|本期目录/Table of Contents|

[1]李孟军,张思尚,李娜娜,等.高效纳米硒合成菌Bacillus licheniformis ZY3的分离、鉴定及其产吲哚乙酸能力[J].江苏农业科学,2025,53(3):215-222.
 Li Mengjun,et al.Isolation and identification of efficient nano selenium synthesis bacterium Bacillus licheniformis ZY3 and its ability to produce indoleacetic acid[J].Jiangsu Agricultural Sciences,2025,53(3):215-222.
点击复制

高效纳米硒合成菌Bacillus licheniformis ZY3的分离、鉴定及其产吲哚乙酸能力(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第3期
页码:
215-222
栏目:
资源与环境
出版日期:
2025-02-05

文章信息/Info

Title:
Isolation and identification of efficient nano selenium synthesis bacterium Bacillus licheniformis ZY3 and its ability to produce indoleacetic acid
作者:
李孟军1 张思尚1 李娜娜1 何嘉丽1 龚珏3 王璋倩12 何毅12 程水源12
1.武汉轻工大学硒科学与工程现代产业学院,湖北武汉 430048; 2.国家富硒农产品加工技术研发专业中心,湖北武汉 430048; 3.湖北国硒科技发展有限公司,湖北恩施 445000
Author(s):
Li Mengjunet al
关键词:
地衣芽孢杆菌耐硒纳米硒吲哚乙酸富硒促生菌
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
从富硒土壤中筛选出1株高耐硒菌株,通过形态学观察和分子生物学分析,对其菌种进行鉴定,并作生长还原动力学分析,对其纳米硒生成率、还原生成纳米硒的表征及其产生长素吲哚乙酸的能力进行研究。结果表明,菌株ZY3为地衣芽孢杆菌(Bacillus licheniformis),最高可耐受400 mmol/L亚硒酸钠。菌株ZY3的纳米硒生成能力较强,能够在16 h内将5 mmol/L亚硒酸钠95.5%还原为红色纳米硒;纯化后的纳米硒粒径为(158.8±1.82) nm,Zeta电位为 -31.79 mV,稳定性高。菌株ZY3具有较强的产吲哚乙酸能力,48 h内分泌量为47.15 mg/L;添加100 mg/L色氨酸后,吲哚乙酸分泌量提升1.43倍。高产纳米硒的吲哚乙酸产生菌可用于生产富硒微生物肥料。期待本研究结果可为研发富硒微生物肥料和富硒农产品提供理论依据。
Abstract:
-

参考文献/References:

[1]Huang S S,Yu K,Wen L,et al. Development and application of a new biological nano-selenium fermentation broth based on Bacillus subtilis SE201412[J]. Scientific Reports,2023,13:2560.
[2]Yang X Q,Fu Y,Zhang J B,et al. Preparation,characterization,and antioxidant and antiapoptotic activities of biosynthesized nano-selenium by yak-derived Bacillus cereus and chitosan-encapsulated chemically synthesized nano-selenium[J]. International Journal of Biological Macromolecules,2023,242(Pt 1):124708.
[3]Rayman M P. Selenium and human health[J]. Lancet,2012,379(9822):1256-1268.
[4]胡隆庆,钱贝,邴凯健,等. 我国硒的环境分布及其与甲状腺疾病关系研究[J]. 安全与环境工程,2022,29(5):13-21.
[5]Shi Z M,Pan P J,Feng Y W,et al. Environmental water chemistry and possible correlation with Kaschin-Beck Disease (KBD) in northwestern Sichuan,China[J]. Environment International,2017,99:282-292.
[6]杨锐,余雍和,程水源,等. 耐硒芽孢杆菌的筛选及其亚硒酸盐还原机制的探究[J]. 食品工业科技,2021,42(22):105-111.
[7]范书伶,王平,张珩琳,等. 环境中硒的迁移、微生物转化及纳米硒应用研究进展[J]. 科学通报,2020,65(26):2853-2862.
[8]王金泽,李静,郭耀东,等. 富硒乳酸菌亚硒酸盐还原特性及产硒机理研究[J]. 粮食与油脂,2022,35(4):139-144.
[9]张晨婷. 解淀粉芽孢杆菌制备纳米硒的条件优化及分子机制初探[D]. 山西:山西农业大学,2023.
[10]Ullah A,Yin X,Wang F H,et al. Biosynthesis of selenium nanoparticles (via Bacillus subtilis BSN313),and their isolation,characterization,and bioactivities[J]. Molecules,2021,26(18):5559.
[11]任文义,程雨辰,何金童,等. 地衣芽孢杆菌的生物学功能及其作为青贮饲料添加剂的应用潜力[J]. 动物营养学报,2023,35(10):6269-6276.
[12]王一丹,余雍和,杨锐,等. 耐硒菌株Cytobacillus firmus N4的筛选及其对α-葡萄糖苷酶抑制活性的影响[J]. 食品与生物技术学报,2023,42(2):45-50.
[13]高义霞,周向军,王亮亮,等. 纳米硒微肥的形貌分析及对豌豆幼苗生长的影响[J]. 黑龙江农业科学,2023(4):24-30.
[14]孙鹏波,王志军,格根图,等. 喷施纳米硒对紫花苜蓿产量、营养品质和硒含量的影响[J]. 中国草地学报,2023,45(8):79-87.
[15]Radhakrishnan R,Hashem A,Abd Allah E F. Bacillus:a biological tool for crop improvement through bio-molecular changes in adverse environments[J]. Frontiers in Physiology,2017,8:667.
[16]马丽莎,陈小玲,檀艳萍,等. 一株高耐硒雷氏普罗威登斯菌的分离鉴定[J]. 食品科技,2023,48(5):1-9.
[17]张立秋,向建伟,胡琴,等. 利用地衣芽孢杆菌制备纳米硒能力的研究[J]. 山东化工,2023,52(8):13-16,20.
[18]郑世学,姚蓉,杨丽琛,等. 芽孢杆菌ES2-45高效产纳米硒并应用于小白菜富硒[C]//中国环境科学学会,中南大学,中南林业科技大学,湖南农业大学,第十届重金属污染防治技术及风险评价研讨会论文集. 长沙:中国环境科学学会,2020:1.
[19]Abd-Alla M H,El-Sayed E S A,Rasmey A H M. Indole-3-acetic acid (IAA) production by Streptomyces atrovirens isolated from rhizospheric soil in Egypt[J]. Journal Biology and Earth Science,2013,3(2):182-193.
[20]Sokol N W,Slessarev E,Marschmann G L,et al. Life and death in the soil microbiome:how ecological processes influence biogeochemistry[J]. Nature Reviews Microbiology,2022,20:415-430.
[21]Sarmiento-López L G,López-Meyer M,Maldonado-Mendoza I E,et al. Production of indole-3-acetic acid by Bacillus circulans E9 in a low-cost medium in a bioreactor[J]. Journal of Bioscience and Bioengineering,2022,134(1):21-28.
[22]东秀珠,蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社,2001.
[23]Loeschner K,Hadrup N,Hansen M,et al. Absorption,distribution,metabolism and excretion of selenium following oral administration of elemental selenium nanoparticles or selenite in rats[J]. Metallomics,2014,6(2):330-337.
[24]廖延雄.《伯杰氏鉴定细菌学手册》与《伯杰氏分类细菌学手册》[J]. 微生物学通报,1992(4):249.
[25]Avendao R,Chaves N,Fuentes P,et al. Production of selenium nanoparticles in Pseudomonas putida KT2440[J]. Scientific Reports,2016,6:37155.
[26]袁永强,朱建明,刘丛强,等. 高硒碳质泥岩中的3株高还原耐受亚硒酸盐菌[J]. 地学前缘,2014,21(2):331-341.
[27]Larsen E H,Lobinski R,Burger-Meer K,et al. Uptake and speciation of selenium in garlic cultivated in soil amended with symbiotic fungi (mycorrhiza) and selenate[J]. Analytical and Bioanalytical Chemistry,2006,385(6):1098-1108.
[28]Duan Y H,Li M J,Zhang S S,et al. Highly efficient biotransformation and production of selenium nanoparticles and polysaccharides using potential probiotic Bacillus subtilis T5[J]. Metabolites,2022,12(12):1204.
[29]Wang Z,Li N,Zhou X,et al.Optimization of fermentation parameters to improve the biosynthesis of selenium nanoparticles by Bacillus licheniformis F1 and its comprehensive application[J]. BMC Microbiology,2024,24-271.
[30]Altschul S F,Gish W,Miller W,et al. Basic local alignment search tool[J]. Journal of Molecular Biology,1990,215(3):403-410.
[31]Wang Y D,Yu Y H,Duan Y H,et al. Enhancing the activity of carboxymethyl cellulase enzyme using highly stable selenium nanoparticles biosynthesized by Bacillus paralicheniformis Y4[J]. Molecules,2022,27(14):4585.
[32]Ashengroph M,Hosseini S R. A newly isolated Bacillus amyloliquefaciens SRB04 for the synthesis of selenium nanoparticles with potential antibacterial properties[J]. International Microbiology,2021,24(1):103-114.
[33]Wang Y T,Shu X,Hou J Y,et al. Selenium nanoparticle synthesized by Proteus mirabilis YC801:an efficacious pathway for selenite biotransformation and detoxification[J]. International Journal of Molecular Sciences,2018,19(12):3809.
[34]Eszenyi P,Sztrik A,Babka B,et al. Elemental,nano-sized (100~500 nm) selenium production by probiotic lactic acid bacteria[J]. International Journal of Bioscience,Biochemistry and Bioinformatics,2011:148-152.
[35]Zhu Y Y,Ren B Y,Li H F,et al. Biosynthesis of selenium nanoparticles and effects of selenite,selenate,and selenomethionine on cell growth and morphology in Rahnella aquatilis HX2[J]. Applied Microbiology and Biotechnology,2018,102(14):6191-6205.
[36]王露. 产吲哚乙酸细菌筛选优化及对番茄幼苗生长的影响[D]. 哈尔滨:东北农业大学,2023.
[37]董颐玮,朱燕云,靳红梅. 生物纳米硒对杭白菜品质与抗氧化性能的影响[J]. 江苏农业学报,2023,39(2):479-488.

相似文献/References:

[1]史经略,梁天林.生物发酵猪饲料饲喂仔猪的效果[J].江苏农业科学,2013,41(12):218.
 Shi Jinglue,et al.Feeding effect of fermented forage on pigs[J].Jiangsu Agricultural Sciences,2013,41(3):218.
[2]陈丽丽,何玲玲,赵雅,等.地衣芽孢杆菌W10对烟草的促生作用及机制[J].江苏农业科学,2016,44(05):152.
 Chen Lili,et al.Growth-promoting effect and mechanism of Bacillus licheniformis W10 on tobacco[J].Jiangsu Agricultural Sciences,2016,44(3):152.
[3]方光远,茅慧华,郭宇飞,等.分解鸽羽毛地衣芽孢杆菌的分离与鉴定[J].江苏农业科学,2015,43(12):387.
 Fang Guangyuan,et al.Isolation and identification of Bacillus licheniformis for decomposition of pigeon feathers[J].Jiangsu Agricultural Sciences,2015,43(3):387.
[4]葛文霞,柳旭伟.地衣芽孢杆菌对黄羽肉鸡生产性能和血液生化指标的影响[J].江苏农业科学,2015,43(07):213.
 Ge Wenxia,et al.Effects of Bacillus licheniformis on growth performance and biochemical parameters of yellow-feathered broilers[J].Jiangsu Agricultural Sciences,2015,43(3):213.
[5]杭小英,周冬仁,罗毅志,等.地衣芽孢杆菌的生长及对养殖水体中残饵的降解特性[J].江苏农业科学,2015,43(03):206.
 Hang Xiaoying,et al.Growth of Bacillus licheniformis and its degradation to residual bait in aquaculture water[J].Jiangsu Agricultural Sciences,2015,43(3):206.
[6]林标声,吴江文,江火香,等.适合银杏叶发酵的菌株筛选及发酵效果[J].江苏农业科学,2016,44(04):315.
 Lin Biaosheng,et al.Screening of strains suitable for ginkgo leaf fermentation and its fermentation effect[J].Jiangsu Agricultural Sciences,2016,44(3):315.
[7]陈切希,简志青,贾冬英,等.地衣芽孢杆菌B-1和鞘氨醇单胞菌SC-1原生质体制备与再生条件的研究[J].江苏农业科学,2018,46(05):36.
 Chen Qiexi,et al.Study on preparation and regenerated factors of protoplasts from Bacillus licheniformis B-1 and Sphingomonas sp. SC-1[J].Jiangsu Agricultural Sciences,2018,46(3):36.

备注/Memo

备注/Memo:
收稿日期:2023-12-20
基金项目:湖北省自然科学基金(编号:2023AFB968);武汉轻工大学博士科研启动基金(编号:2023RZ015);湖北省乡村振兴科技支撑项目(编号:2022BBA114)。
作者简介:李孟军(1997—),女,河南周口人,硕士研究生,主要研究方向为微生物学。E-mail:limengjun202102@163.com。
通信作者:王璋倩,博士,副教授,主要从事资源与应用微生物学及天然产物研发研究,E-mail:wzqsnu@whpu.edu.cn;何毅,博士,副教授,主要从事食品生物技术研究,E-mail:heyi629@126.com。
更新日期/Last Update: 2025-02-05