|本期目录/Table of Contents|

[1]刘陈玮,徐仁超,卞晓春,等.低温和赤霉素对蚕豆开花、内源激素、基因表达的调控[J].江苏农业科学,2024,52(24):105-112.
 Liu Chenwei,et al.Regulating effects of low temperature and gibberellin on flowering,endogenous hormones,and gene expression of broad bean[J].Jiangsu Agricultural Sciences,2024,52(24):105-112.
点击复制

低温和赤霉素对蚕豆开花、内源激素、基因表达的调控(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第24期
页码:
105-112
栏目:
遗传育种与耕作栽培
出版日期:
2024-12-20

文章信息/Info

Title:
Regulating effects of low temperature and gibberellin on flowering,endogenous hormones,and gene expression of broad bean
作者:
刘陈玮徐仁超卞晓春王凡陆红臣王玉娟吴春芳
江苏沿江地区农业科学研究所/南通市作物春化技术重点实验室,江苏南通 226000
Author(s):
Liu Chenweiet al
关键词:
蚕豆低温赤霉素开花内源激素关键基因
Keywords:
-
分类号:
S643.601
DOI:
-
文献标志码:
A
摘要:
蚕豆是长三角地区重要的越冬作物之一,但其产量低、成熟慢和采摘期短,让鲜食蚕豆销售遭遇很大障碍。人工低温春化技术的研发推广,实现蚕豆鲜荚提前上市,采摘期大幅延长。赤霉素是一类非常重要的植物激素,参与植物生长发育等多个生物学过程。为了探究赤霉素和低温联合应用对蚕豆花期及生理特性的影响,并为进一步调控蚕豆花期提供理论支撑和实践依据,以蚕豆品种通鲜1号、通鲜2号、通鲜3号、启豆2号、南通三白为材料,对蚕豆芽苗进行低温处理、赤霉素喷施处理、低温+赤霉素喷施处理,统计始花时间、单株开花数量、株高、分枝数、主茎节数等开花和生长特性,并检测不同发育阶段叶片内源激素和开花相关基因的表达情况。结果表明,低温处理可以使蚕豆开花提早16~22 d,喷施赤霉素处理可以促进开花提早2~10 d,低温+100 mg/L赤霉素处理提早开花效果最佳,最早可提前26 d。低温和100 mg/L 赤霉素均可以促进蚕豆分枝数和主茎节数的增加,低温会抑制蚕豆株高,但赤霉素处理会显著增加株高。以促进开花效果最好的通鲜3号为材料,发现ABA、IAA、GA3含量在蚕豆苗期、蕾期、花期均呈先增后降的趋势,低温处理会降低蚕豆叶片中ABA的含量,提高IAA、GA3的含量,适宜的赤霉素处理可以提高叶片中的ABA、IAA、GA3含量。通过实时荧光定量PCR研究发现,低温、赤霉素、低温+赤霉素处理均可以诱导GA20ox、GID1、FT、SOC1、VRN1的表达,抑制GAI的表达,VIN3只响应低温的调控。蚕豆芽苗低温处理后,叶面喷施100 mg/L赤霉素可以有效提早开花期,并提高分枝数、主茎节数、株高,可在鲜食蚕豆生产上进行应用。
Abstract:
-

参考文献/References:

[1]卞晓春,王凡,吴春芳. 春化蚕豆产业现状及发展建议[J]. 现代农业科技,2021,17 (17):245-247.
[2]徐兵划,祁婷,曹玉杰,等. 蚕豆人工春化诱导技术研究[J]. 湖北农业科学,2015,54(19):4673-4675,4681.
[3]戚自荣,崔萌萌,王旭强,等. 鲜食蚕豆不同人工春化处理及栽培技术试验[J]. 中国园艺文摘,2017,33(7):31-33.
[4]陈江辉,王旭强,王双千,等. 春化处理对蚕豆性状表达的影响[J]. 浙江农业科学,2018,59(1):6-8.
[5]赵薇,王爱花,陆慢,等. 不同春化处理对蚕豆开花结荚时间和产量的影响[J]. 江苏农业科学,2018,46(16):102-105.
[6]Song S W,Lei Y L,Huang X M,et al. Crosstalk of cold and gibberellin effects on bolting and flowering in flowering Chinese cabbage[J]. Journal of Integrative Agriculture,2019,18(5):992-1000.
[7]Sheng J Y,Li X,Zhang D. Gibberellins,brassinolide,and ethylene signaling were involved in flower differentiation and development in Nelumbo nucifera[J]. Horticultural Plant Journal,2022,8(2):243-250.
[8]李茂福,杨媛,王华,等. 赤霉素对露地栽培月季卡罗拉生长发育的影响[J]. 园艺学报,2019,46(4):749-760.
[9]邵奇,袁星星,于龙龙,等. 人工春化对不同基因型蚕豆生长表型及发育形态的影响[J]. 江苏农业科学,2016,44(9):218-221.
[10]中国科学院植物生理研究所生长素工作组. 赤霉酸对蚕豆生长的影响[J]. 实验生物学报,1959,6(3):211-216.
[11]夏明忠. 赤霉素和其他生长调节剂对蚕豆生长的协同效应[J].植物生理学通讯,1990(4):20-23.
[12]Hiroko Y,Nobutaka S,Akihiro I,et al. Involvement of gibberellins in the regulation of tillering in Welsh onion (Allium fistulosum L.)[J]. The Horticulture Journal,2015,84(4):334-341.
[13]Mujadidi A,Kumar M,Malik S,et al. Effect of time and concentrations of gibberellic acid application on growth and flowering of African marigold (Tagetes erecta L.) CV. Pusa Narangi[J]. Progressive Agriculture,2019,19(2):293-297.
[14]Sharma A,Dilta B S,Sharma C L,et al. Effect of foliar application of gibberellic acid on plant growth,flowering and yield attributes in pansy (Viola×wittrockiana Gams.)[J]. International Journal of Plant & Soil Science,2023,35(17):403-410.
[15]黄家总,颜艳,冈田芳明,等. 温度与赤霉素对紫罗兰生育及其开花的影响[J]. 广州大学学报(自然科学版),2004,3(1):27-30.
[16]Wen Y,Su S C,Ma L Y,et al. Effects of gibberellic acid on photosynthesis and endogenous hormones of Camellia oleifera Abel. in 1st and 6th leaves[J]. Journal of Forest Research,2018,23(5):309-317.
[17]Qiu L H,Chen R F,Luo H M,et al. Effects of exogenous GA3 and DPC treatments on levels of endogenous hormone and expression of key gibberellin biosynthesis pathway genes during stem elongation in sugarcane[J]. Sugar Tech,2019,21(6):936-948.
[18]章金辉,王再花,李杰,等. 低温和植物生长调节剂诱导春石斛‘Carmen’开花及其生理变化[J]. 北方园艺,2020(18):64-70.
[19]Zhou L P,Pan C,Wang M X,et al. Progress on the mechanism of hormones regulating plant flower formation[J]. Hereditas,2020,42(8):739-751.
[20]赵文武,黄莉娟,赵丽丽,等. 低温胁迫对葛藤幼苗生长发育及生理特性的影响[J]. 南方农业学报,2022,53(11):3068-3078.
[21]周琴,张思思,包满珠,等. 高等植物成花诱导的分子机理研究进展[J]. 分子植物育种,2018,16(11):3681-3692.
[22]Neha S,Koen G,Shekhar B G,et al. The molecular mechanism of vernalization in Arabidopsis and cereals:role of flowering locus C and its homologs[J]. Physiologia Plantarum,2020,170(3):373-383.
[23]杨艳华,张亚东,朱镇,等. 赤霉素(GA3)和脱落酸(ABA)对不同水稻品种生长和生理特性及GA20ox2、GA3ox2基因表达的影响[J]. 中国水稻科学,2010,24(4):433-437.
[24]Yue J H,Zhang D,Chen G Q,et al. ApGA20ox1,a key gibberellin biosynthesis gene,regulates somatic embryogenesis and plant height in Agapanthus praecox[J]. Scientia Horticulturae,2023,312:111846.
[25]张玉喜,张文超,李玉娥,等. 赤霉素氧化酶PsGA20ox基因参与低温诱导的牡丹内休眠解除[J]. 华北农学报,2014,29(3):22-26.
[26]王春生. 大豆GmGAI基因参与赤霉素调控开花时间的功能研究[D]. 哈尔滨:东北农业大学,2021.
[27]李鹏. GA对扁桃花药发育的影响及调控网络构建[D]. 乌鲁木齐:新疆农业大学,2019.
[28]范树国. 高等植物成花分子机理研究进展[J]. 楚雄师范学院学报,2014,29(6):58-69.
[29]Yong W J,Areum L,Sun J M,et al. Genome-wide identification of flowering time genes associated with vernalization and the regulatory flowering networks in Chinese cabbage[J]. Plant Biotechnology Reports,2018,12(5):347-363.

相似文献/References:

[1]王素平,孙艳军.H2O2预处理对低温下黄瓜幼苗抗氧化酶同工酶的影响[J].江苏农业科学,2013,41(06):123.
 Wang Suping,et al.Effect of H2O2 pretreatment on antioxidant isoenzyme in cucumber seedlings under chilling stress[J].Jiangsu Agricultural Sciences,2013,41(24):123.
[2]雷忻,卢利梅,王文强,等.双酚A胁迫对蚕豆根尖细胞微核的影响[J].江苏农业科学,2013,41(08):375.
 Lei Xin,et al.Effect of bisphenol A stress on micronucleus in root tip cells of Vicia faba[J].Jiangsu Agricultural Sciences,2013,41(24):375.
[3]王素平.钙和水杨酸对低温下黄瓜幼苗抗氧化酶同工酶的影响[J].江苏农业科学,2016,44(03):168.
 Wang Suping.Effects of Ca2+ and SA pretreatment on antioxidase isoforms in cucumber seedlings under chilling stress[J].Jiangsu Agricultural Sciences,2016,44(24):168.
[4]汪凯华,王学军,缪亚梅,等.优质鲜食大粒蚕豆通蚕鲜8号的选育和栽培要点[J].江苏农业科学,2013,41(11):113.
 Wang Kaihua,et al.Breeding and cultivation techniques of fresh-eaten broad bean cultivar “Tongcanxian No.8” with big grain and high quality[J].Jiangsu Agricultural Sciences,2013,41(24):113.
[5]缪亚梅,王学军,汪凯华,等.种植密度对通蚕鲜7号农艺性状、产量及经济效益的影响[J].江苏农业科学,2013,41(11):173.
 Miao Yamei,et al.Effects of planting density on agronomic traits,yield and economic benefits of broad bean cultivar “Tongcanxian No.7”[J].Jiangsu Agricultural Sciences,2013,41(24):173.
[6]缪亚梅,王学军,汪凯华,等.不同基因型蚕豆品质构成因子的比较分析[J].江苏农业科学,2013,41(12):96.
 Miao Yamei,et al.Comparative analysis of quality factors of different genotypes of broad beans[J].Jiangsu Agricultural Sciences,2013,41(24):96.
[7]秀洪学,陈建斌,汤东生,等.氮肥对蚕豆结瘤和植株生长的影响[J].江苏农业科学,2014,42(01):82.
 Xiu Hongxue,et al.Effects of nitrogen fertilizer on nodulation and growth of broad bean[J].Jiangsu Agricultural Sciences,2014,42(24):82.
[8]薛杨,景元书,谭孟祥,等.不同灌溉方式对抽穗扬花期晚稻抵御低温的影响[J].江苏农业科学,2016,44(04):105.
 Xue Yang,et al.Effects of different irrigation methods on withstand low temperatures for late rice at earing and flowering stage[J].Jiangsu Agricultural Sciences,2016,44(24):105.
[9]彭杰,王伟中,顾大路.抗低温冷害调理剂在水稻上的应用效果[J].江苏农业科学,2016,44(02):99.
 Peng Jie,et al.Application effect of anti chilling conditioner in rice[J].Jiangsu Agricultural Sciences,2016,44(24):99.
[10]杨文飞,顾大路,朱云林,等.小麦喷施新型氨基酸肥料(粒宝宝)的效果[J].江苏农业科学,2015,43(09):109.
 Yang Wenfei,et al.Effect of new type of amino acid fertilizer on growth of wheat[J].Jiangsu Agricultural Sciences,2015,43(24):109.

备注/Memo

备注/Memo:
收稿日期:2023-11-24
基金项目:江苏省种业振兴“揭榜挂帅”项目(编号:JBGS〔2021〕058);江苏现代农业产业技术体系建设项目(编号:JATS[2022]169);江苏省重点研发计划(现代农业)(编号:BE2021389);沿江所青年科技基金[编号:YJ(2021)007]。
作者简介:刘陈玮(1993—),男,江苏南通人,硕士,助理研究员,主要从事蚕豆春化及病虫害防控研究。E-mail:20192001@jaas.ac.cn。
通信作者:吴春芳,硕士,研究员,主要从事春化蚕豆育种及栽培技术等研究。E-mail:jsyias@163.com。
更新日期/Last Update: 2024-12-20