|本期目录/Table of Contents|

[1]李范,李娜,陈建中,等.基于磷脂脂肪酸提取方法的微生物群落结构研究[J].江苏农业科学,2014,42(09):323-325.
　Li Fan,et al.Study on microbial community structure based on phospholipid fatty acid extraction method[J].Jiangsu Agricultural Sciences,2014,42(09):323-325.
点击复制

基于磷脂脂肪酸提取方法的微生物群落结构研究(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年09期
页码:
323-325
栏目:
资源与环境
出版日期:
2014-09-25

文章信息/Info

Title:
Study on microbial community structure based on phospholipid fatty acid extraction method
作者:
李范1;  李娜2;  陈建中2;  李萍1;  陈怡2;  蒋小强2
1.西南交通大学生命科学与工程学院，四川成都 610031； 2.中国科学院成都生物研究所，四川成都 610041
Author(s):
Li Fan; et al
关键词:
土壤; 凋落物; PLFA(磷脂脂肪酸); 微生物群落结构
Keywords:
-
分类号:
S154.3
DOI:
-
文献标志码:
A
摘要:
选取川西高原土壤和凋落物作为研究对象，分别取1、3、5、8、10 g土壤和0.5、0.7、0.9、1.0、1.2 g凋落物用于PLFA(磷脂脂肪酸)的提取。经试验确定，土壤和凋落物用于PLFA提取的最佳提取量分别为8 g和1.0 g，用超声波提取替代振荡提取，缩短了PLFA方法的提取时间。进一步分析土壤和凋落物中微生物群落结构信息，结果表明，相同环境下土壤和凋落物中大部分微生物类群的种类和含量都十分接近，革兰氏阳性菌比革兰氏阴性菌更有优势，厌氧菌比好养菌更加活跃。
Abstract:
-

参考文献/References:

［1］Bligh E G，Dyer W J. A rapid method of 总 lipid extraction and purification［J］. Canadian Journal of Biochemistry and Physiology，1959，37(8)：911-917.
［2］Brant J B，Myrold D D，Sulzman E W. Root controls on soil microbial community structure in forest soils［J］. Oecologia，2006，148(4)：650-659.
［3］Bai Q，Gattinger A，Zelles L. Characterization of microbial consortia in paddy rice soil by phospbolipid analysis［J］. Microbial Ecology，2000，39(4)：273-281.
［4］Sakamoto K，Lijima T，Higuchi R. Use of specific phospholipid fatty acids for identifying and quantifying the external hyphae of the arbuscular mycorrhizal fungus Gigaspora rosea［J］. Soil Biology and Biochemistry，2004，36(11)：1827-1834.
［5］Sundh I，Borg P，Nilsson M. et al. Estimation of cell numbers of methanotrophic bacteria in boreal peatlands based on analysis of specific phospholipid fatty acids［J］. FEMS Microbiology Ecology，1995，18(2)：103-112.

相似文献/References:

[1]蒋宝南,刘腾飞,单建明,等.QuEChERS-GC/μECD法测定土壤中的毒死蜱残留量[J].江苏农业科学,2014,42(12):332.
　Jiang Baonan,et al.Determination of chlorpyrifos residues in soil by QuEChERS-GC/μECD[J].Jiangsu Agricultural Sciences,2014,42(09):332.
[2]李国锋,魏瑞成,王冉.高效液相色谱法测定土壤中联苯与间羟基苯甲酸残留[J].江苏农业科学,2014,42(12):316.
　Li Guofeng,et al.Determination of biphenyl and M-hydroxy benzoic acid residues in soil by high performance liquid chromatography[J].Jiangsu Agricultural Sciences,2014,42(09):316.
[3]史景允,于伟红,梁秋生.蓖麻对镉污染土壤的修复潜力[J].江苏农业科学,2014,42(11):386.
　Shi Jingyun,et al(8).Potential repairing of cadmium contaminated soil by castor oil plant[J].Jiangsu Agricultural Sciences,2014,42(09):386.
[4]何继山,梁杏,李静.土样浸提液电导率与盐分关系的逐步回归分析[J].江苏农业科学,2014,42(10):314.
　He Jishan,et al.Regression analysis of relationship between soil samples leaching solution conductivity and solinity[J].Jiangsu Agricultural Sciences,2014,42(09):314.
[5]徐洪文,卢妍.土壤碳矿化及活性有机碳影响因子研究进展[J].江苏农业科学,2014,42(10):4.
　Xu Hongwen,et al.Research progress on soil carbon mineralization and factors affecting active organic carbon[J].Jiangsu Agricultural Sciences,2014,42(09):4.
[6]张乐森,刘悦上,马金芝,等.山东省滨州市设施蔬菜土壤退化防治与修复对策[J].江苏农业科学,2013,41(07):141.
　Zhang Lesen,et al.Control and restoration strategies of facility vegetable soil degradation in Binzhou of Shandong Province[J].Jiangsu Agricultural Sciences,2013,41(09):141.
[7]尹辉,李晖,蒋忠诚,等.典型岩溶区土壤水分的空间异质性研究[J].江苏农业科学,2013,41(07):332.
　Yin Hui,et al.Study on spatial variability of soil water content in typical karst area[J].Jiangsu Agricultural Sciences,2013,41(09):332.
[8]覃怀德,吴炳孙,吴敏,等.橡胶园土壤钾素空间变异与分区管理技术——以海南省琼中县为例[J].江苏农业科学,2013,41(08):326.
　Qin Huaide,et al.Spatial variability and regionalized management of soil potassium nutrient in rubber plantation—Taking Qiongzhong County of Hainan Province as an example[J].Jiangsu Agricultural Sciences,2013,41(09):326.
[9]符勇,周忠发,王昆,等.基于贵州喀斯特高原山区的烟草种植适宜性研究[J].江苏农业科学,2014,42(09):92.
　Fu Yong,et al.Study on planting suitability of tobacco based on Guizhou karst mountain plateau[J].Jiangsu Agricultural Sciences,2014,42(09):92.
[10]李文凤,房翠翠,霍英芝.不同植被类型、海拔高度土壤芽孢杆菌的空间分布特征[J].江苏农业科学,2014,42(08):370.
　Li Wenfeng,et al.Spatial distribution of soil bacillus of different vegetation types and altitudes[J].Jiangsu Agricultural Sciences,2014,42(09):370.

备注/Memo

备注/Memo:
收稿日期：2013-10-30
基金项目：国家自然科学基金 (编号：31170916 )；中国科学院仪器设备功能开发技术创新项目(编号：yg20110708)。
作者简介：李范(1988—)，男，四川自贡人，硕士研究生，主要研究方向为土壤微生物。E-mail：554063409@qq.com。
通信作者：李萍，博士，教授，硕士生导师。E-mail：wuping4535@sina.com。
更新日期/Last Update: 2014-09-25