|本期目录/Table of Contents|

[1]汪自松,杨正丽,曾梓芸,等.化肥减量配施有机肥对芒果根际微生物多样性及酶活性的影响[J].江苏农业科学,2025,53(2):240-247.
 Wang Zisong,et al.Effects of fertilizer reduction combined with organic fertilizer on rhizosphere microbial diversity and enzyme activity of Mangifera indica L.[J].Jiangsu Agricultural Sciences,2025,53(2):240-247.
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化肥减量配施有机肥对芒果根际微生物多样性及酶活性的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第2期
页码:
240-247
栏目:
资源与环境
出版日期:
2025-01-20

文章信息/Info

Title:
Effects of fertilizer reduction combined with organic fertilizer on rhizosphere microbial diversity and enzyme activity of Mangifera indica L.
作者:
汪自松12杨正丽2曾梓芸2沈伟12方利娟12刘正鲁12赵志常3
1.百色学院广西芒果生物学重点实验室,广西百色 533000; 2.百色学院农业与食品工程学院,广西百色 533000; 3.中国热带农业科学院热带作物品种资源研究所,海南海口 571101
Author(s):
Wang Zisonget al
关键词:
芒果化肥减施土壤酶活性土壤微生物多样性NovaSeq测序
Keywords:
-
分类号:
S667.706
DOI:
-
文献标志码:
A
摘要:
为探究化肥减量配施有机肥对芒果根际土壤细菌、真菌群落组成和结构的影响,开展田间定位试验,设CK(仅施用化肥)和T1(化肥减量10%+有机肥6.0 t/hm2)、T2(化肥减量20%+有机肥9.0 t/hm2)、T3(有机肥、无机肥等量复混)、T4(有机肥、无机肥减量10%复混)、T5(有机肥、无机肥减量20%复混)5个处理。利用 NovaSeq测序平台,分析土壤细菌、真菌群落结构和组成的变化特征,结合土壤酶活性,探究不同施肥处理下驱动细菌、真菌群落变化的关键土壤环境因子。结果表明,T1、T2处理显著提高了土壤纤维素酶、蔗糖酶的活性,T3、T4、T5处理显著提高了脲酶、酸性磷酸酶的活性,T2处理显著提高了过氧化氢酶的活性;各处理的细菌Shannon指数、Simpson指数均显著高于CK,T1、T3处理的Chao1指数、ACE指数显著高于CK;主成分分析结果表明,土壤真菌群落结构在处理间达到显著性差异,CK与T1及T3处理、CK与T2及T4处理分别在土壤细菌、真菌群落结构上差异最大;变形菌门、放线菌门、酸杆菌门、绿湾菌门均为土壤的优势细菌门,子囊菌门、担子菌门、未确定分类真菌门、壶菌门为优势真菌门;Spearman相关热图显示,土壤过氧化氢酶活性更易影响根际土壤真菌的相对丰度及多样性。综合而言,T2、T4处理更有利于提高土壤酶的活性,丰富微生物群落的多样性,并可改变微生物群落的结构,为本研究中芒果的最佳施肥方式。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2023-12-19
基金项目:广西创新驱动发展专项资金(编号:桂科AA22068098);百色市科技局项目(编号:20211825-2);百色学院科研项目(编号:2019KN);大学生创新创业训练计划(编号:S202210609182)。
作者简介:汪自松(1977—),男,湖北鄂州人,博士,助理研究员,主要从事植物营养学研究。E-mail:1006139505@qq.com。
通信作者:刘正鲁,博士,副教授,主要从事蔬菜营养学研究,E-mail:371737184@qq.com;赵志常,博士,副研究员,主要从事热带果树生物学研究,E-mail:longanzz@126.com。
更新日期/Last Update: 2025-01-20