[1]王东,张超,周洪友,等.贝莱斯芽孢杆菌CMC6在秸秆降解及堆肥中的应用[J].江苏农业科学,2026,54(5):241-247.
 Wang Dong,et al.Application of Bacillus velezensis CMC6 in straw degradation and compost[J].Jiangsu Agricultural Sciences,2026,54(5):241-247.
点击复制

贝莱斯芽孢杆菌CMC6在秸秆降解及堆肥中的应用()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第5期
页码:
241-247
栏目:
农业资源与环境
出版日期:
2026-03-05

文章信息/Info

Title:
Application of Bacillus velezensis CMC6 in straw degradation and compost
作者:
王东13张超123周洪友13牟英男2赵明敏13余江泳13柴全贤4; 赵远征12
1.内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特 010018; 2.内蒙古自治区农牧业科学院植物保护研究所,内蒙古呼和浩特 010031; 3.内蒙古农业大学生物农药创制与资源利用重点实验室,内蒙古呼和浩特 010018; 4.阿拉善左旗沙产业开发管理有限公司,内蒙古阿拉善盟 750306
Author(s):
Wang Donget al
关键词:
秸秆纤维素酶降解贝莱斯芽孢杆菌堆肥发酵
Keywords:
-
分类号:
S141.4;S182
DOI:
-
文献标志码:
A
摘要:
为解决内蒙古自治区养殖业粪便和农田秸秆废弃物降解困难及环境污染等问题,利用刚果红染色法、苯胺蓝脱色法和滤纸条崩解法对玉米秸秆中筛选得到的贝莱斯芽孢杆菌CMC-6进行降解功能测定,通过室内降解试验、纤维素酶活性测定和扫描电镜观察验证菌株CMC-6降解能力,同时将其接种堆肥中并测定有机质含量、全氮含量等堆体理化性质,最后用小麦盆栽试验验证应用效果。结果显示,菌株CMC-6刚果红水解值为7.78,苯胺蓝脱色圈直径达3.6 cm,对滤纸条具有较强的降解能力;接种该菌株液态发酵30 d后秸秆降解率达到55.55%,纤维素酶活性在发酵4 d后达到最大值,为216.06 U/mL,同时扫描电镜观察发现其处理后的玉米秸秆表面崩解、断裂。在堆肥中接种该菌株发酵液4 d后,堆体发酵中心温度达到47.5 ℃,累积高温期达到8 d以上,接种菌株的堆体在27 d后含水量下降20.35%,有机质含量下降27.10%,碳氮比(C/N)降至17.69,全氮含量升高27.17%。发酵液处理组的小麦种子发芽指数显著升高,其根长、株高、鲜重分别较对照组提高64.28%、52.21%和82.24%。综上,贝莱斯芽孢杆菌CMC-6具有较强的秸秆降解能力,在缩短堆肥发酵周期的同时提升堆肥发酵质量。
Abstract:
-

参考文献/References:

[1]He Y W,He Y D,Al-Dhabi N A,et al. Effects of exogenous thermophilic bacteria and ripening agent on greenhouse gas emissions,enzyme activity and microbial community during straw composting[J]. Bioresource Technology,2024,407:131114.
[2]张旭屹,彭仕乐,谢慧,等. 固氮与秸秆降解复合菌剂构建及其在秸秆还田中的应用[J]. 河南农业大学学报,2025,59(2):306-315.
[3]于博,刘雅梦,杨哲,等. 内蒙古平原灌区土壤固碳与养分吸附特征及秸秆还田调控[J]. 华北农学报,2024,39(1):120-126.
[4]严东权,薛颖昊,徐志宇,等. 我国农作物秸秆直接还田利用现状、技术模式及发展建议[J]. 中国农业资源与区划,2023,44(4):1-14.
[5]陈云峰,夏贤格,杨利,等. 秸秆还田是秸秆资源化利用的现实途径[J]. 中国土壤与肥料,2020(6):299-307.
[6]张超. 多功能秸秆降解菌的筛选与应用[D]. 呼和浩特:内蒙古农业大学,2024.
[7]韩大勇,李拥军,刘海霞,等. 稻秸秆对羊粪好氧堆肥理化特性与氮素转化的影响[J]. 农业环境科学学报,2024,43(8):1896-1906.
[8]Yang X Y,Duan P P,Liu Q M,et al. Addition of cellulose and hemicellulose degrading microorganisms intensified nitrous oxide emission during composting[J]. Bioresource Technology,2024,393:130100.
[9]Chen S M,Fan Z W,Li Y Y,et al. Straw additive enhances manure compost quality by promoting diverse aerobic bacteria and unitary thermophilic fungi[J]. Environmental Technology & Innovation,2024,36:103818.
[10]Chen S F,Huang H L,Zhang J C,et al. Effects of composite microbial agent with straw or sawdust on organic matter degradation,enzyme activity and functional genes during aerobic composting of food waste[J]. Process Biochemistry,2024,146:423-432.
[11]Zhang Y H,Li H,Zhang M Q,et al. Whole genome sequencing analysis of cellulose-degrading Bacterium DC11 isolated from silkworm excrement and characterization of its key cellulase gene ytoP running head:characterization of strain DC11 and its key gene ytoP[J]. Journal of Asia-Pacific Entomology,2024,27(3):102285.
[12]Xu C F,Su X,Wang J H,et al. Characteristics and functional bacteria in a microbial consortium for rice straw lignin-degrading[J]. Bioresource Technology,2021,331:125066.
[13]Wang J H,Zhang W,Wu C Y,et al. Synergistic analysis of lignin degrading bacterial consortium and its application in rice straw fiber film[J]. Science of the Total Environment,2024,927:172386.
[14]陈俊文,刘庆华,庞学勇. 一株厚壁隔孢伏革菌的筛选及木质纤维素降解特性[J]. 西南农业学报,2023,36(11):2491-2499.
[15]宋雨,白红娟,胡锦俊,等. 秸秆纤维素降解菌的分离筛选、复合菌系构建及产酶条件优化[J]. 中国酿造,2023,42(2):108-114.
[16]李红霞,蔡禄,季祥,等. 羊粪好氧堆肥最佳工艺参数的优化研究[J]. 中国农机化学报,2019,40(6):215-220.
[17]黄悦姝,邓超,张馨月,等. 贝莱斯芽孢杆菌D103产纤维素酶优化及途径分析[J]. 生物技术,2023,33(5):632-640.
[18]王文凡,刘银秀,谢晓杰,等. 牛粪堆肥中纤维素高效降解菌的筛选与产酶条件优化[J]. 微生物学通报,2023,50(11):4796-4811.
[19]郭琪,刘瑞,徐刚,等. 1株水稻秸秆降解菌的筛选及其降解效果研究[J]. 西北农林科技大学学报(自然科学版),2025,53(3):102-112.
[20]段袁慧真. 秸秆覆盖对油茶-决明子间作土壤酶活性及微生物群落的影响[D]. 长沙:中南林业科技大学,2023.
[21]赵远征,张超,王东,等. 贝莱斯芽孢杆菌及其在农业生产中的应用:CN118995538B[P]. 2025-01-28.
[22]张颖. 耐盐碱促生微生物的筛选、鉴定与应用初探[D]. 呼和浩特:内蒙古农业大学,2023.
[23]张金辉,李银月,张发文,等. 物料碳氮比及微生物菌剂接种量对黄瓜秧-鸡粪堆肥过程的影响[J]. 江苏农业学报,2024,40(2):260-269.
[24]杨慧珍. 碳氮比对好氧堆肥过程中有机碳转化的影响机制研究[D]. 兰州:甘肃农业大学,2022.
[25]Zhao B H,Dong W J,Chen Z L,et al. Microbial inoculation accelerates rice straw decomposition by reshaping structure and function of lignocellulose-degrading microbial consortia in paddy fields[J]. Bioresource Technology,2024,413:131545.
[26]Mei J F,Shen X B,Gang L P,et al. A novel lignin degradation bacteria—Bacillus amyloliquefaciens SL-7 used to degrade straw lignin efficiently[J]. Bioresource Technology,2020,310:123445.
[27]戴相群. 秸秆降解菌的筛选及全程有机植保体系的推广[D]. 南京:南京农业大学,2016.
[28]Thomas L,Ram H,Kumar A,et al. Production,optimization,and characterization of organic solvent tolerant cellulases from a lignocellulosic waste-degrading actinobacterium,Promicromonospora sp.VP111[J]. Applied Biochemistry and Biotechnology,2016,179(5):863-879.
[29]Xu J,Yang Q. Isolation and characterization of rice straw degrading Streptomyces griseorubens C-5[J]. Biodegradation,2010,21(1):107-116.
[30]陈忠男,王志刚,徐伟慧. 贝莱斯芽孢杆菌WB对西瓜植株的促生效应和机制[J]. 农业生物技术学报,2024,32(10):2228-2242.
[31]司海丽,纪立东,李晓慧,等. 纤维素降解菌的筛选、鉴定及其秸秆降解效果[J]. 江苏农业科学,2024,52(18):277-283.
[32]Wang Z Y,Wang R X,Zhou J S,et al. An assessment of the genomics,comparative genomics and cellulose degradation potential of Mucilaginibacter polytrichastri strain RG4-7[J]. Bioresource Technology,2020,297:122389.
[33]曹平华,李晓霞,李旺,等. 1株烟曲霉产耐热纤维素酶发酵条件优化及酶学性质研究[J]. 中国畜牧兽医,2023,50(11):4414-4423.
[34]张慧,赵恪,林陈强,等. 纤维素降解菌的分离筛选及产酶条件优化[J]. 福建农业学报,2023,38(9):1117-1123.
[35]Yao X F,Liu Q M,Li D J. Mechanism underlying effects of cellulose-degrading microbial inoculation on amino acid degradation and biosynthesis during composting[J]. Bioresource Technology,2024,403:130899.
[36]徐鹏,徐晓东,李洋,等. 木质纤维素降解菌在秸秆堆肥中的研究现状与进展[J]. 北方园艺,2022(8):122-128.
[37]刘林培,管秀琼,王洪,等. 食用菌菌渣和白酒丢糟共堆肥过程中氮素变化及腐熟进程[J]. 食品与发酵工业,2020,46(21):188-194.
[38]王献鑫,祁丽桦,宋江富,等. 纤维素、木质素降解菌的筛选及对菌渣堆肥的影响[J]. 河南农业科学,2024,53(9):66-79.
[39]Wang X G,Tian L,Li Y X,et al. Effects of exogenous cellulose-degrading bacteria on humus formation and bacterial community stability during composting[J]. Bioresource Technology,2022,359:127458.

相似文献/References:

[1]温钰娟,申哲民,董宇,等.农作物秸秆水热糖化及其残渣的综合利用[J].江苏农业科学,2013,41(05):269.
 Wen Yujuan,et al.Hydrothermal saccharification of crop stalks and comprehensive utilization of their scraps[J].Jiangsu Agricultural Sciences,2013,41(5):269.
[2]牛明芬,于海娇,武肖媛,等.猪粪秸秆高温堆肥过程中物质变化的研究[J].江苏农业科学,2014,42(09):291.
 Niu Mingfen,et al.Study on material changes during process of pig manure and straw composting[J].Jiangsu Agricultural Sciences,2014,42(5):291.
[3]王丽学,李振鹏,刘四平,等.玉米在不同覆盖方式处理下的土壤水温差异[J].江苏农业科学,2016,44(03):82.
 Wang Lixue,et al.Differences of soil moisture and temperature under different mulching treatments of maize[J].Jiangsu Agricultural Sciences,2016,44(5):82.
[4]刘源,陈功磊,郑网宇,等.不同来源养分对稻田施肥周期内田面水氮含量的影响[J].江苏农业科学,2016,44(03):381.
 Liu Yuan,et al.Effects of different exogenous nutrients input on nitrogen concentration of surface water in a paddy field during fertilization period[J].Jiangsu Agricultural Sciences,2016,44(5):381.
[5]明哲.秸秆粉碎压块机的结构和变速器的设计[J].江苏农业科学,2013,41(11):409.
 Ming Zhe.Structure of straw crushing and pressing machine and design of transmission[J].Jiangsu Agricultural Sciences,2013,41(5):409.
[6]周玲玲,张黎杰,姜若勇,等.不同墙体材料的日光温室环境变化及其对辣椒产量与品质的影响[J].江苏农业科学,2014,42(02):339.
 Zhou Lingling,et al.Effects of different materials of greenhouse walls on environmental factors and yield and quality of pepper[J].Jiangsu Agricultural Sciences,2014,42(5):339.
[7]张文静,肖佳冰,李佳,等.秸秆覆盖和保水剂对烤烟化学成分的影响[J].江苏农业科学,2016,44(05):155.
 Zhang Wenjing,et al.Effects of straw and water-retaining agent on chemical components of flue-cured tobacco[J].Jiangsu Agricultural Sciences,2016,44(5):155.
[8]黄周满,牛雯婧.秸秆/污泥制备活性炭及处理生活污水效果[J].江苏农业科学,2016,44(05):435.
 Huang Zhouman,et al.Preparation of activated carbon by straw/sludge and its treating effect on sewage[J].Jiangsu Agricultural Sciences,2016,44(5):435.
[9]李雪,张欣,葛长明,等.不同秸秆厌氧发酵产沼气潜力研究[J].江苏农业科学,2016,44(06):496.
 Li Xue,et al.Study on biogas production performance of different straw by anaerobic digestion[J].Jiangsu Agricultural Sciences,2016,44(5):496.
[10]陈智文,李天祺,秦旭升,等.吉林省黑钙土农田土壤养分时间变化特征[J].江苏农业科学,2016,44(02):344.
 Chen Zhiwen,et al.Temporal variation characteristics of soil nutrients in chernozem of Jilin Province[J].Jiangsu Agricultural Sciences,2016,44(5):344.

备注/Memo

备注/Memo:
收稿日期:2025-04-16
基金项目:内蒙古中央引导地方发展基金(编号:2024ZY0028);内蒙古自治区直属高校基本科研业务费(编号:BR22-11-14);呼和浩特市科技计划项目(编号:2023-社-3)。
作者简介:王东(1985—),男,河北张家口人,博士,副教授,研究方向为植物微生态制剂。E-mail:wangdong@imau.edu.cn。
通信作者:赵远征,博士,副研究员,研究方向为功能微生物资源收集与利用。E-mail:zhaoyuanzheng.gege@163.com。
更新日期/Last Update: 2026-03-05