|本期目录/Table of Contents|

[1]李葵花,高玉亮,吴京姬.转P5CS基因马铃薯“东农303”耐盐、抗旱性研究[J].江苏农业科学,2014,42(11):131-133.
 Li Kuihua,et al().Study on salt resistance and drought resistance of P5CS transgenic potato cultivar “Dongnong 303”[J].Jiangsu Agricultural Sciences,2014,42(11):131-133.
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P5CS基因马铃薯“东农303”耐盐、抗旱性研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年11期
页码:
131-133
栏目:
育种栽培与生理生化
出版日期:
2014-11-25

文章信息/Info

Title:
Study on salt resistance and drought resistance of P5CS transgenic potato cultivar “Dongnong 303”
作者:
李葵花1 高玉亮2 吴京姬2
1.延边大学农学院,吉林延吉 133002; 2.延边朝鲜族自治州农业科学院,吉林龙井 133400
Author(s):
Li Kuihuaet al(131)
关键词:
马铃薯P5CS基因耐盐性抗旱性SOD丙二醛
Keywords:
-
分类号:
S532.03
DOI:
-
文献标志码:
A
摘要:
利用拟南芥Δ1-吡咯啉-5-羧酸合成酶基因(P5CS)进行了马铃薯品种“东农303”的遗传转化,在盐和干旱胁迫下研究转基因植株的耐盐性和抗旱性。经PCR扩增,证实P5CS基因已整合到马铃薯基因组中。用 150 mmol/L NaCl溶液和干旱(基质含水量为20%~25%)胁迫处理20 d,发现转基因植株叶片的脯氨酸含量显著增加,SOD活性明显上升,而丙二醛积累量则缓慢,从而说明该品种更适合生长在干旱和盐胁迫条件下。
Abstract:
-

参考文献/References:

[1]张建锋. 盐碱地的生态修复研究[J]. 水土保持研究,2008,15(4):74-78.
[2]赵雅静,翁伯琦,王义祥,等. 植物对干旱胁迫的生理生态响应及其研究进展[J]. 福建稻麦科技,2009,27(2):45-50.
[3]Hamilton E W,Heckathorn S A. Mitochondrial adaptations to NaCl. Complex Ⅰ is protected by anti-oxidants and small heat shock proteins,whereas complex Ⅱ is protected by proline and betaine[J]. Plant Physiology,2001,126(3):1266-1274.
[4]Hong Z,Lakkineni K,Zhang Z,et al. Removal of feedback inhibition of Δ1-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress[J]. Plant Physiology,2000,122(4):1129-1136.
[5]Yoshiba Y,Kiyosue T,Katagiri T,et al. Correlation between the induction of a gene for Δ1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress[J]. The Plant Journal:for Cell and Molecular Biology,1995,7(5):751-760.
[6]Kavi Kishor P B,Hong Z L,Miao G H,et al. Overexpression of Δ1-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants[J]. Plant Physiology,1995,108:1387-1394
[7]Zhu B C,Su J,Chang M C,et al. Overexpression of a Δ1-pyrroline-5-carboxylate synthetase gene and analysis of tolerance to water-and salt-stress in transgenic rice[J]. Plant Science,1998,139:41-48.
[8]De Ronde J A,Cress W A,Krüger G H,et al. Photosynthetic response of transgenic soybean plants,containing an Arabidopsis P5CR gene,during heat and drought stress[J]. Journal of Plant Physiology,2004,161(11):1211-1224.
[9]Molinari H B,Marur C J,Filho J C,et al. Osmotic adjustment in transgenic citrus rootstock Carrizo citrange (Citrus sinensis Osb.×Poncirus trifoliata L. Raf.) overproducing proline[J]. Plant Science,2004,167(6):1375-1381.
[10]刘金龙,杨秀清,姚延梼. 铜锌元素与华北落叶松丙二醛含量关系的研究[J]. 山西农业大学学报:自然科学版,2003,23(3):212-215.
[11]Sherraf I,Tizroutine S,Chaput M H,et al. Production and characterization of intergeneric somatic hybrids through protoplast electrofusion between potato (Solanum tuberosum) and Lycopersicon pennellii[J]. Plant Cell Tissue and Organ Culture,1994,37(2):137-144.
[12]支立峰,陈明清,余涛,等. p5cs转化水稻细胞系的研究[J]. 湖北师范学院学报:自然科学版,2005,25(4):39-43,51.
[13]郭旭,雷江丽,唐益雄,等. 农作物抗渗透胁迫与P5CS基因工程研究进展[J]. 河南农业科学,2007(2):15-19.
[14]Hmida-Sayari A,Gargouri-Bouzid R,Bidani A,et al. Overexpression of Δ1-pyrroline-5-carboxylate synthetase increases proline production and confers salt tolerance in transgenic potato plants[J]. Plant Science,2005,169(4):746-752.

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备注/Memo

备注/Memo:
收稿日期:2014-01-17
基金项目:国家自然科学基金(编号:31260470);延边大学科技发展计划项目启动金(编号:2011800-601010018)。
作者简介:李葵花(1970—),女,吉林延吉人,博士,副教授,从事园艺学研究。E-mail:khli@ybu.edu.cn。
更新日期/Last Update: 2014-11-25