|本期目录/Table of Contents|

[1]王志强,俞红贤,荆海霞,等.牦牛SLC25A6基因的CDS序列及其表达蛋白生物信息学分析[J].江苏农业科学,2014,42(09):36-39.
　Wang Zhiqiang,et al.CDS cloning and expression of SLC25A6 gene and its bioinformatics analysis in yak[J].Jiangsu Agricultural Sciences,2014,42(09):36-39.
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牦牛SLC25A6基因的CDS序列及其表达蛋白生物信息学分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年09期
页码:
36-39
栏目:
生物技术
出版日期:
2014-09-25

文章信息/Info

Title:
CDS cloning and expression of SLC25A6 gene and its bioinformatics analysis in yak
作者:
王志强;  俞红贤;  荆海霞;  张勤文;  魏青;  梁林;  牛亮
青海大学，青海西宁 810016
Author(s):
Wang Zhiqiang; et al
关键词:
牦牛; 线粒体; SLC25A6基因; ANT3蛋白; 生物信息学
Keywords:
-
分类号:
Q753
DOI:
-
文献标志码:
A
摘要:
通过RT-PCR技术克隆牦牛SLC25A6基因的cDNA序列，并利用DNAMAN、MAGA 6、SWISS-MODEL、ExPASy 等生物信息学软件系统对其进行分析。结果表明，扩增出的牦牛SLC25A6基因的编码区长897 bp，编码298个氨基酸；与普通牛、绵羊和人的相应基因核苷酸序列进行比对，序列一致性分别为99.33%、98.22%、91.86%；其编码蛋白分子量为32.821 ku，含多个修饰位点。
Abstract:
-

参考文献/References:

［1］Pebay-Peyroula E，Dahout-Gonzalez C，Kahn R，et al.Structure of mitochondrial ADP/ATP carrier in complex with carboxyatractyloside［J］. Nature，2003，426(6962)：39-44.
［2］Visser W F，van Roermund C W T，Waterham H R，et al. Identification of human PMP34 as a peroxisomal ATP transporter［J］. Biochemical and Biophysical Research Communications，2002，299(3)：494-497.
［3］Lawson J E，Gawaz M，Klingenberg M，et al. Structure-function studies of adenine nucleotide transport in mitochondria. Ⅰ. Construction and genetic analysis of yeast mutants encoding the ADP/ATP carrier protein of mitochondria［J］. The Journal of Biological Chemistry，1990，265(24)：14195-14201.
［4］Jang J Y，Lee C E. Mitochondrial adenine nucleotide translocator 3 is regulated by IL-4 and IFN-gamma via STAT-dependent pathways［J］. Cell Immunol，2003，226(1)：11-19.
［5］Jang J Y，Lee C E. IL-4-induced upregulation of adenine nucleotide translocase 3 and its role in Th cell survival from apoptosis［J］. Cellular Immunology，2006，241(1)：14-25.
［6］Zamora M，Granell M，Mampel T，et al. Adenine nucleotide translocase 3(ANT3) overexpression induces apoptosis in cultured cells［J］. FEBS Letters，2004，563(1/2/3)：155-160.
［7］Arnold K，Bordoli L，Kopp J，et al. The SWISS-MODEL workspace：a web-based environment for protein structure homology modelling［J］. Bioinformatics，2006，22(2)：195-201.
［8］Benkert P，Biasini M，Schwede T. Toward the estimation of the absolute quality of individual protein structure models［J］. Bioinformatics，2011，27(3)：343-350.
［9］Saraste M，Walker J E. Internal sequence repeats and the path of polypeptide in mitochondrial ADP/ATP translocase［J］. FEBS Letters，1982，144(2)：250-254.
［10］Kunji E R S.The role and structure of mitochondrial carriers［J］. FEBS Letters，2004，564(3)：239-244.

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备注/Memo

备注/Memo:
收稿日期：2014-03-19
基金项目：国家自然科学基金(编号：31260588)；青海省自然科学基金(编号：2011-Z-901)。
作者简介：王志强(1988—)，男，河南焦作人，硕士研究生，主要从事高原家畜形态学结构特点与高原环境的关系研究。E-mail：wzqqhu@163.com。
通信作者：荆海霞，博士，副教授，主要从事高原家畜形态学结构特点与高原环境的关系研究。E-mail：jinghaixia1@163.com。
更新日期/Last Update: 2014-09-25