X,et al. A DREB1 gene from zoysiagrass enhances Arabidopsis tolerance to temperature stresses without growth inhibition[J]. Plant Science:an International Journal of Experimental Plant Biology,2019,278:20-31.
[8]Latha G M,Raman K V,Lima J M,et al. Genetic engineering of indica rice with AtDREB1A gene for enhanced abiotic stress tolerance[J]. Plant Cell,Tissue and Organ Culture,2019,136(1):173-188.
[9]Shi H T,Qian Y Q,Tan D X,et al. Melatonin induces the transcripts of CBF/DREB1s and their involvement in both abiotic and biotic stresses in Arabidopsis[J]. Journal of Pineal Research,2015,59(3):334-342.
[10]Du X P,Li W Y,Sheng L P,et al. Over-expression of chrysanthemum CmDREB6 enhanced tolerance of chrysanthemum to heat stress[J]. BMC Plant Biology,2018,18(1):178.
[11]Hong B,Ma C,Yang Y J,et al. Over-expression of AtDREB1A in chrysanthemum enhances tolerance to heat stress[J]. Plant Molecular Biology,2009,70(3):231-240.
[12]王灿. 黄瓜CsHSFA1功能鉴定及DREB家族生物信息学分析[D]. 泰安:山东农业大学,2022:46-55.
[13]冯军,郑彩霞. DREB转录因子在植物非生物胁迫中的作用及应用研究[J]. 植物生理学报,2011,47(5):437-442.
[14]严佳文.柑橘内参基因筛选及转pthA基因甜橙的表达分析[D]. 长沙:湖南农业大学,2010:31.
[15]Livak K J,Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods,2001,25(4):402-408.
[16]Maqsood H,Munir F,Amir R,et al. Genome-wide identification,comprehensive characterization of transcription factors,cis-regulatory elements,protein homology,and protein interaction network of DREB gene family in Solanum lycopersicum[J]. Frontiers in Plant Science,2022,13:1031679.
[17]Mushtaq N,Munir F,Gul A,et al. Genome-wide analysis,identification,evolution and genomic organization of dehydration responsive element-binding (DREB) gene family in Solanum tuberosum[J]. PeerJ,2021,9:e11647.
[18]Ghorbani R,Zakipour Z,Alemzadeh A,et al. Genome-wide analysis of AP2/ERF transcription factors family in Brassica napus[J]. Physiology and Molecular Biology of Plants,2020,26(7):1463-1476.
[19]Ma L T,Zhu T,Wang H R,et al. Genome-wide identification,phylogenetic analysis and expression profiling of the late embryogenesis-abundant (LEA) gene family in Brachypodium distachyon[J]. Functional Plant Biology,2021,48(4):386-401.
[20]Qin F,Kakimoto M,Sakuma Y,et al. Regulation and functional analysis of ZmDREB2A in response to drought and heat stresses in Zea mays L.[J]. The Plant Journal,2007,50(1):54-69.
[21]Chen M,Xu Z S,Xia L Q,et al. Cold-induced modulation and functional analyses of the DRE-binding transcription factor gene,GmDREB3,in soybean (Glycine max L.)[J]. Journal of Experimental Botany,2009,60(1):121-135.
[22]Chen M,Wang Q Y,Cheng X G,et al. GmDREB2,a soybean DRE-binding transcription factor,conferred drought and high-salt tolerance in transgenic plants[J]. Biochemical and Biophysical Research Communications,2007,353(2):299-305.
[23]Oh S J,Song S I,Kim Y S,et al. Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth[J]. Plant Physiology,2005,138(1):341-351.
[24]吴关庭,郎春秀,胡张华,等. 转CBF1基因增强水稻的耐逆性[J]. 核农学报,2006,20(3):169-173.
[25]Liu X Q,Liu C Y,Guo Q,et al. Mulberry transcription factor MnDREB4A confers tolerance to multiple abiotic stresses in transgenic tobacco[J]. PLoS One,2015,10(12):e0145619.
[26]孙瑞芬,张艳芳,聂利珍,等. 向日葵HaDREBA5基因克隆及其对生物和非生物胁迫的响应[J]. 农业生物技术学报,2021,29(5):900-914.
[27]Watanabe K N,Kikuchi A,Shimazaki T,et al. Salt and drought stress tolerances in transgenic potatoes and wild species[J]. Potato Research,2011,54(4):319-324.
[28]Behnam B,Kikuchi A,Celebi-Toprak F,et al. Arabidopsis rd29A::DREB1A enhances freezing tolerance in transgenic potato[J]. Plant Cell Reports,2007,26(8):1275-1282.

相似文献/References:

[1]徐小勇,顾铭洲,梁梦鸽,等.枳漆酶基因家族鉴定及其响应盐胁迫的表达分析[J].江苏农业科学,2023,51(9):52.
 Xu Xiaoyong,et al.Genome-wide identification of the LAC gene family and its expression analysis under salt stress in Poncirus trifoliata[J].Jiangsu Agricultural Sciences,2023,51(15):52.
[2]佟晓楠,胡文娟,杨杰,等.枳PtrPP2C51基因克隆与非生物胁迫表达分析[J].江苏农业科学,2024,52(10):49.
 Tong Xiaonan,et al.Cloning and abiotic stress expression analysis of PtrPP2C51 gene in Poncirus trifoliata[J].Jiangsu Agricultural Sciences,2024,52(15):49.
[3]李欣远,刘鑫,朱倩,等.PtGRAS基因家族鉴定及在干旱胁迫中的响应模式[J].江苏农业科学,2026,54(9):34.
 Li Xinyuan,et al.Identification of PtGRAS gene family and its response model to drought stress[J].Jiangsu Agricultural Sciences,2026,54(15):34.

备注/Memo

备注/Memo:
收稿日期:2023-09-27
基金项目:国家自然科学基金(编号:32160731);江西省教育厅科学技术研究项目(编号:GJJ2201233);赣南师范大学研究生创新基金(编号:YCX23A038)。
作者简介:范丽珍(1995—),女,江西九江人,硕士研究生,主要从事柑橘功能基因挖掘与验证方面的研究。E-mail:1035934736@qq.com。
通信作者:李兴涛,博士,副教授,主要从事果树逆境植物生理方面的研究。E-mail:lixt.gnnu@qq.com。
更新日期/Last Update: 2024-08-05