[1]高逸飞,李波,郑聪,等.烟草烟碱代谢相关基因表达分析[J].江苏农业科学,2025,53(2):23-34.
　Gao Yifei,et al.Analysis of gene expression related to nicotine metabolism in tobacco[J].Jiangsu Agricultural Sciences,2025,53(2):23-34.
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烟草烟碱代谢相关基因表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第2期
页码:
23-34
栏目:
生物技术
出版日期:
2025-01-20

文章信息/Info

Title:
Analysis of gene expression related to nicotine metabolism in tobacco
作者:
高逸飞1; 李波2; 郑聪3; 陈小翔2; 谢可3; 刘亚冉4; 张智强1
1.河南农业大学烟草学院，河南郑州450002； 2.浙江中烟工业有限责任公司，浙江杭州310008； 3.福建省烟草公司南平市公司浦城分公司，福建浦城 353400； 4.河南烟草投资管理有限公司，河南郑州450002
Author(s):
Gao Yifei; et al
关键词:
烟碱; 合成基因; 转运基因; 调控基因; 荧光定量; 表达模式
Keywords:
-
分类号:
S572.01
DOI:
-
文献标志码:
A
摘要:
为了系统地研究烟草烟碱相关合成、运输、调控基因在不同组织器官的表达情况，以K326苗期7~75 d和盛花期的组织器官样品为材料，采用荧光定量PCR的方法，通过对烟碱合成、运输、调控基因表达量的测定，探究烟碱相关基因的表达模式。表达模式分析结果表明，烟碱合成基因NtAO、NtQS、NtQPT1、NtQPT2、NtADC、NtMPO1、NtMPO2、NtSPDS、NtBBLa，烟碱转运基因NtJAT1、NtJAT2、NtMATE1、NtMATE2、NtGEF，烟碱调控基因NtMYC2a、NtMYC2b、NtERF32、NtERF91、NtARF6、NtLNP1、Nt-miRNA2635为组成型表达，在各组织中均有表达；而烟碱合成基因NtPMTa1、NtA622，烟碱转运基因NtNUP1和烟碱调控基因NtERF189在根中特异高表达，在其他组织部位表达量极低或几乎不表达。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2023-12-29
基金项目：福建省烟草公司南平市公司项目(编号：2022350700240103)；浙江中烟工业有限责任公司项目(编号：2023330000340127、ZJZY2021B009)。
作者简介：高逸飞(1997—)，女，河南郑州人，硕士研究生，主要从事烟草遗传育种与品种改良研究。E-mail：15637184739@163.com。
通信作者：张智强，博士，讲师，主要从事烟草遗传育种与品质改良研究。E-mail：xiao_qiang8866@163.com。
更新日期/Last Update: 2025-01-20