[1]刘凌云,王东泽,才满,等.美洲黑杨JAZ基因家族鉴定及其对害虫取食的表达响应[J].江苏农业科学,2025,53(4):73-82.
 Liu Lingyun,et al.Identification of JAZ gene family in Populus deltoides and their expression response to pest feeding[J].Jiangsu Agricultural Sciences,2025,53(4):73-82.
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美洲黑杨JAZ基因家族鉴定及其对害虫取食的表达响应()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第4期
页码:
73-82
栏目:
抗虫基因
出版日期:
2025-02-20

文章信息/Info

Title:
Identification of JAZ gene family in Populus deltoides and their expression response to pest feeding
作者:
刘凌云12王东泽12才满12李中强3顾丽姣12
1.河北农业大学林学院,河北保定 071000; 2.河北省林木种质资源与森林保护重点实验室,河北保定 071000; 3.河北华测检测服务有限公司,河北石家庄 050000
Author(s):
Liu Lingyunet al
关键词:
美洲黑杨PdJAZ基因家族生信分析生物胁迫
Keywords:
-
分类号:
S792.110.1
DOI:
-
文献标志码:
A
摘要:
JAZ(jasmonate ZIM-domain,JAZ)作为植物特有的基因家族,参与植物生长发育、生物胁迫、非生物胁迫、激素调控等多个途径。为了探究美洲黑杨(Populus deltoides)JAZ基因家族的特征并预测其功能,本研究对美洲黑杨JAZ基因家族进行了全基因组鉴定和分析。结果表明:在美洲黑杨基因组中共鉴定出13个PdJAZ家族蛋白,划分为3个亚组。所有PdJAZ蛋白都具有ZIM和Jas结构域,并且除PdJAZ8存在于细胞膜和细胞核中其余均位于细胞核中。对筛选出的 13 个 PdJAZ基因对应的蛋白进行理化性质、二(三)级结构分析发现,该家族成员理化性质上具有差异,它们的氨基酸个数在134~475个不等,分子量在1.5~4.1 ku之间,PdJAZ等电点均大于7且不超过10。13个基因的二级结构以α-螺旋和无规则卷曲为主,β-转角占比极小。物种内共线性分析显示,PdJAZ基因家族存在7个共线性基因对,PdJAZ9基因不存在共线性关系。motif和基因结构分析显示,同一分支上的motif组成和内含子外显子结构相似,不同分支上的差异较大,说明PdJAZ蛋白在功能上应该具有一定的多样性和保守性。启动子顺式作用元件分析显示,启动子区域含有多种植物激素和非生物响应元件,其中脱落酸是数量最多的元件。此外,美洲黑杨PdJAZ基因在虫害胁迫下根据取食量的不同表现出不同的表达模式,这表明PdJAZ可能在不同程度上参与调控美洲黑杨的抗虫性。
Abstract:
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相似文献/References:

备注/Memo

备注/Memo:
收稿日期:2024-09-05
基金项目:河北省自然科学基金(编号:C2022204047、C2022204115);国家自然科学基金(编号:32201578);河北省重点研发计划(编号:22326318D)。
作者简介:刘凌云(2000—),女,贵州遵义人,硕士研究生,研究方向为林木遗传育种。E-mail:1609425419@qq.com。
通信作者:顾丽姣,博士,讲师,研究方向为林木遗传育种。E-mail:gulijiao1990@126.com。
更新日期/Last Update: 2025-02-20