[1]李葵花,高玉亮,吴京姬.转P5CS基因马铃薯“东农303”耐盐、抗旱性研究[J].江苏农业科学,2014,42(11):131-133.
 Li Kuihua,et al().Study on salt resistance and drought resistance of P5CS transgenic potato cultivar “Dongnong 303”[J].Jiangsu Agricultural Sciences,2014,42(11):131-133.
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转P5CS基因马铃薯“东农303”耐盐、抗旱性研究()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年11期
页码:
131-133
栏目:
育种栽培与生理生化
出版日期:
2014-11-25

文章信息/Info

Title:
Study on salt resistance and drought resistance of P5CS transgenic potato cultivar “Dongnong 303”
作者:
李葵花1;  高玉亮2;  吴京姬2
1.延边大学农学院,吉林延吉 133002; 2.延边朝鲜族自治州农业科学院,吉林龙井 133400
Author(s):
Li Kuihua; et al(131)
关键词:
马铃薯; P5CS基因; 耐盐性; 抗旱性; SOD; 丙二醛
Keywords:
-
分类号:
S532.03
DOI:
-
文献标志码:
A
摘要:
利用拟南芥Δ1-吡咯啉-5-羧酸合成酶基因(P5CS)进行了马铃薯品种“东农303”的遗传转化,在盐和干旱胁迫下研究转基因植株的耐盐性和抗旱性。经PCR扩增,证实P5CS基因已整合到马铃薯基因组中。用 150 mmol/L NaCl溶液和干旱(基质含水量为20%~25%)胁迫处理20 d,发现转基因植株叶片的脯氨酸含量显著增加,SOD活性明显上升,而丙二醛积累量则缓慢,从而说明该品种更适合生长在干旱和盐胁迫条件下。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2014-01-17
基金项目:国家自然科学基金(编号:31260470);延边大学科技发展计划项目启动金(编号:2011800-601010018)。
作者简介:李葵花(1970—),女,吉林延吉人,博士,副教授,从事园艺学研究。E-mail:khli@ybu.edu.cn。
更新日期/Last Update: 2014-11-25